Molecules 2012, 17(10), 11748-11762; doi:10.3390/molecules171011748
Article

Optimization of Ultrasound-Assisted Extraction of Crude Oil from Winter Melon (Benincasa hispida) Seed Using Response Surface Methodology and Evaluation of Its Antioxidant Activity, Total Phenolic Content and Fatty Acid Composition

1email, 1,2,3,* email, 2email, 1email, 4email and 1,5email
Received: 27 August 2012; in revised form: 4 September 2012 / Accepted: 11 September 2012 / Published: 8 October 2012
(This article belongs to the Section Natural Products)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In the present study, ultrasound-assisted extraction of crude oil from winter melon seeds was investigated through response surface methodology (RSM). Process variables were power level (25–75%), temperature (45–55 °C) and sonication time (20–40 min). It was found that all process variables have significant (p < 0.05) effects on the response variable. A central composite design (CCD) was used to determine the optimum process conditions. Optimal conditions were identified as 65% power level, 52 °C temperature and 36 min sonication time for maximum crude yield (108.62 mg-extract/g-dried matter). The antioxidant activity, total phenolic content and fatty acid composition of extract obtained under optimized conditions were determined and compared with those of oil obtained by the Soxhlet method. It was found that crude extract yield (CEY) of ultrasound-assisted extraction was lower than that of the Soxhlet method, whereas antioxidant activity and total phenolic content of the extract obtained by ultrasound-assisted extraction were clearly higher than those of the Soxhlet extract. Furthermore, both extracts were rich in unsaturated fatty acids. The major fatty acids of the both extracts were linoleic acid and oleic acid.
Keywords: ultrasound-assisted extraction; winter melon; antioxidant activity; total phenolic content; fatty acid composition
PDF Full-text Download PDF Full-Text [2045 KB, uploaded 18 June 2014 19:55 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Bimakr, M.; Rahman, R.A.; Taip, F.S.; Adzahan, N.M.; Sarker, M.Z.I.; Ganjloo, A. Optimization of Ultrasound-Assisted Extraction of Crude Oil from Winter Melon (Benincasa hispida) Seed Using Response Surface Methodology and Evaluation of Its Antioxidant Activity, Total Phenolic Content and Fatty Acid Composition. Molecules 2012, 17, 11748-11762.

AMA Style

Bimakr M, Rahman RA, Taip FS, Adzahan NM, Sarker MZI, Ganjloo A. Optimization of Ultrasound-Assisted Extraction of Crude Oil from Winter Melon (Benincasa hispida) Seed Using Response Surface Methodology and Evaluation of Its Antioxidant Activity, Total Phenolic Content and Fatty Acid Composition. Molecules. 2012; 17(10):11748-11762.

Chicago/Turabian Style

Bimakr, Mandana; Rahman, Russly Abdul; Taip, Farah Saleena; Adzahan, Noranizan Mohd; Sarker, Md. Zaidul Islam; Ganjloo, Ali. 2012. "Optimization of Ultrasound-Assisted Extraction of Crude Oil from Winter Melon (Benincasa hispida) Seed Using Response Surface Methodology and Evaluation of Its Antioxidant Activity, Total Phenolic Content and Fatty Acid Composition." Molecules 17, no. 10: 11748-11762.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert