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Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin
Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynska dolina B-1, Bratislava 842 15, Slovakia
Institute for Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, Sofia 1113, Bulgaria
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojarov 10, Bratislava 832 32, Slovakia
School of Biological and Medical Sciences, University of St. Andrews, St. Andrews KY16 9TS, Scotland, UK
* Author to whom correspondence should be addressed.
Received: 31 October 2011; in revised form: 28 November 2011 / Accepted: 12 December 2011 / Published: 22 December 2011
Abstract: Lilium candidum L. extract (LE) is well known in folk medicine for the treatment of burns, ulcers, inflammations and for healing wounds. This work aims to clarify whether the genotoxic potential of the radiomimetic antibiotic zeocin (Zeo) could be modulated by LE. Our results indicate that LE exerts no cytotoxic, DNA-damaging and clastogenic activity in in Chlamydomonas reinhardtii, Pisum sativum L. and Hordeum vulgare L. test systems over a broad concentration range. Weak but statistically significant clastogenic effects due to the induction of micronuclei and chromosome aberrations have been observed in H. vulgare L. after treatment with 200 and 300 μg/mL LE. To discriminate protective from adverse action of LE different experimental designs have been used. Our results demonstrate that the treatment with mixtures of LE and Zeo causes an increase in the level of DNA damage, micronuclei and “metaphases with chromatid aberrations” (MwA). Clear evidence has been also obtained indicating that pretreatment with LE given 4 h before the treatment with Zeo accelerates the rejoining kinetics of Zeo-induced DNA damage in P. sativum L. and C. reinhardtii, and can decrease clastogenic effect of Zeo measured as frequencies of micronuclei and MwA in H. vulgare L. Here, we show for the first time that LE can modulate the genotoxic effects of zeocin. The molecular mode of action strongly depends on the experimental design and varies from synergistic to protective effect (adaptive response–AR). Our results also revealed that LE-induced AR to zeocin involves up-regulation of DSB rejoining in C. reinhardtii and P. sativum L. cells.
Keywords: zeocin; Lilium candidum L. extract; cytotoxicity; genotoxicity; adaptive response; DSBs rejoining
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Kopaskova, M.; Hadjo, L.; Yankulova, B.; Jovtchev, G.; Galova, E.; Sevcovicova, A.; Mucaji, P.; Miadokova, E.; Bryant, P.; Chankova, S. Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin. Molecules 2012, 17, 80-97.
Kopaskova M, Hadjo L, Yankulova B, Jovtchev G, Galova E, Sevcovicova A, Mucaji P, Miadokova E, Bryant P, Chankova S. Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin. Molecules. 2012; 17(1):80-97.
Kopaskova, Marcela; Hadjo, Lina; Yankulova, Bisera; Jovtchev, Gabriele; Galova, Eliska; Sevcovicova, Andrea; Mucaji, Pavel; Miadokova, Eva; Bryant, Peter; Chankova, Stephka. 2012. "Extract of Lillium candidum L. Can Modulate the Genotoxicity of the Antibiotic Zeocin." Molecules 17, no. 1: 80-97.