Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Molecules 2012, 17(1), 163-178; doi:10.3390/molecules17010163
Article

Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides

1, 1, 2, 2 and 1,3,*
Received: 29 November 2011; in revised form: 21 December 2011 / Accepted: 22 December 2011 / Published: 27 December 2011
(This article belongs to the Special Issue DNA-Templated Synthesis)
Download PDF [887 KB, uploaded 18 June 2014]
Abstract: We demonstrated that 5-vinyldeoxyuridine (VU) and 5-carboxyvinyldeoxyuridine (CVU) can be used to photoligate a longer oligonucleotide (ODN) from smaller ODNs on a template. By performing irradiation at 366 nm, these artificial nucleotides make photoligated ODNs with high efficiency without any side reactions. Moreover, by performing irradiation at 312 nm, these photoligated ODNs were reversed to the original ODN. VU needs to be irradiated 366 nm for 6 h, but CVU needs to be irradiated at 366 nm for 15 min. Finally, we made a self-assembled structure with an ODN containing CVU and observed the photoligated ODN by photoirradiation.
Keywords: photochemical ligation; 5-carboxyvinyldeoxyuridine; 5-vinyldeoxyuridine; DNA nanotechnology photochemical ligation; 5-carboxyvinyldeoxyuridine; 5-vinyldeoxyuridine; DNA nanotechnology
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Nakamura, S.; Ogasawara, S.; Matuda, S.; Saito, I.; Fujimoto, K. Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides. Molecules 2012, 17, 163-178.

AMA Style

Nakamura S, Ogasawara S, Matuda S, Saito I, Fujimoto K. Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides. Molecules. 2012; 17(1):163-178.

Chicago/Turabian Style

Nakamura, Shigetaka; Ogasawara, Shinzi; Matuda, Shigeo; Saito, Isao; Fujimoto, Kenzo. 2012. "Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides." Molecules 17, no. 1: 163-178.


Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert