Open AccessThis article is
- freely available
Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides
School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan
Department of Synthetic Chemistry and Biological Chemistry, Factly of Enginnering, Kyoto University, Kyoto 606-8501, Japan
Research Center for Bio-Architecture, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan
* Author to whom correspondence should be addressed.
Received: 29 November 2011; in revised form: 21 December 2011 / Accepted: 22 December 2011 / Published: 27 December 2011
Abstract: We demonstrated that 5-vinyldeoxyuridine (VU) and 5-carboxyvinyldeoxyuridine (CVU) can be used to photoligate a longer oligonucleotide (ODN) from smaller ODNs on a template. By performing irradiation at 366 nm, these artificial nucleotides make photoligated ODNs with high efficiency without any side reactions. Moreover, by performing irradiation at 312 nm, these photoligated ODNs were reversed to the original ODN. VU needs to be irradiated 366 nm for 6 h, but CVU needs to be irradiated at 366 nm for 15 min. Finally, we made a self-assembled structure with an ODN containing CVU and observed the photoligated ODN by photoirradiation.
Keywords: photochemical ligation; 5-carboxyvinyldeoxyuridine; 5-vinyldeoxyuridine; DNA nanotechnology
Article StatisticsClick here to load and display the download statistics.
Notes: Multiple requests from the same IP address are counted as one view.
Cite This Article
MDPI and ACS Style
Nakamura, S.; Ogasawara, S.; Matuda, S.; Saito, I.; Fujimoto, K. Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides. Molecules 2012, 17, 163-178.
Nakamura S, Ogasawara S, Matuda S, Saito I, Fujimoto K. Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides. Molecules. 2012; 17(1):163-178.
Nakamura, Shigetaka; Ogasawara, Shinzi; Matuda, Shigeo; Saito, Isao; Fujimoto, Kenzo. 2012. "Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides." Molecules 17, no. 1: 163-178.