Molecules 2012, 17(1), 163-178; doi:10.3390/molecules17010163
Article

Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides

Shigetaka Nakamura 1 , Shinzi Ogasawara 1 , Shigeo Matuda 2 , Isao Saito 2  and Kenzo Fujimoto 1,3,* email
1 School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan 2 Department of Synthetic Chemistry and Biological Chemistry, Factly of Enginnering, Kyoto University, Kyoto 606-8501, Japan 3 Research Center for Bio-Architecture, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan
* Author to whom correspondence should be addressed.
Received: 29 November 2011; in revised form: 21 December 2011 / Accepted: 22 December 2011 / Published: 27 December 2011
(This article belongs to the Special Issue DNA-Templated Synthesis)
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Abstract: We demonstrated that 5-vinyldeoxyuridine (VU) and 5-carboxyvinyldeoxyuridine (CVU) can be used to photoligate a longer oligonucleotide (ODN) from smaller ODNs on a template. By performing irradiation at 366 nm, these artificial nucleotides make photoligated ODNs with high efficiency without any side reactions. Moreover, by performing irradiation at 312 nm, these photoligated ODNs were reversed to the original ODN. VU needs to be irradiated 366 nm for 6 h, but CVU needs to be irradiated at 366 nm for 15 min. Finally, we made a self-assembled structure with an ODN containing CVU and observed the photoligated ODN by photoirradiation.
Keywords: photochemical ligation; 5-carboxyvinyldeoxyuridine; 5-vinyldeoxyuridine; DNA nanotechnology

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Cite This Article

MDPI and ACS Style

Nakamura, S.; Ogasawara, S.; Matuda, S.; Saito, I.; Fujimoto, K. Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides. Molecules 2012, 17, 163-178.

AMA Style

Nakamura S, Ogasawara S, Matuda S, Saito I, Fujimoto K. Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides. Molecules. 2012; 17(1):163-178.

Chicago/Turabian Style

Nakamura, Shigetaka; Ogasawara, Shinzi; Matuda, Shigeo; Saito, Isao; Fujimoto, Kenzo. 2012. "Template Directed Reversible Photochemical Ligation of Oligodeoxynucleotides." Molecules 17, no. 1: 163-178.

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