Molecules 2011, 16(9), 7583-7592; doi:10.3390/molecules16097583
Article

Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract

1, 1, 2,3,* email, 4email, 2, 2 and 2email
Received: 2 July 2011; Accepted: 15 August 2011 / Published: 5 September 2011
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Background and objective: Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation. Results: The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone. Conclusions: The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.
Keywords: bioactive compounds; bioassay-directed isolation; natural products extraction; fractionation
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MDPI and ACS Style

Jothy, S.L.; Zakaria, Z.; Chen, Y.; Lau, Y.L.; Latha, L.Y.; Shin, L.N.; Sasidharan, S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules 2011, 16, 7583-7592.

AMA Style

Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Shin LN, Sasidharan S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules. 2011; 16(9):7583-7592.

Chicago/Turabian Style

Jothy, Subramanion L.; Zakaria, Zuraini; Chen, Yeng; Lau, Yee Ling; Latha, Lachimanan Yoga; Shin, Lai Ngit; Sasidharan, Sreenivasan. 2011. "Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract." Molecules 16, no. 9: 7583-7592.

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