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Molecules 2011, 16(9), 7583-7592; doi:10.3390/molecules16097583
Article

Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract

1, 1, 2,3,* , 4
,
2, 2 and 2
1 Biological Program, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia 2 Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia 3 Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia 4 Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
* Author to whom correspondence should be addressed.
Received: 2 July 2011 / Accepted: 15 August 2011 / Published: 5 September 2011
(This article belongs to the Section Natural Products)
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Abstract

Background and objective: Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation. Results: The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone. Conclusions: The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.
Keywords: bioactive compounds; bioassay-directed isolation; natural products extraction; fractionation bioactive compounds; bioassay-directed isolation; natural products extraction; fractionation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Jothy, S.L.; Zakaria, Z.; Chen, Y.; Lau, Y.L.; Latha, L.Y.; Shin, L.N.; Sasidharan, S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules 2011, 16, 7583-7592.

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