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Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract
Biological Program, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia
Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia
Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
* Author to whom correspondence should be addressed.
Received: 2 July 2011; Accepted: 15 August 2011 / Published: 5 September 2011
Abstract: Background and objective: Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation. Results: The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone. Conclusions: The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.
Keywords: bioactive compounds; bioassay-directed isolation; natural products extraction; fractionation
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MDPI and ACS Style
Jothy, S.L.; Zakaria, Z.; Chen, Y.; Lau, Y.L.; Latha, L.Y.; Shin, L.N.; Sasidharan, S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules 2011, 16, 7583-7592.
Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Shin LN, Sasidharan S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules. 2011; 16(9):7583-7592.
Jothy, Subramanion L.; Zakaria, Zuraini; Chen, Yeng; Lau, Yee Ling; Latha, Lachimanan Yoga; Shin, Lai Ngit; Sasidharan, Sreenivasan. 2011. "Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract." Molecules 16, no. 9: 7583-7592.