Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract
Abstract
:1. Introduction
2. Results and Discussion
Test | Seed extract | Observation |
---|---|---|
Reducing Sugar | – | dark greenish |
Anthraquinone | + | deep red coloration of aqueous layer |
Terpenoids | + | deep red coloration |
Flavonoids | + | colorless |
Saponins | + | persistence of frothing |
Tannins | + | dark greenish grey coloration |
Alkaloids | – | dark greenish |
Microorganism | Zone of inhibition (mm) | ||
---|---|---|---|
Crude extract | Active fractions | Miconazole nitrate | |
Candida albicans | 21 | 250 µg/mL–18 | 22 |
3. Experimental
3.1. Plant Collection
3.2. Solvent Extraction
3.3. Preliminary Phytochemicals Screening
3.4. Microorganism
3.5. Column Chromatography
3.6. Antimicrobial Activity
3.6.1. Disk Diffusion Technique
3.7. Isolation of Antiyeast Substances from Crude Extract of C. fistula
3.7.1. High-Performance Liquid Chromatography (HPLC) Separation
3.7.2. Identification of Compound
3.7.3. Effect of the Active Fraction on C. albicans Cells by Scanning Electron Microscopy (SEM) Study
4. Conclusion
Acknowledgements
References
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Jothy, S.L.; Zakaria, Z.; Chen, Y.; Lau, Y.L.; Latha, L.Y.; Shin, L.N.; Sasidharan, S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules 2011, 16, 7583-7592. https://doi.org/10.3390/molecules16097583
Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Shin LN, Sasidharan S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules. 2011; 16(9):7583-7592. https://doi.org/10.3390/molecules16097583
Chicago/Turabian StyleJothy, Subramanion L., Zuraini Zakaria, Yeng Chen, Yee Ling Lau, Lachimanan Yoga Latha, Lai Ngit Shin, and Sreenivasan Sasidharan. 2011. "Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract" Molecules 16, no. 9: 7583-7592. https://doi.org/10.3390/molecules16097583