Abstract: Background and objective: Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation. Results: The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone. Conclusions: The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.
Keywords: bioactive compounds; bioassay-directed isolation; natural products extraction; fractionation
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Jothy, S.L.; Zakaria, Z.; Chen, Y.; Lau, Y.L.; Latha, L.Y.; Shin, L.N.; Sasidharan, S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules 2011, 16, 7583-7592.
Jothy SL, Zakaria Z, Chen Y, Lau YL, Latha LY, Shin LN, Sasidharan S. Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract. Molecules. 2011; 16(9):7583-7592.
Jothy, Subramanion L.; Zakaria, Zuraini; Chen, Yeng; Lau, Yee Ling; Latha, Lachimanan Yoga; Shin, Lai Ngit; Sasidharan, Sreenivasan. 2011. "Bioassay-Directed Isolation of Active Compounds with Antiyeast Activity from a Cassia fistula Seed Extract." Molecules 16, no. 9: 7583-7592.