Molecules 2011, 16(8), 6656-6666; doi:10.3390/molecules16086656
Article

In Vitro Synergy of Biochanin A and Ciprofloxacin against Clinical Isolates of Staphylococcus aureus

1,†, 1,†, 1,†, 1,†, 1,†, 2,†, 1,†, 1, 1, 1, 1, 1,* email and 1,3,4,* email
Received: 11 July 2011; in revised form: 30 July 2011 / Accepted: 2 August 2011 / Published: 5 August 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Many clinical isolates of Staphylococcus aureus (S. aureus) are resistant to numerous antimicrobials, including the fluoroquinolones (FQs). Flavonoids such as biochanin A (BCA) are compounds that are naturally present in fruits, vegetables, and plant-derived beverages. The goal of this investigation was to study the possible synergy between the antimicrobial agents BCA and ciprofloxacin (CPFX) when used in combination; CPFX was chosen as a representative FQ compound. We used S. aureus strain ATCC 25923 and 11 fluoroquinolone (FQ)-resistant methicillin-resistant S. aureus (MRSA) strains. Results from the drug susceptibility testing and checkerboard assays show that the minimum inhibitory concentration (MIC) of BCA ranged from 64 µg/mL to 512 µg/mL. When BCA was combined with CPFX, the fractional inhibitory concentration index (FICI) data showed that there was synergy in all 12 of the S. aureus strains tested. No antagonistic activity was observed in any of the strains tested. The results of time-kill tests and agar diffusion tests confirm that there was synergy between BCA and CPFX against S. aureus strains. These results suggest that BCA can be combined with FQs to produce a powerful antimicrobial agent.
Keywords: antimycobacterial; biochanin A; ciprofloxacin; Staphylococcus aureus; synergy
PDF Full-text Download PDF Full-Text [364 KB, uploaded 18 June 2014 19:39 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Liu, G.; Liang, J.-C.; Wang, X.-L.; Li, Z.-H.; Wang, W.; Guo, N.; Wu, X.-P.; Shen, F.-G.; Xing, M.-X.; Liu, L.-H.; Li, L.; Liu, M.-Y.; Yu, L. In Vitro Synergy of Biochanin A and Ciprofloxacin against Clinical Isolates of Staphylococcus aureus. Molecules 2011, 16, 6656-6666.

AMA Style

Liu G, Liang J-C, Wang X-L, Li Z-H, Wang W, Guo N, Wu X-P, Shen F-G, Xing M-X, Liu L-H, Li L, Liu M-Y, Yu L. In Vitro Synergy of Biochanin A and Ciprofloxacin against Clinical Isolates of Staphylococcus aureus. Molecules. 2011; 16(8):6656-6666.

Chicago/Turabian Style

Liu, Guoxing; Liang, Jun-Chao; Wang, Xue-Lin; Li, Zhao-Hui; Wang, Wei; Guo, Na; Wu, Xiu-Ping; Shen, Feng-Ge; Xing, Ming-Xun; Liu, Li-Hui; Li, Lei; Liu, Ming-Yuan; Yu, Lu. 2011. "In Vitro Synergy of Biochanin A and Ciprofloxacin against Clinical Isolates of Staphylococcus aureus." Molecules 16, no. 8: 6656-6666.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert