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• Neves, M
• Silva, M
• Souza-Motta, C
• Spier, M
• Soccol, C
• Porto, T
• Moreira, K
• Porto, A
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• Neves, M
• Silva, M
• Souza-Motta, C
• Spier, M
• Soccol, C
• Porto, T
• Moreira, K
• Porto, A
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• Neves, M
• Silva, M
• Souza-Motta, C
• Spier, M
• Soccol, C
• Porto, T
• Moreira, K
• Porto, A

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Molecules 2011, 16(6), 4807-4817; doi:10.3390/molecules16064807

Article

Lichtheimia blakesleeana as a New Potencial Producer of Phytase and Xylanase

Maria Luiza Carvalho Neves ^1,2 , Milena Fernandes da Silva ³ , Cristina Maria Souza-Motta ⁴ , Michele Rigon Spier ⁵ , Carlos Ricardo Soccol ⁵ , Tatiana Souza Porto ⁶ , Keila Aparecida Moreira ⁶  and Ana Lúcia Figueiredo Porto ^1,3,*

¹ Renorbio/ UECE, Av. Paranjana, 1.700, 60740-000, Fortaleza, Ceará, Brazil ² Department of Pathology, University of Pernambuco, ICB-UPE, Rua Arnóbio Marques, 310, 50100-130, Recife, Pernambuco, Brazil ³ Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n., 52171-900, Recife, Pernambuco, Brazil ⁴ Department of Mycology, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, Pernambuco, Brazil ⁵ Department of Biotechnology and Bioprocess, Federal University of Paraná, P.O. Box 19011, 81531-970, Curitiba, Paraná, Brazil ⁶ Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Avenida Bom Pastor, s/n, Boa Vista, 55292-270, Garanhuns, Pernambuco, Brazil

* Author to whom correspondence should be addressed.

Received: 19 May 2011; in revised form: 7 June 2011 / Accepted: 8 June 2011 / Published: 9 June 2011

Download PDF Full-Text [295 KB, uploaded 9 June 2011 17:10 CEST]

Abstract: Brazil is known for its great potential for production of renewable resources such as agro-industrial residues. These residues can be used as alternative sources of new products. Meanwhile, solid-state fermentation, with its advantages of energy conservation and pollution reduction, has been identified as a process of great potential for the production of bioactive compounds, especially enzymes. In the present work, a 2³ factorial design was used to evaluate the effects of pH, temperature and moisture on the production of phytase and xylanase by Lichtheimia blakesleeana URM 5604 through the fermentation of citrus pulp. Statistical analyses of the results showed that the only the pH influenced the production of these enzymes, with the best phytase production (264.68 U/g) ocurring at pH 6.0, 34 °C, initial moisture 50%, after 48 hours of culture. The best conditions for xylanase production (397.82 U/g) were fermentation for 120 hours at pH 4.0, 26 °C and initial moisture of 70%. The best parameters for the simultaneous production of phytase (226.92 U/g) and xylanase (215.59 U/g) were determined to be initial moisture of 50%, pH 6.0, 26 °C, and 48 hours of fermentation.

Keywords: phytase; xylanase; citrus pulp; solid-state fermentation; Lichtheimia blakesleeana

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