Next Article in Journal
Effects of Fractionation and Combinatorial Evaluation of Tamarindus indica Fractions for Antibacterial Activity
Previous Article in Journal
Synthesis and In Vitro Antiproliferative Activity of Novel Androst-5-ene Triazolyl and Tetrazolyl Derivatives
Molecules 2011, 16(6), 4807-4817; doi:10.3390/molecules16064807
Article

Lichtheimia blakesleeana as a New Potencial Producer of Phytase and Xylanase

1,2, 3, 4, 5, 5, 6, 6 and 1,3,*
Received: 19 May 2011 / Revised: 7 June 2011 / Accepted: 8 June 2011 / Published: 9 June 2011
Download PDF [295 KB, uploaded 18 June 2014]

Abstract

Brazil is known for its great potential for production of renewable resources such as agro-industrial residues. These residues can be used as alternative sources of new products. Meanwhile, solid-state fermentation, with its advantages of energy conservation and pollution reduction, has been identified as a process of great potential for the production of bioactive compounds, especially enzymes. In the present work, a 23 factorial design was used to evaluate the effects of pH, temperature and moisture on the production of phytase and xylanase by Lichtheimia blakesleeana URM 5604 through the fermentation of citrus pulp. Statistical analyses of the results showed that the only the pH influenced the production of these enzymes, with the best phytase production (264.68 U/g) ocurring at pH 6.0, 34 °C, initial moisture 50%, after 48 hours of culture. The best conditions for xylanase production (397.82 U/g) were fermentation for 120 hours at pH 4.0, 26 °C and initial moisture of 70%. The best parameters for the simultaneous production of phytase (226.92 U/g) and xylanase (215.59 U/g) were determined to be initial moisture of 50%, pH 6.0, 26 °C, and 48 hours of fermentation.
Keywords: phytase; xylanase; citrus pulp; solid-state fermentation; Lichtheimia blakesleeana phytase; xylanase; citrus pulp; solid-state fermentation; Lichtheimia blakesleeana
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Neves, M.L.C.; Silva, M.F.; Souza-Motta, C.M.; Spier, M.R.; Soccol, C.R.; Porto, T.S.; Moreira, K.A.; Porto, A.L.F. Lichtheimia blakesleeana as a New Potencial Producer of Phytase and Xylanase. Molecules 2011, 16, 4807-4817.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert