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Molecules 2011, 16(6), 4539-4548; doi:10.3390/molecules16064539
Article

Phytochemical and Cytotoxic Investigations of Curcuma mangga Rhizomes

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Received: 29 March 2011 / Revised: 18 April 2011 / Accepted: 20 April 2011 / Published: 31 May 2011
(This article belongs to the Section Metabolites)
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Abstract

Investigations on the cytotoxic effects of the crude methanol and fractionated extracts (hexane, ethyl acetate) C. mangga against six human cancer cell lines, namely the hormone-dependent breast cell line (MCF-7), nasopharyngeal epidermoid cell line (KB), lung cell line (A549), cervical cell line (Ca Ski), colon cell lines (HCT 116 and HT-29), and one non-cancer human fibroblast cell line (MRC-5) were conducted using an in-vitro neutral red cytotoxicity assay. The crude methanol and fractionated extracts (hexane and ethyl acetate) displayed good cytotoxic effects against MCF-7, KB, A549, Ca Ski and HT-29 cell lines, but exerted no damage on the MRC-5 line. Chemical investigation from the hexane and ethyl acetate fractions resulted in the isolation of seven pure compounds, namely (E)-labda-8(17),12-dien-15,16-dial (1), (E)-15,16-bisnor-labda-8(17),11-dien-13-on (2), zerumin A (3), β-sitosterol, curcumin, demethoxycurcumin and bis-demethoxycurcumin. Compounds 1 and 3 exhibited high cytotoxic effects against all six selected cancer cell lines, while compounds 2 showed no anti-proliferative activity on the tested cell lines. Compound 1 also demonstrated strong cytotoxicity against the normal cell line MRC-5. This paper reports for the first time the cytotoxic activities of C. mangga extracts on KB, A549, Ca Ski, HT-29 and MRC-5, and the occurrence of compound 2 and 3 in C. mangga.
Keywords: Curcuma mangga; Zingiberaceae; labdane diterpene; neutral red cytotoxicity assay; cancer cell lines Curcuma mangga; Zingiberaceae; labdane diterpene; neutral red cytotoxicity assay; cancer cell lines
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Malek, S.N.A.; Lee, G.S.; Hong, S.L.; Yaacob, H.; Wahab, N.A.; Faizal Weber, J.-F.; Shah, S.A.A. Phytochemical and Cytotoxic Investigations of Curcuma mangga Rhizomes. Molecules 2011, 16, 4539-4548.

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