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Phytochemical and Cytotoxic Investigations of Curcuma mangga Rhizomes
Institute of Biological Sciences, University of Malaysia, 50603 Kuala Lumpur, Malaysia
International University College of Nursing, L1.10, Cova Square, Jalan Teknologi, Kota Damansara, PJU5, 47810 Petaling Jaya, Selangor Darul Ehsan, Malaysia
Centre for Foundation Studies in Science, University of Malaysia, 50603 Kuala Lumpur, Malaysia
Research Institute of Natural Products for Drug Discovery (RiND), Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia
* Author to whom correspondence should be addressed.
Received: 29 March 2011; in revised form: 18 April 2011 / Accepted: 20 April 2011 / Published: 31 May 2011
Abstract: Investigations on the cytotoxic effects of the crude methanol and fractionated extracts (hexane, ethyl acetate) C. mangga against six human cancer cell lines, namely the hormone-dependent breast cell line (MCF-7), nasopharyngeal epidermoid cell line (KB), lung cell line (A549), cervical cell line (Ca Ski), colon cell lines (HCT 116 and HT-29), and one non-cancer human fibroblast cell line (MRC-5) were conducted using an in-vitro neutral red cytotoxicity assay. The crude methanol and fractionated extracts (hexane and ethyl acetate) displayed good cytotoxic effects against MCF-7, KB, A549, Ca Ski and HT-29 cell lines, but exerted no damage on the MRC-5 line. Chemical investigation from the hexane and ethyl acetate fractions resulted in the isolation of seven pure compounds, namely (E)-labda-8(17),12-dien-15,16-dial (1), (E)-15,16-bisnor-labda-8(17),11-dien-13-on (2), zerumin A (3), β-sitosterol, curcumin, demethoxycurcumin and bis-demethoxycurcumin. Compounds 1 and 3 exhibited high cytotoxic effects against all six selected cancer cell lines, while compounds 2 showed no anti-proliferative activity on the tested cell lines. Compound 1 also demonstrated strong cytotoxicity against the normal cell line MRC-5. This paper reports for the first time the cytotoxic activities of C. mangga extracts on KB, A549, Ca Ski, HT-29 and MRC-5, and the occurrence of compound 2 and 3 in C. mangga.
Keywords: Curcuma mangga; Zingiberaceae; labdane diterpene; neutral red cytotoxicity assay; cancer cell lines
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MDPI and ACS Style
Malek, S.N.A.; Lee, G.S.; Hong, S.L.; Yaacob, H.; Wahab, N.A.; Faizal Weber, J.-F.; Shah, S.A.A. Phytochemical and Cytotoxic Investigations of Curcuma mangga Rhizomes. Molecules 2011, 16, 4539-4548.
Malek SNA, Lee GS, Hong SL, Yaacob H, Wahab NA, Faizal Weber J-F, Shah SAA. Phytochemical and Cytotoxic Investigations of Curcuma mangga Rhizomes. Molecules. 2011; 16(6):4539-4548.
Malek, Sri Nurestri A.; Lee, Guan Serm; Hong, Sok Lai; Yaacob, Hashim; Wahab, Norhanom Abdul; Faizal Weber, Jean-Frédéric; Shah, Syed Adnan Ali. 2011. "Phytochemical and Cytotoxic Investigations of Curcuma mangga Rhizomes." Molecules 16, no. 6: 4539-4548.