Molecules 2011, 16(6), 4358-4370; doi:10.3390/molecules16064358
Article

Halomonas sp. OKOH—A Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay—Produces a Polysaccharide Bioflocculant: Partial Characterization and Biochemical Analysis of Its Properties

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Received: 23 March 2011; in revised form: 19 May 2011 / Accepted: 20 May 2011 / Published: 25 May 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A bioflocculant-producing bacterium isolated from seawater was identified based on 16S rRNA gene nucleotide sequence to have 99% similarity to that of Halomonas sp. Au160H and the nucleotide sequence was deposited as Halomonas sp. OKOH (Genbank accession number is HQ875722). Influences of carbon source, nitrogen source, salt ions and pH on flocculating activity were investigated. The bioflocculant was optimally produced when glucose (87% flocculating activity) and urea (88% flocculating activity) were used as sources of carbon and nitrogen, respectively. Also, initial pH of 7.0 and Ca2+ supported optimal production of the bioflocculant with flocculating activities of 87% respectively. Chemical analyses revealed the bioflocculant to be a polysaccharide.
Keywords: Halomonas sp. OKOH; bioflocculant; polysaccharide
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MDPI and ACS Style

Mabinya, L.V.; Cosa, S.; Mkwetshana, N.; Okoh, A.I. Halomonas sp. OKOH—A Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay—Produces a Polysaccharide Bioflocculant: Partial Characterization and Biochemical Analysis of Its Properties. Molecules 2011, 16, 4358-4370.

AMA Style

Mabinya LV, Cosa S, Mkwetshana N, Okoh AI. Halomonas sp. OKOH—A Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay—Produces a Polysaccharide Bioflocculant: Partial Characterization and Biochemical Analysis of Its Properties. Molecules. 2011; 16(6):4358-4370.

Chicago/Turabian Style

Mabinya, Leonard V.; Cosa, Sekelwa; Mkwetshana, Noxolo; Okoh, Anthony I. 2011. "Halomonas sp. OKOH—A Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay—Produces a Polysaccharide Bioflocculant: Partial Characterization and Biochemical Analysis of Its Properties." Molecules 16, no. 6: 4358-4370.

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