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Molecules 2011, 16(5), 4295-4304; doi:10.3390/molecules16054295
Article

Transformation of Geniposide into Genipin by Immobilized β-Glucosidase in a Two-Phase Aqueous-Organic System

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Received: 15 February 2011 / Revised: 3 May 2011 / Accepted: 13 May 2011 / Published: 24 May 2011
(This article belongs to the Section Metabolites)
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Abstract

Genipin is the bioactive compound of geniposide and a natural cross-linking agent. In order to improve the preparation process of genipin, the hydrolysis of geniposide to genipin by immobilized β-glucosidase in an aqueous-organic two-phase system was studied. β-Glucosidase was immobilized by the crosslinking-embedding method using sodium alginate as the carrier. The optimum reaction temperature, pH value and time were 55 °C, 4.5 and 2.5 h, respectively. To reduce genipin hydrolysis and byproduct production the reaction was carried out in an aqueous-organic two-phase system comprising ethyl acetate and sodium acetate buffer. The product was analyzed by HPLC, UV, IR, and NMR. The yield of genipin was 47.81% and its purity was over 98% (HPLC).
Keywords: immobilized β-glucosidase; geniposide; genipin; aqueous-organic two-phase system immobilized β-glucosidase; geniposide; genipin; aqueous-organic two-phase system
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yang, Y.-S.; Zhang, T.; Yu, S.-C.; Ding, Y.; Zhang, L.-Y.; Qiu, C.; Jin, D. Transformation of Geniposide into Genipin by Immobilized β-Glucosidase in a Two-Phase Aqueous-Organic System. Molecules 2011, 16, 4295-4304.

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