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Molecules 2011, 16(4), 3152-3167; doi:10.3390/molecules16043152

Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae)

* , ,  and
Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia
* Author to whom correspondence should be addressed.
Received: 14 March 2011 / Revised: 12 April 2011 / Accepted: 14 April 2011 / Published: 14 April 2011
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Extracts of Melittis melissophyllum leaves in ether, chloroform, ethyl acetate, n-butanol and water were evaporated to dryness and dissolved in 50% ethanol to make 10% (w/v) solutions. The potential protective action of the extracts was assessed by the corresponding in vitro and in vivo tests. In the in vitro experiments extracts were tested as potential scavengers of free radicals (DPPH, O2·-, NO, and OH radicals), as well as inhibitors of liposomal peroxidation (LPx). The results obtained show that all extracts (exept n-BuOH extract) are good scavengers of radicals and reduce LPx intensity in liposomes, which points to their protective (antioxidant) activity. In vivo experiments were concerned with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx) in liver homogenate and blood-hemolysate of experimental animals after their treatment with extracts of M. melissophyllum leaves, or in combination with CCl4. On the basis of the results obtained it can be concluded that the examined extracts have protective (antioxidative) effect and this antioxidative behaviour is more pronounced in liver than in blood-hemolysate. The reason is probably the fact that liver contains other enzymatic systems, which can also participate in the antioxidative mechanism. Of all the extracts the H2O one showed the highest protective activity.
Keywords: Melittis melissophyllum L. extracts; free radicals; oxidative stress; in vitro tests; in vivo experiments Melittis melissophyllum L. extracts; free radicals; oxidative stress; in vitro tests; in vivo experiments
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kaurinovic, B.; Popovic, M.; Vlaisavljevic, S.; Raseta, M. Antioxidant Activities of Melittis melissophyllum L. (Lamiaceae). Molecules 2011, 16, 3152-3167.

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