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Investigation of the Interactions between the Hydrophobic Cavities of Cyclodextrins and Pullulanase
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
Food Science Department, Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China
* Author to whom correspondence should be addressed.
Received: 2 March 2011; in revised form: 30 March 2011 / Accepted: 1 April 2011 / Published: 7 April 2011
Abstract: The effects of cyclodextrins and derivatives on the activity and structure of pullulanase were investigated in this study. Our results showed that cyclodextrins and derivatives decreased the activity of pullulanase. This decrease was attributed to the interaction between the hydrophobic cavities of cyclodextrins and pullulanase. The hydrophobic cavity was confirmed to encapsulate the groups of pullulanase molecules by the addition of competitive guests. The results obtained from fluorescence spectroscopy analysis showed that β-CD showed more efficient interactions with pullulanase molecules and the side chain groups of cyclodextrin significantly prevented the interaction between the hydrophobic cavities of β-CD and pullulanase molecules. These findings suggest that the geometric dimension of hydrophobic cavities was crucial for matching between cyclodextrins and pullulanase and steric hindrance caused by side chains led to the decrease of the interaction.
Keywords: hydrophobic cavity; cyclodextrin; pullulanase; complex; fluorescence spectra
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MDPI and ACS Style
Yu, B.; Wang, J.; Zhang, H.; Jin, Z. Investigation of the Interactions between the Hydrophobic Cavities of Cyclodextrins and Pullulanase. Molecules 2011, 16, 3010-3017.
Yu B, Wang J, Zhang H, Jin Z. Investigation of the Interactions between the Hydrophobic Cavities of Cyclodextrins and Pullulanase. Molecules. 2011; 16(4):3010-3017.
Yu, Bo; Wang, Jinpeng; Zhang, Huanxin; Jin, Zhengyu. 2011. "Investigation of the Interactions between the Hydrophobic Cavities of Cyclodextrins and Pullulanase." Molecules 16, no. 4: 3010-3017.