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Molecules 2011, 16(4), 2743-2753; doi:10.3390/molecules16042743

Molecular Characterization of Arabinoxylans from Hull-Less Barley Milling Fractions

Grain College, Henan University of Technology, Zhengzhou 450052, Henan Province, China
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Received: 27 December 2010 / Revised: 11 March 2011 / Accepted: 17 March 2011 / Published: 24 March 2011
Download PDF [242 KB, 18 June 2014; original version 18 June 2014]

Abstract

Arabinoxylans were prepared from different hull-less barley milling fractions (bran, shorts and flour). The yields of hull-less bran arabinoxylan (HBB-AX), shorts arabinoxylan (HBS-AX) and flour arabinoxylan (HBF-AX) were 8.42%, 4.08% and 2.13% respectively. Sugar composition analysis showed that arabinose and xylose were the main sugars. HBF-AX had the highest Ara/Xyl ratio, followed by HBS-AX and HBB-AX. Size exclusion chromatography analysis (HPSEC) showed that HBF-AX had the highest molecular weight, followed by HBS-AX and HBB-AX, which had the lowest molecular weight. Aqueous solutions of HBB-AX, HBS-AX and HBF-AX had higher molecular weight(Mw) than in 0.5 mol/L NaOH and 1.0 mol/L NaOH. Dynamic light scattering analysis (DLS) showed that HBB-AX, HBS-AX and HBF-AX had existed in two states in distilled water, 0.5 mol/LNaOH, 1.0 mol/L NaOH, and DMSO/H2O (90/10), an unaggregated state and an aggregated state, with the latter predominating.
Keywords: arabinoxylan; hull-less barley; size exclusion chromatography analysis; dynamic light scattering analysis arabinoxylan; hull-less barley; size exclusion chromatography analysis; dynamic light scattering analysis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zheng, X.; Li, L.; Wang, X. Molecular Characterization of Arabinoxylans from Hull-Less Barley Milling Fractions. Molecules 2011, 16, 2743-2753.

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