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Molecules 2011, 16(3), 2431-2442; doi:10.3390/molecules16032431
Article

Bioflocculant Production by Virgibacillus sp. Rob Isolated from the Bottom Sediment of Algoa Bay in the Eastern Cape, South Africa

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1 Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa 2 Division of Microbiology, University of KwaZulu Natal, Durban Westville, South Africa 3 Department of Chemistry, University of Fort Hare, Alice, South Africa 4 South African Environmental Observation Network, SAEON, Elwandle Node, 18 Somerset Street, Grahamstown, 6140, South Africa
* Author to whom correspondence should be addressed.
Received: 21 December 2011 / Revised: 10 March 2011 / Accepted: 11 March 2011 / Published: 14 March 2011
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Abstract

A bioflocculant-producing marine bacterium previously isolated from marine sediment of Algoa Bay was screened for flocculant production. Comparative analysis of 16S rDNA sequence identified the isolate to have 99% similarity to Virgibacillus sp. XQ-1 and it was deposited in the GenBank as Virgibacillus sp. Rob with accession number HQ537127. The bacterium produced biflocculants optimally in glucose (70.4%) and peptone (70.4%) as sole sources of carbon and nitrogen, alkaline pH (12) (74%); and the presence of Fe2+ (74%). Chemical analysis of the bioflocculant revealed it to be a polysaccharide.
Keywords: Virgibacillus sp.; bioflocculant; flocculating activity; polysaccharide Virgibacillus sp.; bioflocculant; flocculating activity; polysaccharide
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Cosa, S.; Mabinya, L.V.; Olaniran, A.O.; Okoh, O.O.; Bernard, K.; Deyzel, S.; Okoh, A.I. Bioflocculant Production by Virgibacillus sp. Rob Isolated from the Bottom Sediment of Algoa Bay in the Eastern Cape, South Africa. Molecules 2011, 16, 2431-2442.

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