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Molecules 2011, 16(12), 10078-10092; doi:10.3390/molecules161210078
Article

Genetic Diversity and Identification of Chinese-Grown Pecan Using ISSR and SSR Markers

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Received: 19 October 2011; in revised form: 25 November 2011 / Accepted: 28 November 2011 / Published: 6 December 2011
(This article belongs to the Section Organic Synthesis)
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Abstract: Pecan is an important horticultural nut crop originally from North America and now widely cultivated in China for its high ecological, ornamental and economic value. Currently, there are over one hundred cultivars grown in China, including introduced American cultivars and Chinese seedling breeding cultivars. Molecular markers were used to assess the genetic diversity of these cultivars and to identify the pedigrees of fine pecan plants with good characteristics and no cultivar-related data. A total of 77 samples grown in China were studied, including 14 introduced cultivars, 12 domestic seedling breeding cultivars, and 49 fine pecan plants with no cultivar data, together with Carya cathayensis and Juglans nigra. A total of 77 ISSR and 19 SSR primers were prescreened; 10 ISSR and eight SSR primers were selected, yielding a total of 94 amplified bands (100% polymorphic) in the range of 140–1,950 bp for the ISSR and 70 amplified bands (100% polymorphic) in the range of 50–350 bp for SSR markers. Genetic diversity analyses indicated Chinese-grown pecan cultivars and fine plants had significant diversity at the DNA level. The dengrograms constructed with ISSR, SSR or combined data were very similar, but showed very weak grouping association with morphological characters. However, the progeny were always grouped with the parents. The great diversity found among the Chinese cultivars and the interesting germplasm of the fine pecan plants analyzed in this study are very useful for increasing the diversity of the pecan gene pool. All 77 accessions in this study could be separated based on the ISSR and SSR fingerprints produced by one or more primers. The results of our study also showed that ISSR and SSR techniques were both suitable for genetic diversity analyses and the identification of pecan resources.
Keywords: genetic diversity; pecan; ISSR; SSR genetic diversity; pecan; ISSR; SSR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Jia, X.-D.; Wang, T.; Zhai, M.; Li, Y.-R.; Guo, Z.-R. Genetic Diversity and Identification of Chinese-Grown Pecan Using ISSR and SSR Markers. Molecules 2011, 16, 10078-10092.

AMA Style

Jia X-D, Wang T, Zhai M, Li Y-R, Guo Z-R. Genetic Diversity and Identification of Chinese-Grown Pecan Using ISSR and SSR Markers. Molecules. 2011; 16(12):10078-10092.

Chicago/Turabian Style

Jia, Xiao-Dong; Wang, Tao; Zhai, Min; Li, Yong-Rong; Guo, Zhong-Ren. 2011. "Genetic Diversity and Identification of Chinese-Grown Pecan Using ISSR and SSR Markers." Molecules 16, no. 12: 10078-10092.



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