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Molecules 2011, 16(12), 10029-10045; doi:10.3390/molecules161210029
Article

Extraction of Lepidium apetalum Seed Oil Using Supercritical Carbon Dioxide and Anti-Oxidant Activity of the Extracted Oil

1,†, 1,†, 1, 1,* , 1 and 2
1 Pharmacy College of Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China 2 Institute of Chemical Technology of Hua Chiao University, Xiamen 361021, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 5 September 2011 / Revised: 16 November 2011 / Accepted: 24 November 2011 / Published: 5 December 2011
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Abstract

The supercritical fluid extraction (SFE) of Lepidium apetalum seed oil and its anti-oxidant activity were studied. The SFE process was optimized using response surface methodology (RSM) with a central composite design (CCD). Independent variables, namely operating pressure, temperature, time and flow rate were evaluated. The maximum extraction of Lepidium apetalum seed oil by SFE-CO2 (about 36.3%) was obtained when SFE-CO2 extraction was carried out under the optimal conditions of 30.0 MPa of pressure, 70 °C of temperature, 120 min of extraction time and 25.95 L/h of flow rate. GC-MS analysis showed the presence of four fatty acids in Lepidium apetalum seed oil, with a high content (91.0%) of unsaturated fatty acid. The anti-oxidant activity of the oil was assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay and 2,2′-azino- bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) test. Lepidium apetalum seed oil possessed a notable concentration-dependent antioxidant activity, with IC50 values of 1.00 and 3.75 mg/mL, respectively.
Keywords: Lepidium apetalum; response surface methodology; supercritical fluid extraction; GC-MS; anti-oxidant activity Lepidium apetalum; response surface methodology; supercritical fluid extraction; GC-MS; anti-oxidant activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Xu, W.; Chu, K.; Li, H.; Chen, L.; Zhang, Y.; Tang, X. Extraction of Lepidium apetalum Seed Oil Using Supercritical Carbon Dioxide and Anti-Oxidant Activity of the Extracted Oil. Molecules 2011, 16, 10029-10045.

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