Molecules 2011, 16(12), 10046-10058; doi:10.3390/molecules161210046
Article

Changes in Morphology and Activity of Transglutaminase Following Cross-Linking and Immobilization on a Polypropylene Microporous Membrane

1, 1, 1, 1, 1, 2 and 1,* email
Received: 4 November 2011; in revised form: 28 November 2011 / Accepted: 30 November 2011 / Published: 5 December 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Transglutaminase (TGase) was cross-linked with glutaraldehyde, and cross-linked crystalline transglutaminase was immobilized on a polypropylene microporous membrane by UV-induced grafting. Immobilized enzyme activity were calculated to be 0.128 U/cm2 polypropylene microporous membrane. The microstructure and enzyme characteristics of free, cross-linked and immobilized transglutaminase were compared. The optimum temperature of free transglutaminase was determined to be approximately 40 °C, while cross-linking and immobilization resulted in an increase to approximately 45 °C and 50 °C. At 60 °C, immobilized, cross-linked and free transglutaminase retained 91.7 ± 1.20%, 63.2 ± 1.05% and 37.9 ± 0.98% maximum activity, respectively. The optimum pH was unaffected by the state of transglutaminase. However, the thermal and pH stabilities of cross-linked and immobilized transglutaminase were shown to increase.
Keywords: transglutaminase; cross-linking; immobilization; enzyme activity; microstructure
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MDPI and ACS Style

Shi, Y.-G.; Qian, L.; Zhang, N.; Han, C.-R.; Liu, Y.; Zhang, Y.-F.; Ma, Y.-Q. Changes in Morphology and Activity of Transglutaminase Following Cross-Linking and Immobilization on a Polypropylene Microporous Membrane. Molecules 2011, 16, 10046-10058.

AMA Style

Shi Y-G, Qian L, Zhang N, Han C-R, Liu Y, Zhang Y-F, Ma Y-Q. Changes in Morphology and Activity of Transglutaminase Following Cross-Linking and Immobilization on a Polypropylene Microporous Membrane. Molecules. 2011; 16(12):10046-10058.

Chicago/Turabian Style

Shi, Yan-Guo; Qian, Lei; Zhang, Na; Han, Chun-Ran; Liu, Ying; Zhang, Yi-Fang; Ma, Yong-Qiang. 2011. "Changes in Morphology and Activity of Transglutaminase Following Cross-Linking and Immobilization on a Polypropylene Microporous Membrane." Molecules 16, no. 12: 10046-10058.

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