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Molecules 2011, 16(11), 9480-9494; doi:10.3390/molecules16119480
Article

Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects

1,2, 1,* , 1, 1, 1 and 1
Received: 24 October 2011; in revised form: 7 November 2011 / Accepted: 8 November 2011 / Published: 14 November 2011
(This article belongs to the Section Metabolites)
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Abstract: The monocot lectin from the tubers of Arisaema erubescens (Wall.) Schott has been purified by consecutive hydrophobic chromatography and ion exchange chromatography methods. The molecular weight of this A. erubescens lectin (AEL) was determined to be about 12 kDa by high performance liquid chromatography (HPLC) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) methods. AEL could agglutinate rabbit erythrocytes. The haemagglutination activity of AEL was only inhibited by asialofetuin, while monosaccharide did not react. Rat paw edema and neutrophil migration models were used to investigate the pro-inflammatory activity of AEL. AEL (100 and 200 μg/paw) could induce significant rat paw edema. In addition, AEL (100, 200 and 300 μg/mL/cavity) could induce significant and dose-dependent neutrophil migration in the rat peritoneal cavities. Besides, AEL at doses ranging from 100 to 300 μg/mL/cavity could significantly increase the concentration of nitric oxide (NO), prostaglandin E2 (PGE2) and tumor necrosis factor alpha (TNF-α) in peritoneal fluid. As compared with control animals, 75% depletion in the number of resident cells following peritoneal lavage did not reduce the AEL-induced neutrophil migration. However, pre-treatment with 3% thioglycollate which increased the peritoneal macrophage population by 201%, enhanced the neutrophil migration induced by AEL (200 μg/mL/cavity) (p < 0.05). Reduction of peritoneal mast cell population by chronic treatment of rat peritoneal cavities with compound 48/80 (N-methyl-p-methoxyphenethylamine with formaldehyde) did not modify AEL-induced neutrophil migration. The results provided the basis for identifying the toxic components of A. erubescens and AEL could be a new useful tool for pro-inflammatory research.
Keywords: Arisaema erubescens lectin (AEL); haemagglutination activity; paw edema; neutrophil migration; pro-inflammatory compounds Arisaema erubescens lectin (AEL); haemagglutination activity; paw edema; neutrophil migration; pro-inflammatory compounds
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liu, X.Q.; Wu, H.; Yu, H.L.; Zhao, T.F.; Pan, Y.Z.; Shi, R.J. Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects. Molecules 2011, 16, 9480-9494.

AMA Style

Liu XQ, Wu H, Yu HL, Zhao TF, Pan YZ, Shi RJ. Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects. Molecules. 2011; 16(11):9480-9494.

Chicago/Turabian Style

Liu, Xian Qiong; Wu, Hao; Yu, Hong Li; Zhao, Teng Fei; Pan, Yao Zong; Shi, Run Jun. 2011. "Purification of a Lectin from Arisaema erubescens (Wall.) Schott and Its Pro-Inflammatory Effects." Molecules 16, no. 11: 9480-9494.



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