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Molecules 2011, 16(10), 8228-8243; doi:10.3390/molecules16108228
Article

SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway

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Received: 9 August 2011; in revised form: 20 September 2011 / Accepted: 22 September 2011 / Published: 28 September 2011
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Abstract: Typhonium giganteum Engl. (BaiFuzi) is one of the herbs commonly used in traditional Chinese medicine against cancer. In our previous studies, 37 compounds were identified the SFE-CO2 (supercritical fluid extraction with CO2) extract by GC-MS, including the four major components [β-sitosterol (40.22%), campesterol (18.45%), n-hexadecanoic acid (9.52%) and (Z,Z)-9,12-octadecadienoic acid (8.15%)]. The anti-cancer mechanisms of the SFE-CO2 extract from T. giganteum Engl. tubers have not been reported as yet. In this paper, the molecular mechanisms of the SFE-CO2 extract-mediated apoptosis in SMMC-7721 cells were further examined. SFE-CO2 extract inhibited the growth of SMMC-7721 cells in a time- and dose-dependent manner, arrested the cell cycle in the S phase and G2/M phase, and induced apoptosis. In addition, reactive oxygen species (ROS) increase, reduction of mitochondrial membrane potential, a rise in intracellular calcium levels were found in SMMC-7721 cells after treated with the extract. Western blot analysis showed that the extract caused down-regulation of Bcl-2 expression, and up-regulation of Bax expression. Moreover, caspase-3 and caspase-9 protease activity significantly increased in a dose-dependent manner. Collectively, our results showed that the SFE-CO2 extract from T. giganteum Engl. tubers induces apoptosis in SMMC-7721 cells involving a ROS-mediated mitochondrial signalling pathway.
Keywords: Typhonium giganteum Engl.; SFE-CO2 extract; SMMC-7721; Apoptosis; ROS Typhonium giganteum Engl.; SFE-CO2 extract; SMMC-7721; Apoptosis; ROS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, Q.; Jiang, C.; Zu, Y.; Song, Z.; Zhang, B.; Meng, X.; Qiu, W.; Zhang, L. SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway. Molecules 2011, 16, 8228-8243.

AMA Style

Li Q, Jiang C, Zu Y, Song Z, Zhang B, Meng X, Qiu W, Zhang L. SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway. Molecules. 2011; 16(10):8228-8243.

Chicago/Turabian Style

Li, Qingyong; Jiang, Chunfei; Zu, Yuangang; Song, Zhen; Zhang, Baoyou; Meng, Xiangdong; Qiu, Wei; Zhang, Li. 2011. "SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway." Molecules 16, no. 10: 8228-8243.


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