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Molecules 2011, 16(10), 8198-8208; doi:10.3390/molecules16108198
Article

HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents

1,* , 1
, 2
, 3
, 3
 and 1
1 Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovak Republic 2 Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovak Republic 3 Toxicological and Antidoping Centre, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovak Republic
* Author to whom correspondence should be addressed.
Received: 30 August 2011 / Revised: 15 September 2011 / Accepted: 20 September 2011 / Published: 28 September 2011
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Abstract

The aim of the study was a HPLC evaluation of the lipoxygenase activity inhibiting activity of a water infusion of Ligustrum vulgare L. leaves and selected isolates from it. The antiradical activity of the water infusion was determined using DPPH, ABTS and FRAP tests. Oleuropein and echinacoside concentrations in the water infusion were determined by HPLC. Water infusion, echinacoside and oleuropein were used for an antilipoxygenase activity assay using lipoxygenase isolated from rat lung cytosol fraction. Activity of 8-LOX, 12-LOX and 15-LOX were monitored through formation of 8-HETE, 12-HETE and 15-HETE, respectively. The water infusion exhibited the highest activity against all lipoxygenases, followed by oleuropein. Echinacoside was ineffective against LOXs in lower concentrations, while higher concentration showed similar inhibition on 8-LOX and 12-LOX. 15-LOX was affected more and the presence of echinacoside remarkably decreased its activity.
Keywords: Ligustrum; phenolics; inflammation; lipoxygenase; antioxidants; echinacoside; oleuropein Ligustrum; phenolics; inflammation; lipoxygenase; antioxidants; echinacoside; oleuropein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mučaji, P.; Záhradníková, A.; Bezáková, L.; Cupáková, M.; Rauová, D.; Nagy, M. HPLC Determination of Antilipoxygenase Activity of a Water Infusion of Ligustrum vulgare L. Leaves and Some of Its Constituents. Molecules 2011, 16, 8198-8208.

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