Molecules 2011, 16(1), 665-674; doi:10.3390/molecules16010665
Article

Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls

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Received: 16 December 2010; in revised form: 6 January 2011 / Accepted: 13 January 2011 / Published: 17 January 2011
(This article belongs to the Section Metabolites)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Tithonia diversifolia is a medicinal plant from the Municipality of Suchiapa, Chiapas, Mexico, that according to local folk medicine is considered useful in the treatment of gastric ulcers. The aim of the present study was to investigate the gastroprotective activity of T. diversifolia by using an ethanol-induced gastric ulcer experimental model in male Wistar rats. The results showed that T. diversifolia had gastroprotective activity, and that the dichloromethane extract had the highest protective activity (close to 90% when using doses between 10 to 100 mg/kg), and that further the compound tagitinin C isolated from this extract was the main active gastroprotective agent. Rats treated with tagitinin C suspended in Tween 80 at 1, 3, 10 and 30 mg/kg showed 37.7, 70.1, 100, and 100% gastroprotection, respectively. The effect elicited by tagitinin C (30 mg/kg) was not attenuated by pretreatment with either NG-nitro-L-arginine methyl ester (70 mg/kg, i.p.), a nitric oxide (NO) synthase inhibitor, N-ethylmaleimide (10 mg/kg, s.c.), a blocker of sulfhydryl groups, or indomethacin (10 mg/kg, s.c.), a blocker of prostaglandin synthesis, which suggests that the gastroprotective mechanism of action of this sesquiterpene lactone does not involve NO, sulfhydryl groups or prostaglandins.
Keywords: Tithonia diversifolia; Asteraceae; tagitinin C; gastroprotection; medicinal plants
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MDPI and ACS Style

Sánchez-Mendoza, M.E.; Reyes-Ramírez, A.; Cruz Antonio, L.; Martínez Jiménez, L.; Rodríguez-Silverio, J.; Arrieta, J. Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls. Molecules 2011, 16, 665-674.

AMA Style

Sánchez-Mendoza ME, Reyes-Ramírez A, Cruz Antonio L, Martínez Jiménez L, Rodríguez-Silverio J, Arrieta J. Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls. Molecules. 2011; 16(1):665-674.

Chicago/Turabian Style

Sánchez-Mendoza, María Elena; Reyes-Ramírez, Adelfo; Cruz Antonio, Leticia; Martínez Jiménez, Luis; Rodríguez-Silverio, Juan; Arrieta, Jesús. 2011. "Bioassay-Guided Isolation of an Anti-Ulcer Compound, Tagitinin C, from Tithonia diversifolia: Role of Nitric Oxide, Prostaglandins and Sulfhydryls." Molecules 16, no. 1: 665-674.

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