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Molecules 2010, 15(9), 6217-6230; doi:10.3390/molecules15096217

Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis

Jiangsu Key laboratory for TCM Formulae Research (Supported by the Cyrus Tang Foundation of USA); Nanjing University of Chinese Medicine, Nanjing 210046, China
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Received: 28 April 2010 / Revised: 16 August 2010 / Accepted: 19 August 2010 / Published: 3 September 2010
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Abstract

Shixiao San is a famous Traditional Chinese Medicine (TCM) formula that has been used for a long time for the treatment of gynecological diseases. In this paper, the active constituents in Shixiao San were characterized by using bioactivity evaluation followed by UPLC-QTOF and MarkerlynxTM analysis. The analgesic activities of two extracts of Shixiao San were evaluated using the hot-plate test, acetic acid-induced writhing and dysmenorrhea mice model. The results showed that the analgesic activity of Shixiao San vinegary extract (boiling vinegar) was superior to the aqueous extract. UPLC-QTOF and MarkerlynxTM analytic results showed that the process of boiling in vinegar may improve the dissolution of Shixiao San flavanoids, and these flavanoids may contribute to the observed analgesic activity. This work demonstrated that UPLC/QTOFMS and MarkerlynxTM could serve as new methods for fast generation and automated analysis of information-rich data from Chinese herbal medicines.
Keywords: Shixiao San; analgesic activity; active components; UPLC-QTOF-MS; MarkerlynxTM Shixiao San; analgesic activity; active components; UPLC-QTOF-MS; MarkerlynxTM
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MDPI and ACS Style

Zhou, W.; Su, S.-L.; Duan, J.-A.; Guo, J.-M.; Qian, D.-W.; Shang, E.-X.; Zhang, J. Characterization of the Active Constituents in Shixiao San Using Bioactivity Evaluation Followed by UPLC-QTOF and Markerlynx Analysis. Molecules 2010, 15, 6217-6230.

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