Molecules 2010, 15(6), 4452-4466; doi:10.3390/molecules15064452
Article

HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fraction of Orthosiphon Stamineus Leaves

1 School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia 2 Department of Anatomy, Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43000 Serdang, Selangor, Malaysia 3 Falkultas Farmasi, Universitas Sumatera Utara, 5 Jalan Almamater, USU-Kampus, Medan 20155, Indonesia
* Author to whom correspondence should be addressed.
Received: 13 April 2010; Accepted: 4 May 2010 / Published: 21 June 2010
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Abstract: The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three fractions using a dry flash column chromatography method. These three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammatory and nitric oxide (NO) inhibition using carrageenan-induced hind paw edema method. The flavonoid rich chloroform extract fraction (CF2) [containing sinensetin (2.86% w/w), eupatorin (5.05% w/w) and 3’-hydroxy-5,6,7,4’-tetramethoxyflavone (1.101% w/w)], significantly reduced rat hind paw edema, NO and decreased dye leakage to peritoneal cavity at p < 0.05. IC50 of in vitro NO scavenging of CF2 was 0.3 mg/mL. These results suggest that the anti-inflammatory properties of these CF2 may possibly be due to the presence of flavonoid compounds capable of affecting the NO pathway.
Keywords: anti-inflammatory activity; Orthosiphon stamineus; eupatorin; sinensetin; 3’-hydroxy-5,6,7,4’-tetramethoxyflavone; peritoneal capillary permeability

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MDPI and ACS Style

Yam, M.F.; Lim, V.; Salman, I.M.; Ameer, O.Z.; Ang, L.F.; Rosidah, N.; Abdulkarim, M.F.; Abdullah, G.Z.; Basir, R.; Sadikun, A.; Asmawi, M.Z. HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fraction of Orthosiphon Stamineus Leaves. Molecules 2010, 15, 4452-4466.

AMA Style

Yam MF, Lim V, Salman IM, Ameer OZ, Ang LF, Rosidah N, Abdulkarim MF, Abdullah GZ, Basir R, Sadikun A, Asmawi MZ. HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fraction of Orthosiphon Stamineus Leaves. Molecules. 2010; 15(6):4452-4466.

Chicago/Turabian Style

Yam, Mun Fei; Lim, Vuanghao; Salman, Ibrahim Muhammad; Ameer, Omar Ziad; Ang, Lee Fung; Rosidah, Noersal; Abdulkarim, Muthanna Fawzy; Abdullah, Ghassan Zuhair; Basir, Rusliza; Sadikun, Amirin; Asmawi, Mohd Zaini. 2010. "HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fraction of Orthosiphon Stamineus Leaves." Molecules 15, no. 6: 4452-4466.

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