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Molecules 2010, 15(6), 4452-4466; doi:10.3390/molecules15064452
Article

HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fraction of Orthosiphon Stamineus Leaves

1,2,* , 1, 1, 1, 1, 3
, 1, 1, 2
, 1
 and 1
1 School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia 2 Department of Anatomy, Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43000 Serdang, Selangor, Malaysia 3 Falkultas Farmasi, Universitas Sumatera Utara, 5 Jalan Almamater, USU-Kampus, Medan 20155, Indonesia
* Author to whom correspondence should be addressed.
Received: 13 April 2010 / Accepted: 4 May 2010 / Published: 21 June 2010
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Abstract

The aim of the present study was to verify the anti-inflammatory activity of Orthosiphon stamineus leaf extracts and to identify the active compound(s) contributing to its anti-inflammatory activity using a developed HPLC method. Active chloroform extract of O. stamineus was fractionated into three fractions using a dry flash column chromatography method. These three fractions were investigated for anti-peritoneal capillary permeability, in vitro nitric oxide scavenging activity, anti-inflammatory and nitric oxide (NO) inhibition using carrageenan-induced hind paw edema method. The flavonoid rich chloroform extract fraction (CF2) [containing sinensetin (2.86% w/w), eupatorin (5.05% w/w) and 3’-hydroxy-5,6,7,4’-tetramethoxyflavone (1.101% w/w)], significantly reduced rat hind paw edema, NO and decreased dye leakage to peritoneal cavity at p < 0.05. IC50 of in vitro NO scavenging of CF2 was 0.3 mg/mL. These results suggest that the anti-inflammatory properties of these CF2 may possibly be due to the presence of flavonoid compounds capable of affecting the NO pathway.
Keywords: anti-inflammatory activity; Orthosiphon stamineus; eupatorin; sinensetin; 3’-hydroxy-5,6,7,4’-tetramethoxyflavone; peritoneal capillary permeability anti-inflammatory activity; Orthosiphon stamineus; eupatorin; sinensetin; 3’-hydroxy-5,6,7,4’-tetramethoxyflavone; peritoneal capillary permeability
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yam, M.F.; Lim, V.; Salman, I.M.; Ameer, O.Z.; Ang, L.F.; Rosidah, N.; Abdulkarim, M.F.; Abdullah, G.Z.; Basir, R.; Sadikun, A.; Asmawi, M.Z. HPLC and Anti-Inflammatory Studies of the Flavonoid Rich Chloroform Extract Fraction of Orthosiphon Stamineus Leaves. Molecules 2010, 15, 4452-4466.

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