Abstract: Ultraviolet-B (UV-B) radiation is harmful to plants and human beings. Many secondary metabolites, like flavonoids, alkaloids, and lignin, are UV-B absorbing compounds, which can protect the genetic material of plants. Furthermore, they are active components of herbal drugs. UV-B radiation can activate the self-protective secondary metabolism system. The results of this paper provide a method to induce bioactive secondary metabolites from mulberry leaves (Morus alba L.) by UV-B irradiation in vitro. Five significantly different chromatographic peaks were found by HPLC fingerprint after induction, from which two active compounds were identified: One was chalcomoracin, a natural Diels-Alder type adduct with antibacterial activity; the other one was moracin N, which is a precursor of chalcomoracin. Their contents were 0.818 mg/g and 0.352 mg/g by dry weight, respectively.
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Gu, X.-D.; Sun, M.-Y.; Zhang, L.; Fu, H.-W.; Cui, L.; Chen, R.-Z.; Zhang, D.-W.; Tian, J.-K. UV-B Induced Changes in the Secondary Metabolites of Morus alba L. Leaves. Molecules 2010, 15, 2980-2993.
Gu X-D, Sun M-Y, Zhang L, Fu H-W, Cui L, Chen R-Z, Zhang D-W, Tian J-K. UV-B Induced Changes in the Secondary Metabolites of Morus alba L. Leaves. Molecules. 2010; 15(5):2980-2993.
Gu, Xi-Da; Sun, Ming-Yao; Zhang, Lin; Fu, Hong-Wei; Cui, Lei; Chen, Run-Ze; Zhang, Da-Wei; Tian, Jing-Kui. 2010. "UV-B Induced Changes in the Secondary Metabolites of Morus alba L. Leaves." Molecules 15, no. 5: 2980-2993.