Molecules 2010, 15(2), 680-688; doi:10.3390/molecules15020680
Article

A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry

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Received: 22 December 2009; in revised form: 9 January 2010 / Accepted: 29 January 2010 / Published: 29 January 2010
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.
Keywords: ochratoxin A; longan; fruit; pulp; solid-phase extraction; HPLC-FD; ESI-MS
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MDPI and ACS Style

Li, J.; Xie, H.; Yang, B.; Dong, X.; Feng, L.; Chen, F.; Jiang, Y. A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry. Molecules 2010, 15, 680-688.

AMA Style

Li J, Xie H, Yang B, Dong X, Feng L, Chen F, Jiang Y. A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry. Molecules. 2010; 15(2):680-688.

Chicago/Turabian Style

Li, Jing; Xie, Haihui; Yang, Bao; Dong, Xinhong; Feng, Linyan; Chen, Feng; Jiang, Yueming. 2010. "A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry." Molecules 15, no. 2: 680-688.

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