Molecules 2010, 15(2), 680-688; doi:10.3390/molecules15020680
Article

A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry

Jing Li 1 email, Haihui Xie 1 email, Bao Yang 1 email, Xinhong Dong 1 email, Linyan Feng 1 email, Feng Chen 2 email and Yueming Jiang 1,* email
1 South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China 2 Department of Food Science and Human Nutrition, Clemson University, Clemson, SC 29634, USA
* Author to whom correspondence should be addressed.
Received: 22 December 2009; in revised form: 9 January 2010 / Accepted: 29 January 2010 / Published: 29 January 2010
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Abstract: Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.
Keywords: ochratoxin A; longan; fruit; pulp; solid-phase extraction; HPLC-FD; ESI-MS

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MDPI and ACS Style

Li, J.; Xie, H.; Yang, B.; Dong, X.; Feng, L.; Chen, F.; Jiang, Y. A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry. Molecules 2010, 15, 680-688.

AMA Style

Li J, Xie H, Yang B, Dong X, Feng L, Chen F, Jiang Y. A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry. Molecules. 2010; 15(2):680-688.

Chicago/Turabian Style

Li, Jing; Xie, Haihui; Yang, Bao; Dong, Xinhong; Feng, Linyan; Chen, Feng; Jiang, Yueming. 2010. "A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry." Molecules 15, no. 2: 680-688.

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