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Molecules 2010, 15(2), 680-688; doi:10.3390/molecules15020680
Article

A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry

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1 South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China 2 Department of Food Science and Human Nutrition, Clemson University, Clemson, SC 29634, USA
* Author to whom correspondence should be addressed.
Received: 22 December 2009 / Revised: 9 January 2010 / Accepted: 29 January 2010 / Published: 29 January 2010
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Abstract

Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.
Keywords: ochratoxin A; longan; fruit; pulp; solid-phase extraction; HPLC-FD; ESI-MS ochratoxin A; longan; fruit; pulp; solid-phase extraction; HPLC-FD; ESI-MS
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Li, J.; Xie, H.; Yang, B.; Dong, X.; Feng, L.; Chen, F.; Jiang, Y. A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry. Molecules 2010, 15, 680-688.

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