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Molecules 2010, 15(11), 8518-8533; doi:10.3390/molecules15118518
Article

Characterization of Phosphate Solubilizing Bacteria in Sediments from a Shallow Eutrophic Lake and a Wetland: Isolation, Molecular Identification and Phosphorus Release Ability Determination

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Received: 12 September 2010; in revised form: 16 November 2010 / Accepted: 17 November 2010 / Published: 22 November 2010
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Abstract: The transformation of phosphorus (P) is a major factor of lake eutrophication, and phosphate releasing bacteria play an important role in the release process. Experiments were conducted to investigate P content and characterize phosphate solubilizing bacterial composition at the molecular level in a shallow eutrophic lake and a wetland. Results showed that P concentrations were relatively high and derived from agricultural runoff and domestic or industrial pollution. Enumeration and molecular identification of these strains indicated that these bacterial groups were abundant in the ecosystem and various kinds of bacteria participated in the phosphorus release process. Twelve phosphate solubilizing bacteria, including eight organic P-solubilizing bacteria (OPBs) and four inorganic P-solubilizing bacteria (IPBs), which belonged to three different families, were isolated and identified. Cupriavidus basilensis was found for the first time to have the ability to mineralize organic P (OP). Laboratory tests on P release ability revealed that IPBs were more effective at releasing P than OPBs. The most efficient IPB strain could accumulate over 170 mg·L-1 orthophosphate, while the equivalent OPB strain only liberated less than 4 mg·L-1 orthophosphate in liquid culture. The results obtained from this investigation should help clarify the roles of microorganisms in aquatic systems and the mechanisms of eutrophication.
Keywords: sediment; phosphorus; phosphate solubilizing bacteria; 16S rDNA; Cupriavidus basilensis sediment; phosphorus; phosphate solubilizing bacteria; 16S rDNA; Cupriavidus basilensis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Qian, Y.; Shi, J.; Chen, Y.; Lou, L.; Cui, X.; Cao, R.; Li, P.; Tang, J. Characterization of Phosphate Solubilizing Bacteria in Sediments from a Shallow Eutrophic Lake and a Wetland: Isolation, Molecular Identification and Phosphorus Release Ability Determination. Molecules 2010, 15, 8518-8533.

AMA Style

Qian Y, Shi J, Chen Y, Lou L, Cui X, Cao R, Li P, Tang J. Characterization of Phosphate Solubilizing Bacteria in Sediments from a Shallow Eutrophic Lake and a Wetland: Isolation, Molecular Identification and Phosphorus Release Ability Determination. Molecules. 2010; 15(11):8518-8533.

Chicago/Turabian Style

Qian, Yichao; Shi, Jiyan; Chen, Yingxu; Lou, Liping; Cui, Xinyi; Cao, Rukun; Li, Pengfei; Tang, Jie. 2010. "Characterization of Phosphate Solubilizing Bacteria in Sediments from a Shallow Eutrophic Lake and a Wetland: Isolation, Molecular Identification and Phosphorus Release Ability Determination." Molecules 15, no. 11: 8518-8533.



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