Molecules 2010, 15(11), 8060-8071; doi:10.3390/molecules15118060
Article

Chemopreventive Effects of Peucedanum praeruptorum DUNN and Its Major Constituents on SGC7901 Gastric Cancer Cells

Taigang Liang 1,2 email, Wenyan Yue 1 email and Qingshan Li 1,2,* email
1 School of Pharmaceutical Science, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China 2 School of Public Health, Shanxi Medical University, No 56, Xinjian Nan Road, Taiyuan 030001, Shanxi, China
* Author to whom correspondence should be addressed.
Received: 6 October 2010; in revised form: 1 November 2010 / Accepted: 3 November 2010 / Published: 9 November 2010
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Abstract: In this study, the effects of Peucedanum praeruptorum DUNN methanolic extract (PPME) and its major constituents on SGC7901 human gastric cancer cells were evaluated. Two pyranocoumarins, namely, (±) praeruptorin A (PA) and (±) praeruptorin B (PB), were isolated from PPME. A high performance liquid chromatographic (HPLC) method was developed to determine the contents of PA and PB in PPME. The antiproliferative and cytotoxic actions of PPME were observed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and release of lactate dehydrogenase (LDH) assays. At 300 µg/mL, PPME inhibited cell growth by 51.2% (P < 0.01), probably linked to the high concentration of PA and PB. Both PA and PB exhibited antiproliferative and cytotoxic activities on the SGC7901 cells. Furthermore, the active principle compound, PA, also enhanced the actions of doxorubincin (DOX) on SGC7901 cells. Cell growth decreased higher with the combined treatment of PA and DOX than that with the chemotherapy agent applied alone, suggesting that PA could reduce the dose of DOX for the desired effects.
Keywords: Peucedanum praeruptorum DUNN; (±) praeruptorin A; (±) praeruptorin B; chemopreventive effect; SGC7901 gastric cancer cell

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MDPI and ACS Style

Liang, T.; Yue, W.; Li, Q. Chemopreventive Effects of Peucedanum praeruptorum DUNN and Its Major Constituents on SGC7901 Gastric Cancer Cells. Molecules 2010, 15, 8060-8071.

AMA Style

Liang T, Yue W, Li Q. Chemopreventive Effects of Peucedanum praeruptorum DUNN and Its Major Constituents on SGC7901 Gastric Cancer Cells. Molecules. 2010; 15(11):8060-8071.

Chicago/Turabian Style

Liang, Taigang; Yue, Wenyan; Li, Qingshan. 2010. "Chemopreventive Effects of Peucedanum praeruptorum DUNN and Its Major Constituents on SGC7901 Gastric Cancer Cells." Molecules 15, no. 11: 8060-8071.

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