Next Article in Journal
Structural Necessity of Indole C5-O-Substitution of seco-Duocarmycin Analogs for Their Cytotoxic Activity
Previous Article in Journal
Oxidative Decarboxylation of Levulinic Acid by Cupric Oxides
Molecules 2010, 15(11), 7961-7970; doi:10.3390/molecules15117961
Article

Antimicrobial Metabolites from the Endophytic Fungus Pichia guilliermondii Isolated from Paris polyphylla var. yunnanensis

1, 1, 1, 1, 1,* , 2 and 3
1 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China 2 College of Science, China Agricultural University, Beijing 100193, China 3 College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China
* Author to whom correspondence should be addressed.
Received: 11 October 2010 / Revised: 3 November 2010 / Accepted: 4 November 2010 / Published: 5 November 2010
Download PDF [216 KB, uploaded 18 June 2014]

Abstract

Three steroids and one nordammarane triterpenoid were isolated for the first time from the endophytic fungus Pichia guilliermondii Ppf9 derived from the medicinal plant Paris polyphylla var. yunnanensis. By means of physicochemical and spectrometric analysis, they were identified as ergosta-5,7,22-trienol (1), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (2), ergosta-7,22-dien-3β,5α,6β-triol (3), and helvolic acid (4). Both micro-dilution-colorimetric and spore germination assays were employed to evaluate their antimicrobial activity. Among them, helvolic acid (4) exhibited the strongest antibacterial activity against all test bacteria, with MIC values ranging from 1.56 µg/mL to 50 µg/mL, and IC50 values from 0.98 µg/mL to 33.19 µg/mL. It also showed strong inhibitory activity on the spore germination of Magnaporthe oryzae with an IC50 value of 7.20 µg/mL. Among the three steroids, 5α,8α-epidioxyergosta-6,22-dien-3β-ol (2) exhibited relatively strong antimicrobial activity. The results suggest that the endophytic fungus Pichia guillermondii Ppf9 could be a candidate for producing helvolic acid, and the metabolites from this fungus could be potentially developed as antimicrobial agents in the future.
Keywords: endophytic fungus; Pichia guilliermondii; Paris polyphylla var. yunnanensis; steroids; helvolic acid; antimicrobial activity endophytic fungus; Pichia guilliermondii; Paris polyphylla var. yunnanensis; steroids; helvolic acid; antimicrobial activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Zhao, J.; Mou, Y.; Shan, T.; Li, Y.; Zhou, L.; Wang, M.; Wang, J. Antimicrobial Metabolites from the Endophytic Fungus Pichia guilliermondii Isolated from Paris polyphylla var. yunnanensis. Molecules 2010, 15, 7961-7970.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert