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Molecules 2010, 15(10), 7006-7015; doi:10.3390/molecules15107006

Analysis of the Chemical Composition of the Essential Oil of Polygonum minus Huds. Using Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GC-TOF MS)

1
Institute of Systems Biology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
2
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
3
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Received: 4 August 2010 / Revised: 8 October 2010 / Accepted: 10 October 2010 / Published: 12 October 2010
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Abstract

The essential oil in leaves of Polygonum minus Huds., a local aromatic plant, were identified by a pipeline of gas chromatography (GC) techniques coupled with mass-spectrometry (MS), flame ionization detector (FID) and two dimensional gas chromatography time of flight mass spectrometry (GC×GC–TOF MS). A total of 48 compounds with a good match and high probability values were identified using this technique. Meanwhile, 42 compounds were successfully identified in this study using GC-MS, a significantly larger number than in previous studies. GC-FID was used in determining the retention indices of chemical components in P. minus essential oil. The result also showed the efficiency and reliability were greatly improved when chemometric methods and retention indices were used in identification and quantification of chemical components in plant essential oil.
Keywords: comprehensive two-dimensional gas chromatography; volatile oil; TOF MS; Polygonum minus Huds; GC-MS; retention indices comprehensive two-dimensional gas chromatography; volatile oil; TOF MS; Polygonum minus Huds; GC-MS; retention indices
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Baharum, S.N.; Bunawan, H.; Ghani, M.A.; Mustapha, W.A.W.; Noor, N.M. Analysis of the Chemical Composition of the Essential Oil of Polygonum minus Huds. Using Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GC-TOF MS). Molecules 2010, 15, 7006-7015.

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