Next Article in Journal
Carnitine Conjugate of Nipecotic Acid: A New Example of Dual Prodrug
Previous Article in Journal
Protein Turnover in Mycobacterial Proteomics
Molecules 2009, 14(9), 3259-3267; doi:10.3390/molecules14093259
Communication

Antiproliferative, Cytotoxic, Antioxidant Activity and Polyphenols Contents in Leaves of Four Staphylea L. Species

1
, 1, 2
, 1, 1 and 3,*
1 Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojarov 10, 832 32 Bratislava, Slovakia 2 Department of Pharmaceutics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 612 42 Brno, Czech Republic 3 Institute of Experimental Endocrinology SAS, Vlarska 3, 833 06 Bratislava, Slovakia
* Author to whom correspondence should be addressed.
Received: 12 June 2009 / Revised: 13 August 2009 / Accepted: 26 August 2009 / Published: 28 August 2009
Download PDF [202 KB, uploaded 18 June 2014]

Abstract

Staphylea has been used for long time in Traditional Chinese Medicine (TCM) and by Native Americans in a number of therapeutical indications. The present study describes in vitro antiproliferative, cytotoxic properties (MTT and LDH test) and antioxidant activities (reduction of DPPH radical and peroxynitrite radical) of Staphylea colchica Stev. (SC), S. elegans Zab. (SC), S. holocarpa Hemsl. (SH) and S. pinnata L. (SP) leave water extracts. Time- (24 and 72 h) and dose- (1-150 μg/mL) dependent effects of the above extracts were tested at the mitochondrial (MTT test) and plasma membrane level (LDH leakage) in A431 human skin carcinoma cells. Screening of these properties has shown time and dose dependent increase of harmful effects, the highest activity was observed for the SE, while the less active was the SH extract. The ED50 values for the mitochondrial and membrane damage were nearly identical for the SE and very similar for SH extract. These findings indicate simultaneous injury of both cell compartments by SE and SH extracts. The highest antioxidant potential of SE species is accompanied by the highest content of flavones/flavonols and polyphenols. Only flavonoid contents are associated with antiproliferative effects and cell membrane injury, while antioxidant properties are the result of polyphenol content. The data clearly demonstrate that individual Staphylea L. species differ, not only in the amount of biologically active compounds, but also by the extent of harmful and beneficial effects.
Keywords: Staphylea; antiproliferative; cytotoxic; antioxidant Staphylea; antiproliferative; cytotoxic; antioxidant
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Lacikova, L.; Jancova, M.; Muselik, J.; Masterova, I.; Grancai, D.; Fickova, M. Antiproliferative, Cytotoxic, Antioxidant Activity and Polyphenols Contents in Leaves of Four Staphylea L. Species. Molecules 2009, 14, 3259-3267.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert