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Total Antioxidant Capacity of Some Commercial Fruit Juices: Electrochemical and Spectrophotometrical Approaches
Chemistry and Biochemistry Department, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine, bulevardul Marasti, no 59, Bucharest, Romania
Analytical Chemistry Department, Faculty of Chemistry, University of Bucharest, Panduri Avenue 90-92, code 050657, Bucharest, Romania
* Author to whom correspondence should be addressed.
Received: 26 November 2008; in revised form: 26 December 2008 / Accepted: 14 January 2009 / Published: 20 January 2009
Abstract: The aim of this paper was to assess the total antioxidant capacity of some commercial fruit juices (namely citrus), spectrophotometrically and by the biamperometric method, using the redox couple DPPH· (2,2-diphenyl-1-picrylhydrazyl)/DPPH (2,2-diphenyl-1-picrylhydrazine). Trolox® was chosen as a standard antioxidant. In the case of the spectrophometric method, the absorbance decrease of the DPPH· solution was followed. For the biamperometric method, the influence of some parameters like the potential diference, ΔE, DPPH· concentration, and Trolox® concentration was investigated. The calibration graph obtained for Trolox® presents linearity between 5 and 30 µM, (y = 0.059 x + 0.0564, where y represents the value of current intensity, expressed as μA and x the value of Trolox® concentration, expressed as μM; r2 = 0.9944). The R.S.D. value for the biamperometric method was 1.29% (n = 10, c = 15 μM Trolox®). In the case of the spectrophotometric method, the calibration graph obtained for Trolox® presents linearity between 0.01 and 0.125 mM (y = -9.5789 x+1.4533, where y represents the value of absorbance and x, the value of Trolox® concentration, expressed as mM; r2 = 0.9963). The R.S.D. value for the spectrophotometric method was 2.05%. Both methods were applied to total antioxidant activity determination in real samples (natural juices and soft drinks) and the results were in good agreement.
Keywords: Antioxidant activity; 2; 2-Diphenyl-1-picrylhydrazyl; 2; 2-Diphenyl-1-picrylhydrazine; Biamperometry; Pt electrode; Citrus juices
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MDPI and ACS Style
Pisoschi, A.M.; Cheregi, M.C.; Danet, A.F. Total Antioxidant Capacity of Some Commercial Fruit Juices: Electrochemical and Spectrophotometrical Approaches. Molecules 2009, 14, 480-493.
Pisoschi AM, Cheregi MC, Danet AF. Total Antioxidant Capacity of Some Commercial Fruit Juices: Electrochemical and Spectrophotometrical Approaches. Molecules. 2009; 14(1):480-493.
Pisoschi, Aurelia Magdalena; Cheregi, Mihaela Carmen; Danet, Andrei Florin. 2009. "Total Antioxidant Capacity of Some Commercial Fruit Juices: Electrochemical and Spectrophotometrical Approaches." Molecules 14, no. 1: 480-493.