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Molecules 2009, 14(1), 10-18; doi:10.3390/molecules14010010

A New Method for the Determination of Nucleic Acid Using an Eu3+– nicotinic Acid Complex as a Resonance Light Scattering Probe

Microscale Science Institute, Department of Chemistry, Weifang University, Weifang 261061, P.R. China
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China
Author to whom correspondence should be addressed.
Received: 24 November 2008 / Revised: 13 December 2008 / Accepted: 16 December 2008 / Published: 23 December 2008
(This article belongs to the Special Issue Nucleic Acids)
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This study found that in Tris-HCl buffer, the resonance light scattering (RLS) intensity of the Eu3+-nicotinic acid system can be greatly enhanced by nucleic acids and the enhanced intensity is proportional to the concentration of nucleic acid in the range of 7×10-8-1×10-5 g∙mL-1 for fsDNA, and its detection limit is 2×10-8 g∙mL-1. Based on this, a new method for the determination of nucleic acids is proposed. Synthetic and actual samples are determined satisfactorily. The interaction mechanism is also studied. It is thought that nucleic acid can bind with the Eu3+-nicotinic acid complex through electrostatic attraction and thus form a large Eu3+-nicotinic acid-nucleic acid complex. View Full-Text
Keywords: Nucleic acid; Nicotinic acid; Eu3+; Resonance light scattering Nucleic acid; Nicotinic acid; Eu3+; Resonance light scattering

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Guo, M.; Wang, L.-T.; Wu, X.; Xu, W.; Yang, J.-H. A New Method for the Determination of Nucleic Acid Using an Eu3+– nicotinic Acid Complex as a Resonance Light Scattering Probe. Molecules 2009, 14, 10-18.

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