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Molecules 2009, 14(1), 10-18; doi:10.3390/molecules14010010
Article

A New Method for the Determination of Nucleic Acid Using an Eu3+– nicotinic Acid Complex as a Resonance Light Scattering Probe

1,2
, 1, 2, 2 and 2,*
Received: 24 November 2008; in revised form: 13 December 2008 / Accepted: 16 December 2008 / Published: 23 December 2008
(This article belongs to the Special Issue Nucleic Acids)
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Abstract: This study found that in Tris-HCl buffer, the resonance light scattering (RLS) intensity of the Eu3+-nicotinic acid system can be greatly enhanced by nucleic acids and the enhanced intensity is proportional to the concentration of nucleic acid in the range of 7×10-8-1×10-5 g∙mL-1 for fsDNA, and its detection limit is 2×10-8 g∙mL-1. Based on this, a new method for the determination of nucleic acids is proposed. Synthetic and actual samples are determined satisfactorily. The interaction mechanism is also studied. It is thought that nucleic acid can bind with the Eu3+-nicotinic acid complex through electrostatic attraction and thus form a large Eu3+-nicotinic acid-nucleic acid complex.
Keywords: Nucleic acid; Nicotinic acid; Eu3+; Resonance light scattering Nucleic acid; Nicotinic acid; Eu3+; Resonance light scattering
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Guo, M.; Wang, L.-T.; Wu, X.; Xu, W.; Yang, J.-H. A New Method for the Determination of Nucleic Acid Using an Eu3+– nicotinic Acid Complex as a Resonance Light Scattering Probe. Molecules 2009, 14, 10-18.

AMA Style

Guo M, Wang L-T, Wu X, Xu W, Yang J-H. A New Method for the Determination of Nucleic Acid Using an Eu3+– nicotinic Acid Complex as a Resonance Light Scattering Probe. Molecules. 2009; 14(1):10-18.

Chicago/Turabian Style

Guo, Meng; Wang, Lin-Tong; Wu, Xia; Xu, Wei; Yang, Jing-He. 2009. "A New Method for the Determination of Nucleic Acid Using an Eu3+– nicotinic Acid Complex as a Resonance Light Scattering Probe." Molecules 14, no. 1: 10-18.


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