Next Article in Journal
Cholesterylbutyrate Solid Lipid Nanoparticles as a Butyric Acid Prodrug
Previous Article in Journal
Antifouling Metabolites from the Mangrove Plant Ceriops tagal
Article Menu

Export Article

Open AccessArticle
Molecules 2008, 13(2), 220-229; doi:10.3390/molecules13020220

Isolation and Identification of an Anti-tumor Component from Leaves of Impatiens balsamina

Faculty of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, P. R. China
College of Life Science, Zhejiang University, Hangzhou 310029, P. R. China
Author to whom correspondence should be addressed.
Received: 21 December 2007 / Revised: 18 January 2008 / Accepted: 18 January 2008 / Published: 31 January 2008
View Full-Text   |   Download PDF [237 KB, uploaded 18 June 2014]   |  


We have previously shown that ethanol or chloroform extracts of the leaves ofImpatiens balsamina (LIB) have anti-tumor activity against the human hepatocellularcarcinoma cell line HepG2. The ethanol extracts were separated into five fractionsaccording to polarity. An MTT assay indicated that two of the fractions had anti-tumoractivity and that the petroleum ether fraction (PEF) was the most active. But the availablequantities of both the PEF and chloroform fractions (CHF) were limited, precluding furtherstudy. The chloroform extract (CHE) shared almost all the same spots with the PEF andCHF and was plentiful enough to carry out further separations. Thus, the CHE was furtherseparated into six sub-fractions (CHE1~6) by column chromatography. A MTT assayshowed that only the CHE2 fraction had a strong tumor inhibition ratio (IC50 = 6.47±0.05mg/L), which was superior to that of curcumin (IC50 = 13.95±0.11 mg/L). However, TLCrevealed that CHE2 was not pure and still contained two more components. After furtherseparation and purification, followed by TLC and MTT assay confirmation, the final activecomponent was isolated and identified as 2-methoxy-1,4-naphthoquinone by m.p., UV, MSand 13C- and 1H-NMR data. This is the first report demonstrating that2-methoxy-1,4-naphthoquinone has intensive in vitro anti-tumor activity against HepG2cells.
Keywords: Impatiens balsamina; anti-tumor; MTT; TLC; 2-methoxy-1; 4-naphthoquinone Impatiens balsamina; anti-tumor; MTT; TLC; 2-methoxy-1; 4-naphthoquinone
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ding, Z.-S.; Jiang, F.-S.; Chen, N.-P.; Lv, G.-Y.; Zhu, C.-G. Isolation and Identification of an Anti-tumor Component from Leaves of Impatiens balsamina. Molecules 2008, 13, 220-229.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top