Next Article in Journal
Cholesterylbutyrate Solid Lipid Nanoparticles as a Butyric Acid Prodrug
Previous Article in Journal
Antifouling Metabolites from the Mangrove Plant Ceriops tagal
Molecules 2008, 13(2), 220-229; doi:10.3390/molecules13020220

Isolation and Identification of an Anti-tumor Component from Leaves of Impatiens balsamina

1 Faculty of Life Science, Zhejiang Chinese Medical University, Hangzhou, 310053, P. R. China 2 College of Life Science, Zhejiang University, Hangzhou 310029, P. R. China
* Author to whom correspondence should be addressed.
Received: 21 December 2007 / Revised: 18 January 2008 / Accepted: 18 January 2008 / Published: 31 January 2008
View Full-Text   |   Download PDF [237 KB, uploaded 18 June 2014]   |   Browse Figures


We have previously shown that ethanol or chloroform extracts of the leaves ofImpatiens balsamina (LIB) have anti-tumor activity against the human hepatocellularcarcinoma cell line HepG2. The ethanol extracts were separated into five fractionsaccording to polarity. An MTT assay indicated that two of the fractions had anti-tumoractivity and that the petroleum ether fraction (PEF) was the most active. But the availablequantities of both the PEF and chloroform fractions (CHF) were limited, precluding furtherstudy. The chloroform extract (CHE) shared almost all the same spots with the PEF andCHF and was plentiful enough to carry out further separations. Thus, the CHE was furtherseparated into six sub-fractions (CHE1~6) by column chromatography. A MTT assayshowed that only the CHE2 fraction had a strong tumor inhibition ratio (IC50 = 6.47±0.05mg/L), which was superior to that of curcumin (IC50 = 13.95±0.11 mg/L). However, TLCrevealed that CHE2 was not pure and still contained two more components. After furtherseparation and purification, followed by TLC and MTT assay confirmation, the final activecomponent was isolated and identified as 2-methoxy-1,4-naphthoquinone by m.p., UV, MSand 13C- and 1H-NMR data. This is the first report demonstrating that2-methoxy-1,4-naphthoquinone has intensive in vitro anti-tumor activity against HepG2cells.
Keywords: Impatiens balsamina; anti-tumor; MTT; TLC; 2-methoxy-1; 4-naphthoquinone Impatiens balsamina; anti-tumor; MTT; TLC; 2-methoxy-1; 4-naphthoquinone
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Ding, Z.-S.; Jiang, F.-S.; Chen, N.-P.; Lv, G.-Y.; Zhu, C.-G. Isolation and Identification of an Anti-tumor Component from Leaves of Impatiens balsamina. Molecules 2008, 13, 220-229.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert