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Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides
Department of Pharmacy, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022. P. R. China
School of Pharmacy, Anhui Medical University, Hefei, 230032. P. R. China
* Author to whom correspondence should be addressed.
Received: 22 August 2008; in revised form: 24 September 2008 / Accepted: 26 September 2008 / Published: 1 October 2008
Abstract: Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH2 column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.
Keywords: Astragalus polysaccharides; Isolation and Purification; Molecular Weight; Monosaccharide composition
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Xu, D.-J.; Xia, Q.; Wang, J.-J.; Wang, P.-P. Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides. Molecules 2008, 13, 2408-2415.
Xu D-J, Xia Q, Wang J-J, Wang P-P. Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides. Molecules. 2008; 13(10):2408-2415.
Xu, Du-Juan; Xia, Quan; Wang, Jia-Jia; Wang, Pei-Pei. 2008. "Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides." Molecules 13, no. 10: 2408-2415.