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Molecules 2008, 13(10), 2408-2415; doi:10.3390/molecules13102408
Article

Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides

1,* , 1, 2 and 2
1 Department of Pharmacy, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022. P. R. China 2 School of Pharmacy, Anhui Medical University, Hefei, 230032. P. R. China
* Author to whom correspondence should be addressed.
Received: 22 August 2008 / Revised: 24 September 2008 / Accepted: 26 September 2008 / Published: 1 October 2008
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Abstract

Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH2 column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.
Keywords: Astragalus polysaccharides; Isolation and Purification; Molecular Weight; Monosaccharide composition Astragalus polysaccharides; Isolation and Purification; Molecular Weight; Monosaccharide composition
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Xu, D.-J.; Xia, Q.; Wang, J.-J.; Wang, P.-P. Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides. Molecules 2008, 13, 2408-2415.

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