A Standard Addition Method to Assay the Concentration of Biologically Interesting Polyphenols in Grape Berries by Reversed-Phase HPLC

doi:10.3390/12092259

This article is

freely available
re-usable

Molecules 2007, 12(9), 2259-2269; doi:10.3390/12092259

Article

A Standard Addition Method to Assay the Concentration of Biologically Interesting Polyphenols in Grape Berries by Reversed-Phase HPLC

Aphrodite-Victoria Sakkiadi, Constantino A. Georgiou and Serkos A. Haroutounian*

Chemistry Laboratory, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece

* Author to whom correspondence should be addressed.

Received: 19 September 2007; in revised form: 26 September 2007 / Accepted: 26 September 2007 / Published: 27 September 2007

Download PDF Full-Text [99 KB, uploaded 2 October 2008 11:53 CEST]

Abstract: A reversed-phase HPLC method which allows the simultaneous assay of (+)- catechin, (–)-epicatechin, trans-resveratrol, quercetin and quercetin glycosides in grape berries is described. Kromasil 100 served as stationary phase and a gradient of acetic acid, water and methanol was used. The analytical run requires 42 min for complete sample elution. Satisfactory peak resolution was achieved following a novel extraction process and direct injection of a 20 μL sample. The method was used for the analyses of eighteen samples. Linearities were in the range of 0.98 to 0.999 regression coefficient, for all phenolics, while detection limits ranged from 30 μg mL–1 for trans-resveratrol to 1.5 mg mL–1 for (+)-catechin. Recoveries ranged from 95.1 to 98.7% while the method provided good precision, with standard deviations between 3.5 and 6.1%, n=5.

Keywords: Vitis vinifera; grape berries; polyphenols; flavonoids

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Sakkiadi, A.-V.; Georgiou, C.A.; Haroutounian, S.A. A Standard Addition Method to Assay the Concentration of Biologically Interesting Polyphenols in Grape Berries by Reversed-Phase HPLC. Molecules 2007, 12, 2259-2269.

AMA Style

Sakkiadi A-V, Georgiou CA, Haroutounian SA. A Standard Addition Method to Assay the Concentration of Biologically Interesting Polyphenols in Grape Berries by Reversed-Phase HPLC. Molecules. 2007; 12(9):2259-2269.

Chicago/Turabian Style

Sakkiadi, Aphrodite-Victoria; Georgiou, Constantino A.; Haroutounian, Serkos A. 2007. "A Standard Addition Method to Assay the Concentration of Biologically Interesting Polyphenols in Grape Berries by Reversed-Phase HPLC." Molecules 12, no. 9: 2259-2269.

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert