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Molecules 2007, 12(3), 552-562; doi:10.3390/12030552

Preparative Separation and Identification of the Flavonoid Phlorhizin from the Crude Extract of Lithocarpus Polystachyus Rehd

1,* , 1
1 College of Light Industry and Food Technology, South China University of Technology, Guangzhou, 510641, P.R. China 2 Department of Food Science, Foshan University, Nanhai Dali, Guangdong 528231, P.R. China
* Author to whom correspondence should be addressed.
Received: 6 February 2007 / Revised: 13 March 2007 / Accepted: 13 March 2007 / Published: 20 March 2007
(This article belongs to the Special Issue Phenolics and Polyphenolics)
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The flavonoid phlorhizin is abundant in the leaves of Sweet Tea(ST, Lithocarpus Polystachyus Rehd). Phlorhizinwas preparatively separated and purified from a crude ST extract containing 40% total flavonoids by static adsorption and dynamic desorption on ADS-7 macroporous resin and neutral alumina column chromatography. Only water and ethanol were used as solvents and eluants throughout the whole separation and purification process. Using a phlorhizin standard as the reference compound, the target compound separated from the crude ST extracts was analyzed by thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and electrosprayionization mass spectrometry (EIS-MS) and identified as 99.87% pure (by HPLC-UV) phlorhizin. The results showed that 10g of the targetcompound could be obtained from 40g of the crude extracts in a single operation, indicating a 40% recovery. Therefore, this represents an efficientand environmentally-friendly technology for separating and purifying phlorhizinfrom ST leaves.
Keywords: Phlorhizin; separation; Lithocarpus polystachyus; macroporous resin Phlorhizin; separation; Lithocarpus polystachyus; macroporous resin
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dong, H.; Ning, Z.; Yu, L.; Li, L.; Lin, L.; Huang, J. Preparative Separation and Identification of the Flavonoid Phlorhizin from the Crude Extract of Lithocarpus Polystachyus Rehd. Molecules 2007, 12, 552-562.

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