- freely available
- re-usable

*Entropy*
**2014**,
*16*(1),
41-61;
doi:10.3390/e16010041

^{1}

^{2}

Published: 27 December 2013

## Abstract

**:**As shown by Jarzynski, free energy differences between equilibrium states can be expressed in terms of the statistics of work carried out on a system during non-equilibrium transformations. This exact result, as well as the related Crooks fluctuation theorem, provide the basis for the computation of free energy differences from fast switching molecular dynamics simulations, in which an external parameter is changed at a finite rate, driving the system away from equilibrium. In this article, we first briefly review the Jarzynski identity and the Crooks fluctuation theorem and then survey various algorithms building on these relations. We pay particular attention to the statistical efficiency of these methods and discuss practical issues arising in their implementation and the analysis of the results.

## 1. Introduction

The calculation of free energies from atomistic simulations is of great importance in many applications, ranging from the prediction of the phase behavior of a certain substance to the calculation of ligand affinities in drug design. Since the computation of free energies (or, more precisely, of free energy differences) involves the determination of entropic contributions and, hence, the estimation of phase space volumes [1], free energy calculations are computationally very demanding in most cases. Therefore, a significant effort has been devoted to the development of more efficient free energy calculation algorithms. This endeavor has received new momentum with Jarzynski’s discovery of a very general relation between equilibrium free energies and non-equilibrium work [2,3], which has inspired several molecular dynamics-based algorithms for free energy computations. In this article, we will give an overview of these methods.

According to the maximum work theorem, a consequence of the second law of thermodynamics, the amount of work W performed on a system during a non-equilibrium transformation is larger than the free energy difference ΔF between the equilibrium states corresponding to the transition end points:

Equivalently, the amount of work that can be extracted from a system is bounded from above by the free energy difference. In the above equation, the equal sign holds only if the transformation is carried out reversibly, maintaining equilibrium at all times. The angular brackets on the left-hand side of the maximum work theorem indicate an average over many realizations of the non-equilibrium process. If one considers a macroscopic system, for instance, a piston compressing a gas enclosed in a cylinder, the average is not necessary, because every realization of the process yields, for all practical purposes, the same amount of work W, if the transformation is carried out following the same protocol. This is essentially a consequence of the central limit theorem for thermal fluctuations. In the case of a microscopic system, however, fluctuations become important, and different realizations of the transformation typically produce different work values, leading to a statistical distribution of W. For instance, stretching a biomolecule with atomic force microscopes or optical tweezers will cost a different amount work for each repetition of the experiment. In some cases, the work expended on the system might even be smaller than the free energy difference, seemingly violating the maximum work theorem and, hence, the second law of thermodynamics.

As shown by Jarzynski in 1997 [2,3], the work fluctuations resulting for microscopic systems can be accounted for in an exact way, transforming the maximum work theorem into an equality:

_{B}T is the reciprocal temperature of the equilibrium state from which the transformation is started, and k

_{B}is the Boltzmann constant. Remarkably, this result, now commonly referred to as Jarzynski equation or Jarzynski non-equilibrium work theorem, relates the statistics of irreversible work carried out on the system, while it is driven away from equilibrium, to an equilibrium free energy difference. A closely connected result is the Crooks fluctuation theorem [4–6], which relates the equilibrium free energy difference to the work distributions of the forward and reversed process.

In general, processes during which work is performed on or by the system drive the system away from equilibrium, such that the phase space distribution obtained at the end of the process may differ strongly from the equilibrium distribution to which the system relaxes after the external perturbation has been stopped. For instance, a piston pushed quickly into a gas-filled cylinder generates non-equilibrium states with strong flows markedly different from the static equilibrium state to which the gas eventually relaxes after the piston has reached its final state. At first sight, it is therefore surprising that equilibrium properties, such as free energy differences, can be extracted from non-equilibrium trajectories. As discussed in the following sections of this paper, a closer analysis reveals that averaging over the work exponential is equivalent to removing the bias introduced during the driving process. It is this unbiasing that ultimately permits the extraction of equilibrium properties (as we will discuss in Section 5, in principle, one can determine the entire equilibrium distribution and not only the free energy) from non-equilibrium trajectories. Thus, the non-equilibrium work theorem can be viewed as a prescription of how to compensate for the effects of manipulations that drive the system into non-equilibrium rather than a tool that illuminates the nature of non-equilibrium processes. Nevertheless, it is remarkable that the bias has a very simple exponential form and can be expressed in terms of the work only.

The Jarzynski non-equilibrium work theorem, as well as the Crooks fluctuation theorem provide the framework for the interpretation of single-molecule pulling experiments [7–9], in which non-equilibrium effects can never be fully avoided. These exact results can also be exploited to devise computer simulation algorithms for the calculation of free energies. In this article, we review several computational approaches based on the collection of work statistics in a fast-switching non-equilibrium setting, paying particular attention to the accuracy and efficient implementation of these methods compared to conventional free energy computation methods (see [10–12]). In the remainder of this article, we will first state the Jarzynski and Crooks theorems more explicitly and discuss the conditions under which they apply. After that, we will survey several fast switching algorithms in which free energies are determined from sets of molecular dynamics trajectories obtained while changing a control parameter, thereby exerting work on the system. We conclude with a brief summary and outlook to future possibilities and applications.

## 2. Jarzynski Identity and Crooks Fluctuation Theorem

To set the notation, consider a classical system with energy H(x, λ) depending on the microscopic state x of the system, as well as on a parameter λ. The microscopic state x is specified by the positions of all particles in the system and, if necessary, also by all momenta. The parameter λ is a control parameter that can be changed externally, for instance, the volume of the cylinder containing the particles or an external field. According to the basic laws of statistical mechanics, the free energy difference between the two equilibrium states A and B corresponding to the values λ_{A} and λ_{B}, respectively, of the order parameter is given by:

_{A}= ∫ dx exp{−βH(x, λ

_{A})} and Z

_{B}= ∫ dx exp{−βH(x, λ

_{B})} are the canonical partition functions of the two equilibrium states (up to a combinatorial prefactor irrelevant for our considerations). The free energy difference ΔF is the work required to change the external parameter from λ

_{A}to λ

_{B}in a reversible process. Such a reversible transformation could be realized, for instance, by changing the parameter λ infinitely slowly, while keeping the system in contact with a heat bath. In this case, the free energy difference is equal to the work of the system.

Instead of changing the control parameter λ very slowly, one could change it at a finite rate over a time interval τ, following a certain protocol λ(t), where λ(0) = λ_{A} and λ(τ) = λ_{B}. In general, such a fast switching of the control parameter drives the system away from equilibrium in an irreversible way, such that the work required to do the change exceeds the free energy difference, as posited by the maximum work theorem of Equation (1). To be more specific, the work performed on the system along a particular trajectory x(t) is the energy change caused by changes of the control parameter accumulated along the trajectory:

_{A})) for control parameter λ

_{A}. In a computer simulation, one could realize such a process by sampling initial conditions from a canonical distribution and then integrating the underlying equations of motion, while at the same time changing the control parameter λ according to the protocol λ(t).

Jarzynski has shown [2,3] that averaging over the exponential of the work exp(−βW (τ)) rather than the work, turns the maximum work theorem into an equality, 〈exp{−βW[x(t), λ(t)]}〉 = exp{− βΔF}. It is important to realize that the average over the work exponential involves two averages, one over the distribution of initial conditions and another one over the set of trajectories that originate from a particle initial condition. For deterministic dynamics, the initial condition determines the entire trajectory, x(t), but for stochastic dynamics, the system evolves in different ways, even if one repeatedly starts from the same initial condition. Hence, for stochastic dynamics, the average appearing in the Jarzynski equation also requires an average over noise histories.

The Jarzynski equation is an exact result that holds under very general conditions. The requirements are that initially, the system must be in equilibrium and that for a fixed control parameter, the dynamics conserves the equilibrium distribution corresponding to that value of the control parameter. The latter condition is satisfied by most types of dynamics usually used in computer simulations, including Newtonian, thermostated, Langevin and Monte Carlo dynamics. It is worth pointing out that it is not necessary that the system be in an equilibrium state at the end of the transformation process or relax towards equilibrium after the control parameter switching is completed. Furthermore, it is interesting that the Jarzynski equation holds, even if the switching is carried out according to different (though prescribed) protocols provided that λ(0) = λ_{A} and λ(τ) = λ_{B}, i.e., all protocols start at λ_{A} at time 0 and finish at λ_{B} at time τ. After Jarzynski’s seminal work [2], in which the Jarzynski equality was derived for systems evolving deterministically with and without coupling to a heat bath, several other proofs were provided, for instance, based on a master equation [3], for Markovian dynamics satisfying detailed balance [5,13], for dynamical systems conserving the canonical distribution [14] or from the Feynman–Kac theorem [7].

In the limiting cases of infinitely fast switching and infinitely slow switching, the Jarzynski equality reduced to two well-known results. For instantaneous switching, τ → 0, the initial and final point of a trajectory are identical, as the system has no time to evolve. In this case, the work carried out on the system at a particular microscopic state x equals the difference in energy evaluated for the two values of the control parameter:

_{A}. The above equation is the central result of free energy perturbation theory [15] and is often used to compute free energy differences. In the opposite limit of infinitely slow switching, τ → ∞, the system has time to equilibrate for every intermediate value of the control parameter, such that the Jarzynski equation together with Equation (4) implies:

_{λ}. The free energy difference is then obtained by numerical integration, for instance, by using the Simpson rule or more sophisticated integration schemes. The maximum work theorem of Equation (1) also immediately follows from the Jarzynski equation by virtue of Jensen’s inequality, 〈exp(−x) 〉 ≥ exp(−〈x〉).

As mentioned in the introduction, the Jarzynski equation can be viewed as a way to remove the bias introduced by the switching process into the phase space distribution obtained at the end of the process. Following similar considerations as those used to derive the Jarzynski equality, one can prove that for any phase space function A(x) the following equation holds [4,7,17]:

_{B}, and the average on the right-hand side is an average over non-equilibrium pathways generated with protocol λ(t) just as in the Jarzynski equations. To make this difference even more explicit, we have added the subscripts eq and non-eq to the equilibrium and non-equilibrium average, respectively. In the above equation, x(τ) refers to the endpoints of the non-equilibrium trajectories. The Jarzynski equation is simply obtained by setting A(x) = 1. Equation (8) implies that equilibrium averages can be computed by reweighting the non-equilibrium distribution obtained as a result of the switching procedure by exp(−βW + βΔF). In particular, the equilibrium distribution for λ

_{B}is obtained by setting A(x) = δ(x − x(τ)), where δ(x) is the Dirac delta function:

_{B}(and with appropriate modifications, also for all intermediate values λ(t) of the control parameter) can be extracted from a set of non-equilibrium trajectories obtained from simulation or experiment.

If the dynamics of the system not only conserves the equilibrium distribution for a fixed control parameter, but is also microscopically reversible, i.e., if it satisfies detailed balance, the work distribution for the forward process is simply related to that of the process carried out with the time reversed protocol. More specifically, the distribution P(W) of work W observed in repeated realizations of the switching process is given by:

_{R}(t) = λ(τ − t). The distribution P

_{R}(W), observed for the reverse process, in which the control parameter is changed from λ

_{B}back to λ

_{A}, can be written as:

_{R}(t). Crooks has shown that for dynamics that is microscopically reversible, the work distributions P(W) and P

_{R}(W) for the forward and reverse process, respectively, are related by [5,6]:

## 3. Implementing Fast Switching Simulations

Jarzynski’s non-equilibrium work theorem and the Crooks fluctuation theorem suggest interesting algorithms for the calculation of free energy differences. The power of these algorithms derives from the fact that all quantities appearing in these relations can be easily determined. The simplest of these algorithms consists in the following steps. First, one needs to prepare initial conditions distributed according to the Boltzmann–Gibbs distribution. This can be achieved using a variety of methods, for instance, canonical Monte Carlo simulation, possibly combined with enhanced sampling methods, such as parallel replica sampling, or with thermostated molecular dynamics. To improve the efficiency of the free energy calculation, it is important to make sure that these initial conditions are sufficiently decorrelated.

From these initial conditions, one then starts trajectories of the desired length that are integrated, while, at the same time, changing the control parameter according to the protocol λ(t). Both the choice of the parameter λ used to drive the transformation, as well as the shape of the protocol influence the efficiency of the calculation, as described in detail below. One can compute the dynamics of the system based on stochastic equations of motion, such as the Langevin equation, or deterministic equations of motion, such as Newton’s equations with or without thermostat. Along the computed trajectories, one then has to compute the work W carried out on the system by changing the control parameter. This is most easily done by dividing the basic molecular dynamics steps into two sub-steps. In the first sub-step, the state x(t + Δt) of the system at time t + Δt is computed by carrying out an integration step with the control parameter fixed at value λ(t). In the second sub-step, one then changes the control parameter from λ(t) to λ(t + Δt), while keeping the state x(t + Δt) of the system unchanged. Only in this second sup-step is work carried out on the system. In this two-step procedure, the work carried out on the system along a particular trajectory up to time t + Δt is given by:

_{t}≡ x(t) and λ

_{t}≡ λ(t) are the state of the system and the value of the control parameter at time t, respectively. From the work values collected in this way for the forward process, and possibly also for the backward process, one can then determine the free energy difference by applying the types of analyses discussed in the next section.

An important choice one has to make in the context of fast switching free energy computations is how to allocate computing time. In particular, one has to decide whether to generate many short trajectories with a large switching rate or fewer and longer trajectories along which the system is driven more gently. Without enhanced sampling schemes, as those discussed in subsequent sections, one generally expects the slow switching regime to give more accurate free energy estimates for a given amount of computing time [18]. As a rule of thumb, one should carry out the switching slowly enough, such that the standard deviation of the work values does not exceed k_{B}T. In this slow switching regime, the statistical error obtained with a given amount of computing time grows slowly with the switching rate. It is nevertheless more advantageous to compute several trajectories at a moderate switching rate than one single long trajectory, because then, an error estimate for the free energy can be obtained in a straightforward manner. Furthermore, multiple trajectories can be run in parallel to exploit the capabilities of parallel processing machines. Another important choice to make in fast switching simulations concerns the direction in which the transformation is carried out. Interestingly, it can be shown that the direction in which more work is dissipated is computationally beneficial [19]. This formal result is consistent with experience in free energy calculations using perturbation theory. In the calculation of chemical potentials, for instance, test particle insertion typically produces a larger variation in the energy change compared to particle removal and leads to more accurate estimates of the chemical potential [1].

As discussed above, the statistical error of a free energy computed via fast switching strongly depends on the rate at which the system is driven out of equilibrium. However, while the switching rate is certainly the most important parameter, also the particular shape of the protocol λ(t) for a given total switching time τ plays an important role in determining the accuracy of the free energy estimate. Since the Jarzynski equality and the Crooks fluctuation theorem hold for arbitrary protocols, one can exploit this freedom to design protocols that optimize the free energy computation. Recently, Schmiedl and Seifert have addressed a related question, asking how the protocol should be designed to minimize the average work expended during the non-equilibrium transformation for a given total of τ [20]. Their analysis, carried out for a particle dragged through a fluid and for a particle in a harmonic trap with changing strength, indicates that, surprisingly, the optimum protocol has discontinuous jumps, both at the beginning and at the end of the process. This result is in contrast to an earlier linear-response analysis [21], which implied that the optimum protocol is smooth and free of jumps. In the cases studied by Schmiedl and Seifert, the optimum protocol with jumps led to a reduction of the dissipated work by up to 12% compared to the case with a continuous protocol changing linearly in time. A subsequent numerical study of a non-linear system carried out by Then and Engel [22] showed that the optimum protocol can have one, two or even more jumps. Steps occur also in the optimum protocol for underdamped Langevin dynamics, for which also delta-like singularities appear at the start and the end of the switching process, effectively kicking the system discontinuously [23].

While, in general, protocols in which the dissipated work is small are expected to yield a more accurate free energy estimate, there is no simple relation between the average work and the statistical error in the free energy. Hence, a protocol optimized with respect to the work does not necessarily minimize the statistical error. However, numerical protocol optimizations conducted for various models indicate that control parameter steps at the start and the end of the protocol (but never in between) are beneficial also for free energy computations [24]. These steps are most pronounced in the fast switching regime and disappear for slow switching. For small switching rates, the minimum work protocol and the minimum error protocol are identical, but for large switching rates, that may differ. In some cases the minimum error protocol even yields an average work that is larger than that of a linear protocol without steps. While appropriate steps in the protocol can lead to a considerable reduction of the computational cost of fast switching free energy calculations, such large savings typically occur only in switching regimes where the straightforward application of the Jarzynski equality is impractical. Whether work biased sampling schemes (discussed in Section 6) may serve to leverage the potential power of discontinuous protocols is currently an open question.

## 4. Analysis of Non-Equilibrium Free Energy Calculations

The simplest, but also most error-prone, method to obtain free energies from one-sided non-equilibrium simulations is a direct evaluation of the exponential estimator:

_{i}are the work values obtained in n independent non-equilibrium runs. If the work distribution is broad, with a variance var(W) ≫ (k

_{B}T)

^{2}, then the estimate will tend to be dominated by only a few trajectories [19]. All others have negligible weight, resulting not only in sampling inefficiency, but also a systematic bias of the free energy estimate (i.e., the average of ΔF, obtained in repeated sampling with a fixed number n of trajectories, deviates from the exact value [25]). The resulting systematic errors can be estimated and at least partly corrected [17,26–28]. Alternatively, the width of the work distribution can be reduced by breaking the transformation up into segments [18,29,30]. However, the computational cost of re-equilibration at intermediate stages can be significant. The bias can also be eliminated by using cumulant estimators [2,18], in particular, the second-order approximation:

More accurate and asymptotically unbiased free energy estimates can be obtained from two-sided simulations by using the Crooks relation. By exploiting the analogy between equilibrium perturbation theory and non-equilibrium simulations, one can adapt Bennett’s acceptance ratio as the estimator [33,34]. It requires solving an implicit relation:

_{i}and W̲

_{i}are the work values obtained on the n

_{f}and n

_{b}forward and reverse transformations, respectively. This equation can be solved numerically, e.g., by using the Newton–Raphson method. Note that the work values, W̲

_{i}, on the reversed path have the opposite sign.

The analogy to the equilibrium method also allows us to adapt two-sided cumulant estimators [35] to non-equilibrium work distributions [18] or to use Bennett’s overlapping histogram method [33]. While less efficient as a free energy estimator than the acceptance ratio method, the histogram method provides us with a test of consistency between forward and reverse transformations. According to Equation (12), a plot of the logarithm of P(W)/P_{R}(−W) should be a straight line as a function of W with slope β. Deviations point to sampling issues or other problems. Another approach [36] for the calculation of free energies from non-equilibrium switching simulations relies on the ideas of waste-recycling Monte Carlo [37].

## 5. Calculating Potentials of Mean Force

Potentials of mean force (PMF) G(q) along a chosen coordinate q = q(x) are defined as:

In many practical applications, the biasing potentials V are harmonic. In such “steered molecular dynamics” simulations and similar approaches [42–45], one can obtain estimates of the PMF using approximate formalisms that involve the system’s free energy difference ΔF(t) and its time dependence. In the limit of very stiff pulling springs V (q, t) = k[q − z(t)]^{2}/2, constraining q to a prescribed path z(t) with large k, one can use the “stiff-spring approximation” of Park et al. [46]. In this limit, q is almost a control parameter, which results in an approximate relation between the system free energy difference ΔF (t) and the PMF G(q):

## 6. Importance Sampling of Fast-Switching Trajectories

Fast switching simulations carried out at large switching rates typically generate work distributions that lead to large statistical uncertainties in the free energy estimate. As discussed earlier, the reason is that trajectories with typical work values contribute little to the exponential average of the Jarzynski equation, while trajectories with work values dominating the average are very rare. As a consequence, the convergence of the computed free energy is impractically slow for overly fast switching. A solution to this problem consists in favoring the generation of trajectories with important work values. In this section, we discuss how path sampling techniques can be used for this purpose.

To introduce computational methods for realizing this idea, we rewrite the exponential work average as an explicit sum over pathways:

_{0}. As suggested by Ytreberg and Zuckerman [49] and by Athènes [50], one way to enhance the sampling of important trajectories consists in introducing an explicit bias function π[x(t) ] (assumed to be integrable and positive everywhere) in the average:

_{π}denote an average over pathways distributed according to the biased ensemble P

_{π}[x(t)] ∝ P [x(t)]π[x(t) ]. Since, in general, the bias function π[x(t) ] depends on the entire pathway x(t), the biased ensemble cannot be sampled by preparing initial conditions according to a certain distribution and running fast switching trajectories from them. Instead, one can use trajectory sampling algorithms (such as the shooting algorithm) adapted from transition path sampling, a methodology originally developed for the simulation of rare events occurring in complex systems [51–53]. In this approach, the bias function appears in the acceptance probability of the path sampling scheme, steering the simulation towards the desired regions of trajectory space.

Since the bias function should enhance the sampling of important, but rare, work values, a bias function depending on the path x(t) only through the work W [x(t) ] suffices, π[x(t) ] = π[W[x(t) ]]. The accuracy of a free energy calculation carried out with biased path sampling now crucially depends on the particular choice of this bias function. It is evident that to obtain an accurate estimate of ΔF, the bias function should be selected, such that the statistical error is small both in the numerator and in the denominator of the fraction on the right-hand side of Equation (25). This implies that the work distribution in the biased ensemble should have a large overlap with the work distribution P(W) in the unbiased ensemble, as well as with the integrand P(W) exp(−βW) appearing in the Jarzynski equality. It has been shown [49,50] that large efficiency increases can be obtained using the bias function π(W) = exp(−βW/2), which produces a work distribution in between the two distributions P(W) and P(W) exp(−βW) [54]. A more systematic investigation [55] of the statistical error in the free energy estimate obtained by biased path sampling yields the optimum bias π(W) = | exp(−β(W − ΔF)) − 1|. This result implies that the expected statistical error in the free energy is smallest if typical and dominant work values are sampled with high frequency. Interestingly, sampling work values W ≈ ΔF near the free energy difference is not important. Unfortunately, the practical usefulness of this optimum bias function is limited, because its application requires prior knowledge of the free energy difference, i.e., the very quantity one wants to compute. However, iterative schemes, in which the bias function is adapted as the simulation goes on, might make productive use of the functional form of the optimum bias. A recently suggested approach [36] based on the waste-recycling estimator [37] effectively introduces a bias that covers both peaks of the optimum bias, π(W).

Another way of realizing work biased path sampling of fast-switching trajectories for the computation of free energies was suggested by Sun [56,57]. In this approach, which can be viewed as a thermodynamic integration procedure in path space, a parameter α is introduced into the exponential average:

_{α}indicates a path average over the work weighted path ensemble:

_{α}is not affected by the type of statistical errors that make the computation of the exponential work average difficult, and it can be evaluated efficiently in a path sampling simulation. By repeating such a calculation for different values of α and integrating the work average numerically, one finally obtains the desired free energy difference. Furthermore, in this method, the statistical errors are kept low by making sure that pathways with both dominant and typical work values are sampled with sufficient frequency. This can be seen explicitly by noting that in the work biased ensemble corresponding to a particular value of the bias parameter, α, the work, W, is distributed according to P

_{α}(W) ∝ P (W) exp(−βαW). Thus, by gradually changing α from zero to one, one switches the work distribution from P(W) to P(W) exp(−βW), sweeping over all important work values in the course of the thermodynamic integration procedure.

One can show that in the limit of infinitely short trajectories, Sun’s method reduces to conventional thermodynamic integration. This result raises the question of which trajectory length leads to the most efficient free energy calculations and, in particular, if work biased path sampling algorithms perform better then conventional methods, such as thermodynamic integration or umbrella sampling. Extensive calculations carried out for various models indicate [58,59] that work biased fast switching path algorithms are generally less efficient than standard methods, such as thermodynamic integration, thermodynamic perturbation or umbrella sampling. There are however cases, such as an ideal gas compressed by a piston moving in a cylinder, where fast switching is advantageous [59]. In this particular case, the work distribution does not converge to a limiting form for increasing switching speed, and the typical work values keep growing. As a consequence, the optimum switching rate is finite in this case, even if an optimum work bias is applied [59].

## 7. Fast Switching with Large Time Steps

Molecular dynamics simulations are usually carried out with time steps that are a compromise between accuracy (often assessed in terms of energy conservation) and computing speed. Small time steps yield accurate trajectories with good energy conservation, but require a larger computational effort, because the cost of a trajectory of a given length is proportional to the number of steps and, hence, inversely proportional to the size of the time step. Larger time steps reduce the computing time, but corrupt the accuracy, resulting in poor energy conservation. In general, using such low-accuracy trajectories for free energy computations introduces a systematic error into the free energy estimate. It is, however, possible to devise exact expressions akin to the Jarzynski equation to compute free energy differences from crude trajectories calculated with large time steps [13,60]. Using this approach, which is based on a generalization of the Jarzynski equation for phase space mappings [61], can help to considerably increase the efficiency of fast switching simulations, due to the reduced computational cost of the large time step trajectories.

As mentioned earlier, in the limit of instantaneous switching, the Jarzynski equation reduces to the perturbation identity of Equation (6). Free energy computation methods relying on this equation perform well if there is a large overlap between the ensembles A and B, corresponding to the control parameters λ_{A} and λ_{B}, respectively. If, however, these ensembles strongly differ, the free energy calculation converges poorly, because important contributions to the average are rarely sampled. To remedy this situation, Jarzynski has devised the targeted free energy perturbation method [61] based on a generalization of the Jarzynski equality. The basic ideas underlying this approach is to improve the efficiency of the perturbative calculation by applying a mapping that transforms the equilibrium ensemble A into an ensemble A′ that overlaps more strongly with ensemble B. The mapping ϕ(x) considered in this approach is required to be invertible and differentiable, but is arbitrary otherwise. By starting from the definition of the free energy difference (Equation (3)) and carrying out a variable transformation from x to x′ = ϕ(x), one can then show that:

^{−1}, with the corresponding work definition, one can also use this mapping approach together with the Crooks fluctuation theorem.

Equation (30) suggests the following algorithm for free energy computation. One first samples phase space points x from the equilibrium ensemble A. Then, to each of these points, one applies the mapping and computes W_{ϕ}. Finally, the average of exp(−βW_{ϕ}(x)) carried out over all points x yields the free energy difference. Now, the efficiency of this method crucially depends on the ability to devise appropriate mapping ϕ(x). The closer the ensemble resulting from the transformation resembles B, the higher is the efficiency. No general methods exists to derive ϕ (x), but a well-chosen mapping can substantially reduce the cost of a free energy computation.

One possible strategy to exploit Equation (30) consists in choosing a sequence of molecular dynamics steps as phase space mapping. Each of these steps, designed to approximate the time evolution of the system over a small interval Δt maps a phase point x_{i} into the next phase point x_{i}_{+1} along the molecular dynamics trajectory. Hence, a sequence of n molecular dynamics steps may also be considered as a phase space mapping that takes the initial point x_{0} into the final point x_{n}. The expression for the work W_{ϕ} is particularly simple for integrators, such as the Verlet algorithm, that conserve phase space volume. Then, the Jacobian of the mapping is unity, and Equation (30) turns into:

The large time step formalism can also be used for the calculation of potentials of mean force [62]. In such a simulation, the work based reweighing of Equation (30) is applied at each stage of the time evolution with a work function that accumulates along the trajectory. Fast switching simulations were carried out for the force induced unfolding of a decalanine molecule [62]. The free energy profile obtained for a time step of 3.2 fs, i.e., close to the stability limit, agrees well with that calculated using a conservative time step of 0.5 fs. An efficiency analysis reveals that the optimum time step for the unfolding simulations lies in the range 1–3 fs. It is interesting to note that the fast-switching trajectories may show unphysical features, such as a redistribution from potential to kinetic energy, due to the conserved shadow Hamiltonian belonging to the integrator used in the simulation [62]. Nevertheless, the obtained free energy profile is exact up to statistical errors.

## 8. Applications

Arguably the most important practical application of non-equilibrium work theorems has been to experiments. Almost immediately after the connection between non-equilibrium single-molecule pulling experiments and Jarzynski’s identity was rigorously established [7], experimental studies of the folding and unfolding of nucleic acids using optical tweezers followed [8,63]. It is often difficult, if not impossible, to conduct pulling experiments sufficiently slowly to maintain near-equilibrium conditions. Nonetheless, the use of non-equilibrium free energy reconstruction has made it possible to extract thermodynamic information.

Applications to pulling have been mirrored on the simulation side. Simulated pulling methods mimicking experiments have been developed, initially to probe mechanical perturbations on biomolecules [42–44]. Non-equilibrium pulling methods have been applied not only to protein unfolding, but also to many other complex molecular processes, including ligand dissociation [64–66] and channel translocation [67,68]. To analyze such “steered molecular dynamics” simulations and extract PMFs, the stiff-spring approximation is widely used [46], though Equation (22) offers a more accurate method using the same information [47] that produce results comparable to full histogram reweighting. In molecular simulations, non-equilibrium methods tend to be less efficient than optimized equilibrium methods as a tool to calculate free energies [18,58]. However, as discussed above, the optimization of non-equilibrium sampling methods is an area of active research, in particular, using importance sampling methods involving path reweighting [49,50,55–59] and nonlinear maps [69,70]. Moreover, non-equilibrium methods can provide valuable insight into the mechanism underlying a process. By forcing the system through a transition and monitoring the resulting bottlenecks [71], one may be able to devise improved control variables that result in a smoother transition and improved sampling efficiency, both in non-equilibrium and equilibrium simulations.

## 9. Conclusions and Outlook

The Jarzynski non-equilibrium work theorem and the Crooks fluctuation theorem are fundamental exact relations that link the irreversible work carried out on a system during a non-equilibrium transformation to the system’s equilibrium statistics. To date, the most significant application of these relations lies in the interpretation of single-molecule pulling experiments, in which forces exerted by atomic force microscopes or optical tweezers are used to probe the properties of individual molecules. Due to technological limitations, such experiments are necessarily carried out at a finite pulling rate, leading to non-equilibrium effects that cannot be neglected. The theorems of Jarzynski and Crooks provide a practical tool for the interpretation of such single-molecule pulling experiments and permit one to extract equilibrium information, such as potentials of mean force, from data obtained under inherently non-equilibrium conditions [7–9,72].

From a computational point of view, the Jarzynski and Crooks theorems have provided a new and powerful framework for the calculation of free energies using computer simulations. Apart from putting earlier slow-growth free energy simulations on a firm theoretical footing, these results have spawned the development of several new free energy algorithms based on non-equilibrium, fast-switching trajectories.

Depending on the rate at which the system is driven away from equilibrium, fast switching free energy computations can be plagued by large statistical errors. For strong driving, i.e., for large switching rates, work distributions are broad, with typical work values by far exceeding the free energy difference. As a consequence, the exponential work average of the Jarzynski equation is dominated by a few rare contributions, leading to large statistical uncertainties and a bias in the free energy estimate. Such errors can outweigh the computational advantage of running inexpensive short trajectories rather than one single long trajectory [18,29,58]. In fact, it has been shown that in the slow switching regime, one obtains more accurate results from few slow simulations than from many faster ones [18]. Numerical simulations carried out for various model systems [58,59] indicate that conventional free energy computation methods, such as thermodynamic integration or free energy perturbation theory, are more efficient than fast switching simulations, even if work biasing techniques are employed. Fast switching methods may, however, be advantageous for systems in which the states of interest are connected by several distinct pathways. In such a case, conventional methods may fail to sample all important transition routes while multiple fast switching trajectories have the chance to probe all important pathways. Such a situation was indeed observed for transitions between low-energy configurations of Lennard-Jones clusters [41], which could be sampled successfully only with non-equilibrium path sampling, but not with other approaches. Compared to standard methods, fast switching algorithms appeared on the scene only recently, such that substantial improvements and new developments are to be expected [13,21,57,73–78]. It is worth noting that fast switching ideas have not only been applied to the calculation of free energies, but have also been combined with existing sampling methods to enhance the efficiency of the simulation. For instance, non-equilibrium switches have been used to improve the acceptance probability of replica exchange simulations [79,80] and to generate trial configurations for Monte Carlo simulations [81,82]. Conversely, waste-recycling Monte Carlo [37] can be adapted for the calculation of free energies from non-equilibrium switching simulations [36].

One aspect of fast switching simulations that has not been fully exploited is the freedom in choosing the transformation protocol. While the optimization of the time dependence of the driving parameter has been the subject of previous numerical and analytical studies [23,24], the extension of such optimizations to multiple control parameters is unexplored to date. The control parameter at the start and the end of the transformation are given, but in between, additional parameters can be subjected to a change as well, without affecting the validity of the relations that provide the basis for fast switching simulation. As an early example, an external pressure has been heuristically adjusted to maintain reasonable box sizes and prevent phase separation in a transformation between liquid and ideal gas states [54]. Defining parameter spaces of higher dimension and determining optimum parameter pathways in these spaces may offer efficient ways to control the work distribution and, hence, reduce the computational cost of fast switching simulations.

We acknowledge the financial support of the Austrian Science Fund (FWF, Fonds zur Förderung der Wissenschaftlichen Forschung) within the SFB ViCoM (Spezialforschungsbereich Vienna Computational Materials Laboratory), Grant F41, as well as Project P24681-N20 (C.D.) and the Max Planck Society (G.H.).

## Conflicts of Interest

The authors declare no conflict of interest.

## References

- Frenkel, D. Free-Energy Computation and First-Order Phase Transitions. In Molecular Dynamics Simulations of Statistical Mechanical Systems, Proceedings of the Enrico Fermi Summer School, Varenna, 1985; Ciccotti, G., Hoover, W.G., Eds.; North-Holland Elsevier Science Publisher: Amsterdam, The Netherlands, 1987; pp. 151–188. [Google Scholar]
- Jarzynski, C. Nonequilibrium equality for free energy differences. Phys. Rev. Lett
**1997**, 78, 2690–2693. [Google Scholar] - Jarzynski, C. Equilibrium free energy differences from nonequilibrium measurements: A master-equation approach. Phys. Rev. E
**1997**, 56, 5018–5035. [Google Scholar] - Crooks, G.E. Path-ensemble averages in systems driven far from equilibrium. Phys. Rev. E
**2000**, 61, 2361–2366. [Google Scholar] - Crooks, G.E. Nonequilibrium measurements of free energy differences for microscopically reversible Markovian systems. J. Stat. Phys
**1998**, 90, 1481–1487. [Google Scholar] - Crooks, G.E. Entropy production fluctuation theorem and the nonequilibrium work relation for free energy differences. Phys. Rev. E
**1999**, 60, 2721–2726. [Google Scholar] - Hummer, G.; Szabo, A. Free energy reconstruction from nonequilibrium single-molecule pulling experiments. Proc. Natl. Acad. Sci. USA
**2001**, 98, 3658–3661. [Google Scholar] - Liphardt, J.; Dumont, S.; Smith, S.B.; Tinoco, I.; Bustamante, C. Equilibrium information from nonequilibrium measurements in an experimental test of Jarzynski’s equality. Science
**2002**, 296, 1832–1835. [Google Scholar] - Noy, A. Direct determination of the equilibrium unbinding potential profile for a short DNA duplex from force spectroscopy data. Appl. Phys. Lett
**2004**, 85, 4792–4794. [Google Scholar] - Chipot, C.; Pohorille, A. Free Energy Calculations; Spinger Series in Chemical Physics 86. Springer: Berlin/Heidelberg, Germany, 2007. [Google Scholar]
- Lelièvre, T.; Rousset, M.; Stoltz, G. Free Energy Computations; Imperial College Press: London, UK, 2010. [Google Scholar]
- Frenkel, D.; Smit, B. Understanding Molecular Simulation: From Algorithms to Applications; Academic Press: San Diego, CA, USA, 2001. [Google Scholar]
- Lechner, W.; Oberhofer, H.; Dellago, C.; Geissler, P.L. Equilibrium free energies from fast-switching trajectories with large time steps. J. Chem. Phys
**2006**, 124, 044113. [Google Scholar] - Schöll-Paschinger, E.; Dellago, C. A proof of Jarzynski’s nonequilibrium work theorem for dynamical systems that conserve the canonical distribution. J. Chem. Phys
**2006**, 125, 054105. [Google Scholar] - Zwanzig, R.W. High-temperature equation of state by a perturbation method. I. Nonpolar gases. J. Chem. Phys
**1954**, 22, 1420–1426. [Google Scholar] - Kirkwood, J. Statistical mechanics of fluid mixtures. J. Chem. Phys
**1935**, 3, 300. [Google Scholar] - Hummer, G.; Szabo, A. Free energy surfaces from single-molecule force spectroscopy. Acc. Chem. Res
**2005**, 38, 504–513. [Google Scholar] - Hummer, G. Fast-growth thermodynamic integration: Error and efficiency analysis. J. Chem. Phys
**2001**, 114, 7330–7337. [Google Scholar] - Jarzynski, C. Rare events and the convergence of exponentially averaged work values. Phys. Rev. E
**2006**, 73, 046105. [Google Scholar] - Schmiedl, T.; Seifert, U. Optimal finite-time processes in stochastic thermodynamics. Phys. Rev. Lett
**2007**, 98, 108301. [Google Scholar] - De Koning, M. Optimizing the driving function for nonequilibrium free-energy calculations in the linear regime: A variational approach. J. Chem. Phys
**2005**, 122, 104106. [Google Scholar] - Then, H.; Engel, A. Computing the optimal protocol for finite-time processes in stochastic thermodynamics. Phys. Rev. E
**2008**, 77, 041105. [Google Scholar] - Gomez-Marin, A.; Schmiedl, T.; Seifert, U. Optimal protocols for minimal work processes in underdamped stochastic thermodynamics. J. Chem. Phys
**2008**, 129, 024114. [Google Scholar] - Geiger, P.; Dellago, C. Optimum protocol for fast switching free energy calculations. Phys. Rev. E
**2010**, 81, 021127. [Google Scholar] - Wood, R.H.; Mühlbauer, W.C.F.; Thompson, P.T. Systematic errors in free energy perturbation calculations due to a finite sample of configuration space. Sample-size hysteresis. J. Phys. Chem
**1991**, 95, 6670–6675. [Google Scholar] - Gore, J.; Ritort, F.; Bustamante, C. Bias and error in estimates of equilibrium free-energy differences from nonequilibrium measurements. Proc. Natl. Acad. Sci. USA
**2003**, 100, 12564–12569. [Google Scholar] - Zuckerman, D.M.; Woolf, T.B. Theory of a systematic computational error in free energy differences. Phys. Rev. Lett
**2002**, 89, 180602. [Google Scholar] - Wu, D.; Kofke, D.A. Asymmetric bias in free-energy perturbation measurements using two hamiltonian-based models. Phys. Rev. E
**2004**, 70, 066702. [Google Scholar] - Rodriguez-Gomez, D.; Darve, E.; Pohorille, A. Assessing the efficiency of free energy calculation methods. J. Chem. Phys
**2004**, 120, 3563–3578. [Google Scholar] - Ozer, G.; Quirk, S.; Hernandez, R. Thermodynamics of decaalanine stretching in water obtained by adaptive steered molecular dynamics simulations. J. Chem. Theory Comput
**2012**, 8, 4837–4844. [Google Scholar] - Zuckerman, D.M.; Woolf, T.B. Overcoming finite-sampling errors in fast-switching free-energy estimates. Extrapolative analysis of a molecular system. Chem. Phys. Lett
**2002**, 351, 445–453. [Google Scholar] - Ytreberg, F.M.; Zuckerman, D.M. Efficient use of nonequilibrium measurement to estimate free energy differences for molecular systems. J. Comp. Chem
**2004**, 25, 1749–1759. [Google Scholar] - Bennett, C.H. Efficient estimation of free energy differences from Monte Carlo data. J. Comput. Phys
**1976**, 22, 245–268. [Google Scholar] - Shirts, M.R.; Bair, E.; Hooker, G.; Pande, V.S. Equilibrium free energies from nonequilibrium measurements using maximum-likelihood methods. Phys. Rev. Lett
**2003**, 91, 140601. [Google Scholar] - Hummer, G.; Szabo, A. Calculation of free energy differences from computer simulations of initial and final states. J. Chem. Phys
**1996**, 105, 2004–2010. [Google Scholar] - Adjanor, G.; Athènes, M. J.; Rodgers, J. Waste-recycling Monte Carlo with optimal estimates: Application to free energy calculations in alloys. J. Chem. Phys
**2011**, 135, 044127. [Google Scholar] - Frenkel, D. Speed-up of Monte Carlo simulations by sampling of rejected states. Proc. Natl. Acad. Sci. USA
**2004**, 101, 17571–17575. [Google Scholar] - Ferrenberg, A.M.; Swendsen, R.H. Optimized Monte Carlo data analysis. Phys. Rev. Lett
**1989**, 63, 1195–1198. [Google Scholar] - Oberhofer, H.; Dellago, C. Efficient extraction of free energy profiles from non-equilibrium experiments. J. Comput. Chem
**2009**, 30, 1726–1736. [Google Scholar] - Imparato, A.; Peliti, L. Evaluation of free energy landscapes from manipulation experiments. J. Stat. Mech
**2006**, 2006, P03005. [Google Scholar] - Athènes, M.; Marinica, M.-C. Free energy reconstruction from steered dynamics without post-processing. J. Comput. Phys
**2010**, 229, 7129–7146. [Google Scholar] - Grubmüller, H.; Heymann, B.; Tavan, P. Ligand binding molecular mechanics calculation of the streptavidin biotin rupture force. Science
**1996**, 271, 997–999. [Google Scholar] - Izrailev, S.; Stepaniants, S.; Balsera, M.; Oono, Y.; Schulten, K. Molecular dynamics study of unbinding of the avidin-biotin complex. Biophys. J
**1997**, 72, 1568–1581. [Google Scholar] - Paci, E.; Karplus, M. Forced unfolding of fibronectin Type 3 modules: An analysis by biased molecular dynamics simulations. J. Mol. Biol
**1999**, 288, 441–459. [Google Scholar] - Park, S.; Schulten, K. Calculating potentials of mean force from steered molecular dynamics simulations. J. Chem. Phys
**2004**, 120, 5946–5961. [Google Scholar] - Park, S.; Khalili-Araghi, F.; Tajkhorshid, E.; Schulten, K. Free energy calculation from steered molecular dynamics simulations using Jarzynski’s equality. J. Chem. Phys
**2003**, 119, 3559–3566. [Google Scholar] - Hummer, G.; Szabo, A. Free energy profiles from single-molecule pulling experiments. Proc. Natl. Acad. Sci. USA
**2010**, 107, 21441–21446. [Google Scholar] - Minh, D.D.L.; Adib, A.B. Optimized free energies from bidirectional single-molecule force spectroscopy. Phys. Rev. Lett
**2008**, 100, 180602. [Google Scholar] - Ytreberg, F.M.; Zuckerman, D.M. Single-ensemble nonequilibrium path-sampling estimates of free energy differences. J. Chem. Phys
**2004**, 120, 10876–10879. [Google Scholar] - Athènes, M. A path-sampling scheme for computing thermodynamic properties of a many-body system in a generalized ensemble. Eur. Phys. J. B
**2004**, 38, 651. [Google Scholar] - Dellago, C.; Bolhuis, P.G.; Csajka, F.S.; Chandler, D. Transition path sampling and the calculation of rate constants. J. Chem. Phys
**1998**, 108, 1964. [Google Scholar] - Dellago, C.; Bolhuis, P.G.; Geissler, P.L. Transition path sampling. Adv. Chem. Phys
**2002**, 123, 1–84. [Google Scholar] - Dellago, C.; Bolhuis, P.G.; Geissler, P.L. Transition Path Sampling Methods. In Computer Simulations in Condensed Matter: From Materials to Chemical Biology; Ciccotti, G., Binder, K., Eds.; Springer: Berlin/Heidelberg, Germany, 2006. [Google Scholar]
- Adjanor, G.; Athènes, M. Gibbs free-energy estimates from direct path-sampling computations. J. Chem. Phys
**2005**, 123, 234104. [Google Scholar] - Oberhofer, H.; Dellago, C. Optimum bias for fast-switching free energy calculations. Comput. Phys. Commun
**2008**, 179, 41–45. [Google Scholar] - Sun, S.X. Equilibrium free energies from path sampling of nonequilibrium trajectories. J. Chem. Phys
**2003**, 118, 5769–5775. [Google Scholar] - Atilgan, E.; Sun, S.X. Equilibrium free energy estimates based on nonequilibrium work relations and extended dynamics. J. Chem. Phys
**2004**, 121, 10392–10400. [Google Scholar] - Oberhofer, H.; Dellago, C.; Geissler, P.L. Biased sampling of nonequilibrium trajectories: Can fast switching simulations outperform conventional free energy calculation methods? J. Phys. Chem. B
**2005**, 109, 6902–6915. [Google Scholar] - Lechner, W.; Dellago, C. On the efficiency of path sampling methods for the calculation of free energies from non-equilibrium simulations. J. Stat. Mech
**2007**, 2007, P04001. [Google Scholar] - Oberhofer, H.; Dellago, C. Large timestep fast-switching simulations with non-volume preserving integrators for free energy calculations. Isr. J. Chem
**2007**, 47, 215. [Google Scholar] - Jarzynski, C. Targeted free energy perturbation. Phys. Rev. E
**2002**, 65, 046122. [Google Scholar] - Oberhofer, H.; Dellago, C.; Boresch, S. Single molecule pulling with large time steps. Phys. Rev. E
**2007**, 75, 061106. [Google Scholar] - Collin, D.; Ritort, F.; Jarzynski, C.; Smith, S.B.; Tinoco, I.; Bustamante, C. Verification of the Crooks fluctuation theorem and recovery of RNA folding free energies. Nature
**2005**, 437, 231–234. [Google Scholar] - Vashisth, H.; Abrams, C. F. Ligand escape pathways and (un)binding free energy calculations for the hexameric insulin-phenol complex. Biophys. J
**2008**, 95, 4193–4204. [Google Scholar] - Cuendet, M.A.; Michielin, O. Protein-protein interaction investigated by steered molecular dynamics the Tcr-Pmhc complex. Biophys. J
**2008**, 95, 3575–3590. [Google Scholar] - Zhang, D.Q.; Gullingsrud, J.; McCammon, J.A. Potentials of mean force for acetylcholine unbinding from the alpha7 nicotinic acetylcholine receptor ligand-binding domain. J. Am. Chem. Soc
**2006**, 128, 3019–3026. [Google Scholar] - Jensen, M.O.; Park, S.; Tajkhorshid, E.; Schulten, K. Energetics of glycerol conduction through aquaglyceroporin Glpf. Proc. Natl. Acad. Sci. USA
**2002**, 99, 6731–6736. [Google Scholar] - Amaro, R.; Luthey-Schulten, Z. Molecular dynamics simulations of substrate channeling through an alpha-beta barrel protein. Chem. Phys
**2004**, 307, 147–155. [Google Scholar] - Vaikuntanathan, S.; Jarzynski, C. Escorted free energy simulations: Improving convergence by reducing dissipation. Phys. Rev. Lett
**2008**, 100, 190601. [Google Scholar] - Vaikuntanathan, S.; Jarzynski, C. Escorted free energy simulations. J. Chem. Phys
**2011**, 134, 054107. [Google Scholar] - Chelli, R. Local sampling in steered monte carlo simulations decreases dissipation and enhances free energy estimates via nonequilibrium work theorems. J. Chem. Theory Comput
**2012**, 8, 4040. [Google Scholar] - Trepagnier, E.H.; Jarzynski, C.; Ritort, F.; Crooks, G.E.; Bustamante, C.J.; Liphardt, J. Experimental test of Hatano and Sasa’s nonequilibrium steady-state equality. Proc. Natl. Acad. Sci. USA
**2004**, 101, 15038–15041. [Google Scholar] - Shirts, M.R.; Pande, V.S. Comparison of efficiency and bias of free energies computed by exponential averaging, the Bennett acceptance ratio and thermodynamic integration. J. Chem. Phys
**2005**, 122, 144107. [Google Scholar] - Ytreberg, F.M.; Zuckerman, D.M. Peptide conformational equilibria computed via a single-stage shifting protocol. J. Phys. Chem. B
**2005**, 109, 9096–9103. [Google Scholar] - Chernyak, V.; Chertkov, M.; Jarzynski, C. Dynamical generalization of nonequilibrium work relation. Phys. Rev. E
**2005**, 71, 025102. [Google Scholar] - Rodinger, T.; Pomès, R. Enhancing the accuracy the efficiency and the scope of free energy simulations. Curr. Opin. Struct. Biol
**2005**, 15, 164–170. [Google Scholar] - Lua, R.C.; Grosberg, A.Y. Practical applicability of the Jarzynski relation in statistical mechanics: A pedagogical example. J. Phys. Chem. B
**2005**, 109, 6805–6811. [Google Scholar] - Adib, A.B. Entropy and density of states from isoenergetic nonequilibrium processes. Phys. Rev. E
**2005**, 71, 056128. [Google Scholar] - Ballard, A.J.; Jarzynski, C. Replica exchange with nonequilibrium switches. Proc. Natl. Acad. Sci. USA
**2009**, 106, 12224–12229. [Google Scholar] - Ballard, A.J.; Jarzynski, C. Replica exchange with nonequilibrium switches: Enhancing equilibrium sampling by increasing replica overlap. J. Chem. Phys
**2012**, 136, 194101. [Google Scholar] - Athènes, M. Computation of a chemical potential using a residence weight algorithm. Phys. Rev. E
**2002**, 66, 046705. [Google Scholar] - Nilmeier, J.P.; Crooks, G.E.; Minh, D.L.; Chodera, J.D. Nonequilibrium candidate Monte Carlo is an efficient tool for equilibrium simulation. Proc. Natl. Acad. Sci. USA
**2011**, 108, E1009. [Google Scholar]

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