*2.7. Statistical Analysis*

Data is represented as mean ± SD for group characteristics and mean ± SEM for comparison of group means. The differences between paired and unpaired data were determined by two-tailed Students's *t-*tests and *P* values ≤ 0.05 were considered significant. Analysis of variance with Fisher pairwise multiple comparison procedure was carried out using *SigmaStat* software [22].
