2.6.4. Treatment of Nucleoids with Vitamin C

In order to check the direct effect of vitamin C on DNA, non-damaged HeLa cell nucleoids, or substrate nucleoids prepared as described in Section 2.6.1, were treated for 10 or 20 min with vitamin C dissolved in buffer A at 0, 1, 5 or 25 μM and diluted with buffer F, as described in Section 2.6.2. The reaction was carried out as described in Section 2.6.3. The final concentrations of vitamin C were therefore 0, 0.25, 1.25, and 6.25 μM.
