*2.9. Statistical Analysis*

Statistical analysis was performed using SAS 9.3 and GraphPad Prism 6.0 (GraphPad Software, San Diego, CA, USA). Data are expressed as mean ± SE. Results were compared using Student-Newman-Keuls test or one-way ANOVA and the *post hoc* Tukey test to identify specific differences between groups. Statistical significance was confirmed at a *p* value of <0.05.

**Figure 1.** Vitamin C sufficient Gulo−/− mice demonstrate reduced lung NETs and lower cf-DNA following peritonitis-induced sepsis. Representative immunofluorescence and differential interference contrast imaging of lung NETs (**A**–**E**): (**A**) VitC sufficient Gulo−/− mice (**+**) received saline alone (0.4 mL, i.p.); (**B**) FIP exposed VitC sufficient Gulo−/− mice [**FIP**(**+**)] received fecal stem solution (45 mg/mL, i.p.) followed 30 min later by saline (0.1 mL, i.p.); (**C**) VitC deficient Gulo−/− mice (**−**) received saline alone (0.4 mL, i.p.); (**D**) FIP exposed VitC deficient Gulo−/− mice [**FIP**(**−**)] mice received fecal stem solution (45 mg/mL, i.p.) followed 30 min later by saline (0.1 mL, i.p.). (**E**) AscA treated FIP exposed VitC deficient Gulo−/− mice [**FIP**(**−**) **+ AscA**] mice received fecal stem solution (45 mg/mL, i.p.) followed 30 min later by AscA (200 mg/kg, i.p.). Platelet CD-41 (green), histones (red), and myeloperoxidase (grey) are seen in the merged images. Arrowheads indicate NET formation shown by co-staining for platelet CD-41 (green), histones (red), myeloperoxidase (grey), and DAPI (blue) in the vascular and alveolar spaces. Arrows indicate extensive extra-nuclear histones (red); (10× magnification, *N* = 3 for each group). (**F**) Serum levels of cf-DNA were quantified using the Quant-iTPicoGreen dsDNA assay kit (*N* = 5–11 for each group, *p* < 0.05).
