2.6.3. The Reaction

The substrate cells were thawed, diluted in cold PBS, centrifuged at 800× *g* for 5 min at 4 °C and resuspended in PBS at a concentration of 1 × 106 cells per mL. As described in section 2.4 gels were made, put in lysis for 1 h and washed 3 times (5 min each time) with buffer F. Then 30 μL of extract (experimental treatment), FPG (positive control), or buffer F (negative control) were placed on each gel (two gels per condition) and covered with a cover slip. Slides were placed in a moist box and incubated for 10 and 20 min. Afterwards the rest of the comet assay was performed as described above.
