*3.4. Statistics*

Statistical analysis was performed by GraphPad Prism software, version 6 (GraphPad Software, Inc., La Jolla, CA, USA). Statistical differences between ascorbate samples were determined by two tailed ANOVA using a Tukey post-hoc analysis, with a *p*-value below 0.05 considered significant. Non-linear regression analysis was used to determine the half-life of ascorbate in PBS or media.
