Drylands cover 40% of the global surface and house more than 2 billion people. Drought-tolerant crops are becoming more important in these regions, not only to provide food, fodder, and energy, but also to sequester soil organic carbon. This study evaluated soil and root system attributes of forage cactus ‘Orelha de Elefante Mexicana’ (Opuntia stricta Haw.) managed using different agronomic practices in the Brazilian Semiarid. The experiment was established in June 2011 and the design was split-plot in a randomized complete blocks, where the main plot was the different planting density, and the subplots were the factorial arrangement between harvest frequency and harvest intensity. Soil samples were collected at 0 to 10 and 10 to 20 cm depths and response variables included root biomass, soil bulk density (BD), and soil carbon (C) and nitrogen (N) contents and stocks. Sampling occurred in August 2019, but for root biomass and soil BD analysis it also occurred in September 2021. There were no significant effects from management practices on root biomass at 0 to 10 and 10 to 20 cm depth (p > 0.05), with respective averages of 12.45 Mg ha⁻¹ and 6.06 Mg ha⁻¹. Soil BD was similar at 10 to 20 cm depth (p > 0.05) averaging 1.28 g cm⁻³. Soil organic carbon (SOC) stock varied with management and reached almost 100 Mg C ha⁻¹ in the 0 to 20 cm layer, indicating the potential of cactus to store carbon. Plants with a more developed root system are more likely to survive the drought climatic condition; therefore, less dense plantings could result in more resilient plants for drier regions, but could potentially negatively affect biomass productivity per area. Full article

Accurate inversion of soil hydraulic parameters based on the van Genuchten–Mualem model has received much attention in soil science research. Herein, a hybrid algorithm method using particle swarm optimization and vector-evaluated genetic algorithm was used to invert the parameters θ_s, α, n, and K_s, with the objective functions of infiltration rate, cumulative infiltration, and soil water content. Then, numerical experiments were conducted on four typical soils at three initial water content levels (20, 40, and 60% effective saturation) to verify the accuracy of the inverse method. The results showed that the inversed soil water retention and conductivity curves were approximately the same as the real curves, with the root mean square errors of 0.00101–0.00192 cm³·cm⁻³, 0.00800–0.02519 cm³·cm⁻³, respectively, and both the Nash-Sutcliffe coefficients were approximately 1.0. Additionally, laboratory experiments were also performed to compare with the inversed parameters for verification, within small root mean squared errors and approximately 1.0 Nash–Sutcliffe coefficients. Furthermore, the method can also achieve acceptably accurate parameter inversion even with substantial measurement errors included in the cumulative infiltration, initial water content, and final water content. Thus, the method is effective and robust and found to be practical in field experiments. Full article

Olive mill solid waste (OMSW) is an agro-industrial waste that has a high content of recalcitrant lignocellulose, which can adversely affect the environment. This study aimed to evaluate the phenol and lignin removal and the enzyme activity involved in the biological pre-treatment of OMSW supplemented with aquaculture sludge (AS) as an external nitrogen source by Anthracophyllum discolor. The highest lignin removal and enzymatic activity performance was obtained in the mixture of OMSW and AS prepared at a C/N ratio 45. In these conditions, the pre-treatment could remove 66% of lignin and 68% of phenols in the solid phase and 56% of phenols in the liquid phase and the maximum activity of laccase, manganese peroxidase and manganese independent peroxidase were of 10, 289 and 75 U L⁻¹ in 25, 30, and 15 days of pre-treatment, respectively. These results propose that the addition of AS as a co-substrate for adjusting the C/N ratio allows a 41 and 141% increase in lignin removal and manganese peroxidase activity respectively, enabling the treatment of both OMSW and AS wastes and the possible recovery of an enzymatic extract of biotechnological interest. Full article

One of the crucial issues in developing nations is diminishing the yield gaps. Therefore, accurate yield gap estimation has many real-world uses for increasing crop production. Utilizing comparative performance analysis (CPA) techniques, the yield gap of wheat fields was evaluated in this study. In Varamin, Tehran Province, Iran, data on 104 wheat fields were collected between 2018 and 2020 and every aspect of wheat field management has been documented. The CPA model determines the yield gap’s contributing factors and potential yield. The results of data analysis revealed that the production ranged from 2600 to 7600 kg ha⁻¹. The CPA method predicted a potential yield of 9316 kg ha⁻¹ and found a yield gap of 3748 kg ha⁻¹; this amount was 40.23% of the potential yield. Leaf chlorophyll (29%), irrigation at stem extension (9%), LAI (7.7%), soil salinity (8.2%), field area (16.3%), phosphorus consumption (6%), nitrogen utilized at the stage of tillering (16%), and HI (7.8%) all contributed to the yield gap in the CPA. It has been said that the computed yield in CPA is a potential yield that can be reached. CPA is a cheap and straightforward tool that could identify yield gaps and their causes in a district without the need for costly experiments. Therefore, developing nations with significant efficiency and yield gaps can use these techniques effectively. Full article

Soil compaction was largely studied in different scenarios with laboratory and field scale experiments, with various soil conditions and traffic intensities. However, a detailed analysis to better understand the protective role of plant residues or cover crop mulch is still required. A field test was conducted in Northeast Italy aiming to fill this gap. Rye was chosen as a winter cover crop, and growth on a controlled traffic random block experimental field. Four different cover crop mulch treatments were compared to study the effects of root systems: roller crimper, flail mower, bare soil control and harvested biomass control. Four different traffic intensities were used to evaluate the multiple passages with 0, 1, 3, 5 traffic events. During traffic events, the mean normal stress was measured. Penetration resistance was then evaluated after trafficking and soil samples were collected. The obtained results showed a 19.3% cone index increase in bare soil compared to flail mower treatment after the first traffic event, while low differences were found in harvested biomass bulk density during the first and third traffic events. Moreover, mean normal stress increased 16.5% on harvested biomass treatment compared to the flail mower. These findings highlight that the cover crop maintains a lower soil penetration resistance during compaction events, helping the subsequent field operations. Furthermore, roller crimper and flail mower cover crop termination impact soil bearing capacity differently due to different soil moisture content. However, the results showed a low contribution of cover crop mulch on mitigating soil compaction effects during the experiment. Full article

Glyphosate, the most applied herbicide globally, offers effective non-selective and post-emergent weed control. Evolution of herbicide-resistant weeds is commonly associated with the recurrent application of herbicides with the same mode of action. Native to South America, hairy fleabane (Erigeron bonariensis L.) is the most problematic weed in this sub-continent and has previously been confirmed glyphosate resistant. This research aimed at characterizing glyphosate-resistant populations, thus estimating the frequency of resistance, resistance levels and identifying effective herbicide alternatives to control glyphosate-resistant populations. Glyphosate resistance characterization was initially conducted on ten suspected populations collected in plantain, banana, cassava, passionfruit, papaya, and drybean crops. Two resistant populations were selected and further characterized through dose-response tests; in addition, response to alternative herbicides (paraquat, glufosinate, 2,4-D, pyraflufen-ethyl, and mesotrione) was determined. All surveyed hairy fleabane populations survived (≥80% of individuals) the recommended glyphosate rate of 1080 g ae ha⁻¹; conversely, five populations collected from non-cropping areas were effectively controlled at this same rate. Dose-response tests estimated resistance factors of 3.15- to 22.3-fold versus the most susceptible population. Interestingly, resistance profile and dose-response tests detected hormesis responses at field-recommended rates. The most effective herbicide alternatives to control glyphosate-resistant hairy fleabane were pyraflufen-ethyl and mesotrione. Full article

This study was carried out to determine the life span of Xanthomonas euvesicatoria (Xeu) in seeds, soil and plant residues and the first inoculum source that cause the onset of the disease. The study was carried out in the province of Tokat. Rifampicin-resistant Xeu isolate was inoculated into soil, seeds and plant residues. As a result of the studies, it was determined that Xeu remained viable in inoculated seeds until the 220th day (7 months), and lived in the soil for 105 days in summer and 14 days in winter. In plant residues, it was determined that the the life span in summer period was 60 days at 0 cm depth, 30 days at 20 cm depth; 30 days at 0 cm depth and 15 days at 20 cm depth in that of winter period. According to the results of this study, it was determined that the Xeu did not survive for a long time in plant residues and soil in Tokat province and thus was not carried to the next year, on the other hand the seed was the most important source of inoculum. Full article

Tomato is a major crop in Paraguay, where it provides a source of employment and income for households. Tomato production can be affected by root-knot nematodes, especially Meloidogyne spp. The unequivocal identification of Meloidogyne spp. in Paraguay has not been conducted yet. This study aims to identify Meloidogyne species in eight tomato production districts of this country by biochemical and molecular techniques. Females of Meloidogyne spp. were extracted from tomato roots and characterized using esterase isozyme phenotypes. In addition, DNA was extracted from nematode eggs, and species-specific SCARs (sequence-characterized amplified regions) were used to confirm the diagnosis. Nematodes were detected in 100% of studied samples (prevalence), of which M. incognita (Est: I2, Rm: 1.1;1.2) and M. javanica (Est: J3, Rm: 1.0, 1.20, 1.35) were present in 39.13% and 26.08% of samples, respectively. One population (8.69%) of Meloidogyne sp. presenting an atypical esterase profile (Rm: 1.0 and 1.3) was only detected in Julián Augusto Saldívar District. Mixed populations, mostly M. incognita and M. javanica, were observed in 26.08% of samples. The SCAR primers incK14F/incK14R amplified specific fragments for M. incognita (399 bp) and M. javanica (670 bp), confirming the enzymatic results. Here, we present the first study of root-knot nematode identification at the species level in Paraguay. Full article

Protected cultivation is a resource-efficient method of crop production, however, at the same time, it is resource intensive. An optimum rate and time of fertilizer application is required in order to maximize crop yield vis-à-vis resource use efficiency. However, these aspects are scarcely studied for tomato under low- and medium-tech greenhouses in Indian humid sub-tropics. In this regard, a two-year study was conducted to assess the effect of four NPK fertilization rates (i.e., 120, 100, 80, and 60% of the recommended dose of fertilizers, RDF) and three fertigation scheduling approaches—fertigation at different stages in different proportions of NPK, along with an additional treatment, i.e., farmers’ practice (soil-based application of recommended NPK) for tomatoes under a naturally ventilated polyhouse. The plant growth attributes, the tomato yield- and quality-related traits, the nutrient (NPK) accumulation by the plants, the water use efficiency (WUE), and economics were studied in response to different fertigation rates and scheduling approaches. These parameters were affected by both the rates of NPK fertilization and their time of application (scheduling) over the different growth stages. Among the different rates and time of fertigation, the recommended dose of fertilizer (100% RDF) (i.e., 300 kg N, 150 kg P₂O₅, and 150 kg K₂O per ha and their scheduling as 15% N, 10% P₂O₅, and 10% K₂O of RDF during 15–45 days after transplanting (P₁); 40% N, 40% P₂O₅, and 40% K₂O of RDF during 47–76 DAT (P₂); 30% N, 40% P₂O₅, and 40% K₂O of RDF during 77–107 DAT (P₃); and 15% N, 10% P₂O₅, and 10% K₂O of RDF during 108–138 DAT (P₄)) was found to be the optimum for fruit yield, WUE, and economics of tomato under protected condition. Full article

Ergot disease (Claviceps paspali) and sterility have the potential to reduce seed yield in bahiagrass (Paspalum notatum Flüggé). Our objective was to determine the genotypic and environmental effects on seed yield, and the levels of ergot and sterility. Seventeen genotypes were evaluated in three locations across two years for seed yield, ergot severity, and total seed fractions (percentage of full seeds, ergot sclerotia and empty seeds). The percentage of full seeds and total seed yield were correlated (r = 0.52) but both were negatively correlated with the percentage of ergot sclerotia (r = −0.81 and −0.51). Empty seeds were negatively correlated with full seeds (r = −0.39), but not with ergot sclerotia and total seed yield. The genotype effect was significant for total seed yield and severity of infection with large heritabilities (H² = 0.92 and 0.86) but had no effect on the percentage of the seed fractions. Location and the interaction with genotype explained most of the variation in the percentage of the seed fractions and their weight. Ergot and sterility were significant detrimental factors, genotype was a major determinant of ergot levels and seed yield, while non-genetic factors were co-determinants in constructing the seed yield potential but were the main determinants of the yield potential realization. Full article

Agricultural industries are the foundation of the modernization of agricultural and rural areas in China. National Modern Agricultural Industrial Parks (NMAIPs) provides a considerable nationwide platform for agricultural industries. We take 200 NMAIPs in China as objects. Through spatial analysis, the Herfindahl–Hirschman index, and the SBM-DEA model, we analyzed the development characteristics and regional differences of NMAIPs from the muti-level perspective of national planning, provincial coordination, and county implementation to propose policy recommendations aimed at sustainable and high-quality development. The results are as follows: (1) Regarding geospatial characteristics, NMAIPs are unevenly distributed, with a decreasing gradient from east to west. The direction is east (northward) to west (southward), consistent with the direction of the Hu line. The distribution density shows that the east is dense and the west is sparse. (2) For industrial concentration, the leading industries in NMAIPs tend to be homogenous. The HHI indicates that the homogenization of leading industries is widely represented in each province. The low oligopolistic areas are in the central and eastern regions of China, while the highly oligopolistic locations are in the western and northeastern provinces. (3) In inputs–outputs efficiency, the comprehensive technical efficiency is high but not optimal, while the distribution of values is high in the south and low in the north. Ten provinces are non-effective. According to inputs and outputs, the ineffective contribution of population of townships covered, occupied area and the capital from the collective economy are development barriers, and the high output value of NMAIPs cannot fully drive the employment and income of farmers. Further improvements are needed in terms of both pure technical efficiency and scale efficiency, and adjustments to scale operations should be in response to different returns to scale. Our research results provide policy recommendations for NMAIPs, including the establishment of a multi-level management mechanism, balancing regional development, diversifying and coordinating regional leading industries, and improving the efficiency of utilization factors. Full article

Genetic erosion of shea trees, which has been on-going at an alarming rate, has necessitated urgent conservation attentions. Owing to the vast geographical distribution of the species across Ghana, in situ germplasms conservation was established by tagging and monitoring selected trees annually. Technologies have been developed that enable shea germplasms to be grafted, allowing for the development of germplasm banks at the research station of the Cocoa Research Institute of Ghana, Bole. However, before these materials could be used in crop improvement programs, there is a need to evaluate them for key fruit traits relevant to the global shea business. This experiment was carried out to evaluate the tagged in situ shea trees for fruit and nut traits. Freshly harvested shea fruits were evaluated for their brix, pulp yield and kernel size properties to see if there was the needed diversity for crop improvement gains. Eight key traits were studied, with all showing significant difference, with high broad sense heritability and genetic advance for all the traits, indicating the potential for genetic gains in breeding programs. Qualitative analysis classified the fruits into five shapes, ellipsoid fruit shape was the most frequent observation (69.5%), while oblong was the least represented (1%). Fruit surface pubescence indicated that the surfaces without hairs (smooth) were slightly higher in number (52.6%) than the surface with hairs (rough), which were 47.4%. Pearson correlation studies showed a positive significant relationship between kernel weight and fruit weight (0.68), fruit length (0.48), fruit width (0.51), pulp weight (0.5) and shell weight (0.77). Key components responsible for total variations observed were decomposed from the first two principal components (PC), which cumulatively explained 78.4% of the total observed variation within the materials. PC1 alone contributed 46.4%, while PC2 contributed 32%. Fruit weight, fruit length, fruit width, pulp weight, nut weight, shell weight and kernel weight were contributing traits to variations observed in PC1, while brix and percent pulp contributed to the variations observed in PC2. Percent kernel to nut ratio contributed to the variations observed in PC3. Clustering of the germplasms showed no regular pattern based on location or any particular trait, indicating a high level of diversity at 58% of the Pearson dissimilarity index. Full article

Substitution of organic with inorganic fungicides (phosphites, Phi) does not change the efficiency of fertilizer nitrogen (N_f) in winter wheat. This hypothesis was tested in the 2016/2017 and 2017/2018 growing seasons. A two-factorial experiment with three phosphite variants (Cu–Phi, Mg–Phi, and Cu/Mg) and six plant protection methods (fungicides + Phi ⟶ reduced fungicide frequency + phosphite ⟶ phosphite). Grain yield decreased with increasing frequency of phosphites instead of fungicides. The decrease in yields was 3.6 t ha⁻¹ in the favorable 2016/2017 and 1.1 t ha⁻¹ in the dry 2017/2018. The primary reason for yield decrease in a given growing season was increased wheat infestation by pathogens. The direct cause was disturbances in the nitrogen status of wheat after flowering on treatments with a predominance of phosphites. The thousand grain weight (TGW) responded negatively to reduced fungicide application frequency. The critical stage in the assessment of pathogen pressure on wheat was the medium milk phase (BBCH 75). At this stage, indices of SPAD and leaf greenness together with indices of wheat infestation with pathogens allowed for a reliable prediction of both TGW and grain yield. It can be concluded that phosphites do not substitute organic fungicides in limiting pathogen pressure in winter wheat. Moreover, increased pressure of pathogens significantly reduces N_f productivity. Full article

Psyllaephagus sp. (Hymenoptera: Encyrtidae) is a newly recognized and important parasitoid of Cacopsylla chinensis (Yang and Li) (Hemiptera: Psyllidae), a pest of pear orchards. Its parasitism potential and rearing were studied in the laboratory. The studies showed that the most suitable hosts were fourth- and fifth-instar nymphs of pear psyllids (C. chinensis). The development duration of Psyllaephagus sp. females and males was 15.25 ± 0.37 and 13.57 ± 0.27 days when laying eggs in fourth-instar psyllid nymphs. The wasps did not survive longer than three days when they were fed only water, while they survived about an average of 20 days (23.20 ± 1.12 for females and 19.00 ± 0.80 for males) when fed 20% honey water. The provision of honey water could thus increase adult parasitoid longevity significantly. The lifetime fecundity of Psyllaephagus sp. females was 21.60 ± 0.88. Tests of parasitoid efficiency showed that the wasp’s functional response was Holling type II, with the number of hosts parasitized increasing with the host density to a maximum parasitism rate. The model suggested that a single Psyllaephagus sp. female could parasitize a maximum of 13.66 nymphs per day. The mutual interference of foraging Psyllaephagus sp. females occurred at high parasitoid densities. Psyllaephagus sp. has potential as a biocontrol agent for use against C. chinensis. Full article

Phytoliths are intra and extracellular siliceous deposits present in different plant tissues. Si uptake and transport are mediated by Lsi genes and its concentration is associated with forage quality. Our objective was to determine the phytolith content in seven Eragrostis curvula genotypes at the outbreak and re-growth stages to assess its relationship with the forage quality parameters and perform a genome-wide analysis to detect the presence/absence of Lsi genes. The mean values of the phytolith content of dry matter varied between 1.94–2.26% and 2.72–4.71% at the outbreak and re-growth stages, respectively, with highly significant differences among the genotypes and phenological stages. A highly negative correlation was observed in the phytolith content and in vitro dry matter digestibility and crude protein, revealing its importance as a selection parameter in breeding programs. A positive correlation was obtained between the phytolith content and lignin, neutral detergent fiber, and acid detergent fiber. The main morphotypes of the phytoliths included saddle-shaped, bulliform, and acicular cells. Genes Lsi1, Lsi2, Lsi3, and Lsi6, previously reported in silica uptake, were identified and compared with related species, being the gene sequences highly conserved, meaning that its accumulation is probably due to differences in the gene expression or different allelic variants among cultivars. Full article