Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat
Abstract
:1. Introduction
2. Results
2.1. Sequence Analysis
2.2. Creation of ΔFgCWM1 and C-FgCWM1
2.3. Effect of FgCWM1 on Mycelial Growth
2.4. Effect of FgCWM1 on Fungal Cell Wall Development
2.5. FgCWM1 Encodes a Cell Wall Mannoprotein
2.6. Effect of FgCWM1 on Pathogenicity
3. Discussion
4. Materials and Methods
4.1. Materials and Growth Conditions
4.2. Sequence Analysis
4.3. Construction of Deletion and Complementation Mutants
4.4. FgCWM1 Expression in S. cerevisiae
4.5. Determination of Mannose and Protein Contents in Fungal Cell Wall
4.6. Microscopic Assay
4.7. Virulence Assay and DON Measurement
4.8. Gene Expression Analysis
4.9. Quantification of SA in Wheat Spikes
4.10. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Primer | Sequence (5′– 3′) | Reference |
---|---|---|
FgCWM1-Up-F | GCGGGCCCTACTCAGGGTAACGGAAAGG | This study |
FgCWM1-Up-R | GCGAGCTCACAATGCAGCTCAATGTCG | |
FgCWM1-Down-F | GGAAGCTTCAACCCAGACCTACCCA | This study |
FgCWM1-Down-R | GGACTAGTGAAGAGCAGCGAACCAG | |
P5 | TGATAATAATGTCCTCGTTCC | This study |
P6 | TGACGAACTGTAAGTCGGATA | |
P7 | ACCGAACTTCAAGACACCA | This study |
P8 | CAACGGCCTCAACCTACT | |
R-FgCWM1-F | AACCATGGATGAAGTTCTCCGCTGC | This study |
R-FgCWM1-R | GGCCATGGAAGCATCCTTCAGAAGAGGT | |
SS-FgCWM1-F | GCGAGCTCGCATGAAGTTCTCCGCTGC | This study |
SS-FgCWM1-R | GGGGATCCAAGCATCCTTCAGAAGAGGT | |
Fg-GAPDH-F | TGACTTGACTGTTCGCCTCGAGAA | [22] |
Fg-GAPDH-R | ATGGAGGAGTTGGTGTTGCCGTTA | |
Fg-β-tubulin-F | GTTGATCTCCAAGATCCGTG | [22] |
Fg-β-tubulin-R | CATGCAAATGTCGTAGAGGG | |
Fg-Factor1-F | CCTCCAGGATGTCTACAAGA | [22] |
Fg-Factor1-R | CTCAACGGACTTGACTTCAG | |
RJ-FgCWM1-F | GCTGGTGCCGAGGCTGTT | This study |
RJ-FgCWM1-R | GGTCATCGCAGGTGTTTCCA | |
Aox-F | GACTTGTCATGGTAGATGCCTG | [22] |
Aox-R | CAGGACGAGCATAACCATTCTC | |
w-GAPDH-F | AACTGTTCATGCCATCACTGCCAC | [22] |
w-GAPDH-R | AGGACATACCAGTGAGCTTGCCAT | |
hn-RNP-Q-F | TCACCTTCGCCAAGCTCAGAACTA | [22] |
hn-RNP-Q-R | AGTTGAACTTGCCCGAAACATGCC |
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Zhang, Y.-Z.; Chen, Q.; Liu, C.-H.; Lei, L.; Li, Y.; Zhao, K.; Wei, M.-Q.; Guo, Z.-R.; Wang, Y.; Xu, B.-J.; et al. Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat. Toxins 2019, 11, 628. https://doi.org/10.3390/toxins11110628
Zhang Y-Z, Chen Q, Liu C-H, Lei L, Li Y, Zhao K, Wei M-Q, Guo Z-R, Wang Y, Xu B-J, et al. Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat. Toxins. 2019; 11(11):628. https://doi.org/10.3390/toxins11110628
Chicago/Turabian StyleZhang, Ya-Zhou, Qing Chen, Cai-Hong Liu, Lu Lei, Yang Li, Kan Zhao, Mei-Qiao Wei, Zhen-Ru Guo, Yan Wang, Bin-Jie Xu, and et al. 2019. "Fusarium graminearum FgCWM1 Encodes a Cell Wall Mannoprotein Conferring Sensitivity to Salicylic Acid and Virulence to Wheat" Toxins 11, no. 11: 628. https://doi.org/10.3390/toxins11110628