Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity
Abstract
:1. Introduction
2. Results
2.1. Interaction between FLs and BSA
2.2. Interaction between BSA and (−)-Epicatechin and Procyanidins
2.3. Docking Studies
2.4. Influence of BSA on Cytotoxicity of FLs
2.5. Comparison of Cytotoxicity of Procyanidins
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Analysis of Fluorescence Quenching of BSA
4.3. Molecular Docking Experiment
4.4. Cytotoxicity Test
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kq (×10−3, L·mol−1·s−1) | Coefficient of Determination (R2) | |
---|---|---|
(−)-epicatechin (EC) | 7.44 ± 2.13 1 | 0.0157 |
procyanidin B2 (B2) | 16.35 ± 4.37 | 0.9201 |
procyandin C1 (C1) | 14.33 ± 4.98 | 0.8957 |
cinnamtannin A2 (A2) | 29.28 ± 7.47 | 0.9987 |
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Fujii, Y.; Suhara, Y.; Sukikara, Y.; Teshima, T.; Hirota, Y.; Yoshimura, K.; Osakabe, N. Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity. Molecules 2019, 24, 3667. https://doi.org/10.3390/molecules24203667
Fujii Y, Suhara Y, Sukikara Y, Teshima T, Hirota Y, Yoshimura K, Osakabe N. Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity. Molecules. 2019; 24(20):3667. https://doi.org/10.3390/molecules24203667
Chicago/Turabian StyleFujii, Yasuyuki, Yoshitomo Suhara, Yusuke Sukikara, Tomohiro Teshima, Yoshihisa Hirota, Kenjiro Yoshimura, and Naomi Osakabe. 2019. "Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity" Molecules 24, no. 20: 3667. https://doi.org/10.3390/molecules24203667