Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemical Identification of the Isolated Compounds from C. walteri
2.2. Regulatory Effects of the Compounds on Mesenchymal Stem Cell Differentiation into Adipocytes and Osteoblasts
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Plant Material
3.3. Extraction and Isolation
3.3.1. Cornusalterin N (1)
3.3.2. Cornusalterin O (2)
3.3.3. Cornusalterin P (3)
3.4. Computational Analysis
3.5. Cell Culture and Differentiation
3.6. Alkaline Phosphatase (ALP) Staining
3.7. Quantification of Alkaline Phosphatase (ALP) Activity
3.8. Oil Red O (ORO) Staining
3.9. Cell Viability
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Position | 1 | 2 | 3 | |||
---|---|---|---|---|---|---|
δH | δC | δH | δC | δH | δC | |
1 | 1.64 m 1.15 m | 39.2 t | 1.76 m 1.28 m | 40.1 t | 1.69 m 1.07 m | 39.3 t |
2 | 1.75 m 1.65 m | 27.5 t | 2.51 m, 2.43 m | 34.3 t | 1.82 m, 1.64 m | 27.6 t |
3 | 3.22 dd (11.5 5.0) | 78.9 d | 218.5 s | 3.20 dd (11.5, 5.0) | 79.1 d | |
4 | 38.9 s | 47.6 s | 39.2 s | |||
5 | 0.75 m | 55.8 d | 1.36 m | 55.6 d | 0.73 m | 56.1 d |
6 | 1.72 m 1.50 m | 18.3 t | 1.75 m, 1.53 m | 22.2 t | 1.70 m, 1.48 m | 18.5 t |
7 | 1.52 m, 1.28 m | 24.8 t | 1.52 m, 1.30 m | 25.0 t | 1.51 m, 1.28 m | 25.0 t |
8 | 1.43 m | 42.4 d | 1.41 m | 42.7 d | 1.40 m | 42.6 d |
9 | 1.34 m | 50.6 d | 1.35 m | 50.2 d | 1.32 m | 50.9 d |
10 | 37.1 s | 37.0 s | 37.4 s | |||
11 | 1.53 m, 1.06 m | 21.5 t | 1.46 m, 1.09 m | 19.9 t | 1.45 m, 1.07 m | 21.7 t |
12 | 1.74 m, 1.22 m | 25.4 t | 1.71 m, 1.23 m | 25.1 t | 1.73 m, 1.21 m | 25.1 t |
13 | 40.3 s | 40.5 s | 40.6 s | |||
14 | 50.3 s | 50.4 s | 50.5 s | |||
15 | 1.52 m, 1.29 m | 31.2 t | 1.56 m, 1.30 m | 31.3 t | 1.49 m, 1.27 m | 31.3 t |
16 | 1.82 m, 1.21 m | 27.4 t | 1.86 m, 1.23 m | 27.7 t | 1.84 m, 1.25 m | 27.5 t |
17 | 1.49 m | 50.1 d | 1.74, m | 50.1 d | 1.72 m | 50.1 d |
18 | 0.97 s | 15.5 q | 1.00 s | 15.4 q | 0.96 s | 15.7 q |
19 | 0.86 s | 16.2 q | 0.95 s | 16.2 q | 0.85 s | 16.4 q |
20 | 75.4 s | 75.2 s | 75.3 s | |||
21 | 1.16 s | 25.3 q | 1.14 s | 26.1 q | 1.13 s | 26.0 q |
22 | 1.42 m | 36.3 t | 2.21 m | 43.6 t | 2.20 m | 43.6 t |
23 | 1.83 m, 1.61 m | 29.3 t | 5.70 m | 122.5 d | 5.70 m | 122.6 d |
24 | 4.06 t (6.0) | 76.2 d | 5.71 m | 142.3 d | 5.71 m | 142.2 d |
25 | 147.6 s | 71.0 s | 71.0 s | |||
26 | 4.96 br s, 4.86 br s | 110.7 t | 1.33 s | 30.2 q | 1.33 s | 30.2 q |
27 | 1.75 s | 18.0 q | 1.33 s | 30.1 q | 1.33 s | 30.1 q |
28 | 0.99 s | 28.0 q | 1.08 s | 27.0 q | 0.97 s | 28.2 q |
29 | 0.79 s | 15.3 q | 1.04 s | 21.2 q | 0.77 s | 15.6 q |
30 | 0.89 s | 16.4 q | 0.88 s | 16.5 q | 0.87 s | 16.6 q |
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Lee, S.R.; Choi, E.; Jeon, S.H.; Zhi, X.Y.; Yu, J.S.; Kim, S.-H.; Lee, J.; Park, K.-M.; Kim, K.H. Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations. Molecules 2018, 23, 2732. https://doi.org/10.3390/molecules23112732
Lee SR, Choi E, Jeon SH, Zhi XY, Yu JS, Kim S-H, Lee J, Park K-M, Kim KH. Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations. Molecules. 2018; 23(11):2732. https://doi.org/10.3390/molecules23112732
Chicago/Turabian StyleLee, Seoung Rak, Eunyong Choi, Se Hun Jeon, Xue Yan Zhi, Jae Sik Yu, Seon-Hee Kim, Jeongmi Lee, Ki-Moon Park, and Ki Hyun Kim. 2018. "Tirucallane Triterpenoids from the Stems and Stem Bark of Cornus walteri that Control Adipocyte and Osteoblast Differentiations" Molecules 23, no. 11: 2732. https://doi.org/10.3390/molecules23112732