A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae
Abstract
:1. Introduction
2. Results
2.1. Establishment of BSH Database in Lactobacillaceae
2.2. Phylogenetic Analysis
2.3. Sequence Analysis of BSH
2.4. Genome Survey of bsh Genes in Lactobacillaceae Species
3. Discussion
4. Materials and Methods
4.1. Establishment of BSH–SDL
4.2. Identification of BSH in Lactobacillaceae
4.3. Genomic Location of bsh
4.4. Sequence Alignment and Phylogenetic Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CVD | cardiovascular disease |
TC | total cholesterol |
LDL-C | low-density lipoprotein cholesterol |
BAs | bile acids |
CA | cholic acid |
CDCA | chenodeoxycholic acid |
TCA | taurocholic acid |
TCDCA | taurochenodeoxycholic acid |
GCA | glycocholic acid |
GCDCA | glycochenodeoxycholic acid |
BSH | bile salt hydrolase |
BSH–SDL | bile salt hydrolase seed database of Lactobacillaceae |
FXR | farnesoid X receptor |
GPBAR1/TGR5 | G-protein-coupled bile acid receptor 1 |
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Sample Availability: Samples of the compounds are not available. |
Species | BSH Subtypes | Tolerable Conditions | Substrate Specificities | Strains | |||||
---|---|---|---|---|---|---|---|---|---|
A | B | C | D | E | Bile Salt (%) | pH | |||
Lactobacillus plantarum | 1 | 2 | 1 | 1.0 | 2.0 | GCA | ST-III [20] | ||
1 | 2 | 1 | - | - | GCA | WCFS1 [19] | |||
1 | 2 | 1 | 0.3 | 2.0 | - | LZ95 [26] | |||
1 | 1 | 1 | 1.0 | 2.0 | - | NCU116 [27] | |||
Lactobacillus acidophilus | 2 | 0.2 | - | TCA, GCA, TCDCA | NCFM [22] | ||||
2 | 0.3 | 2.0 | - | DSM 20079 [28] | |||||
Lactobacillus johnsonii | 2 | 0.5 | - | - | PF01 [29] | ||||
2 | 0.5 | 2.0 | GCDCA, TCDCA | La1 (also known as NCC533) [30] | |||||
Lactobacillus rhamnosus | 1 | 2.0 | 2.5 | - | GG (ATCC 53103) [31] |
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Liang, L.; Yi, Y.; Lv, Y.; Qian, J.; Lei, X.; Zhang, G. A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae. Molecules 2018, 23, 1157. https://doi.org/10.3390/molecules23051157
Liang L, Yi Y, Lv Y, Qian J, Lei X, Zhang G. A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae. Molecules. 2018; 23(5):1157. https://doi.org/10.3390/molecules23051157
Chicago/Turabian StyleLiang, Lifeng, Yunhai Yi, Yunyun Lv, Junwei Qian, Xuejing Lei, and Gengyun Zhang. 2018. "A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae" Molecules 23, no. 5: 1157. https://doi.org/10.3390/molecules23051157