Transcriptome Profile in Unilateral Adrenalectomy-Induced Compensatory Adrenal Growth in the Rat
Abstract
:1. Introduction
2. Results
2.1. Unilateral Adrenalectomy-Induced Compensatory Adrenal Growth Increases the Weight of the Remaining Gland 24 and 72 h after Hemiadrenalectomy
2.2. Unilateral Adrenalectomy Exerts a Modest Influence on the Entire Adrenal Gland Gene Expression Profile
2.3. Unilateral Adrenalectomy-Induced Compensatory Growth Affects the Expression of Genes Involved in Lipid and Steroid, Circadian Rhythm, Stress, and Cell Cycle Regulations
3. Discussion
4. Materials and Methods
4.1. Animals and Experiments
4.2. Reagents
4.3. Unilateral Adrenalectomy
4.4. RNA Extraction
4.5. Microarray Expression Study
4.6. Microarray Data Analysis
4.7. Assignment of Differentially Expressed Genes to Relevant Gene Ontology (GO) Terms
4.8. Statistics
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
ACTH | adrenocorticotropic hormone |
DEG | differentially expressed genes |
FDR | false discovery rate |
GO | gene ontology |
DAVID | database for annotation, visualization and integrated discovery |
BP | biological process |
Cyp51 | cytochrome P450, family 51, subfamily A, polypeptide 1 |
Slfn13 | Schlafen family member 13 |
Aox1 | aldehyde oxidase 1 |
Arntl | aryl hydrocarbon receptor nuclear translocator-like protein 1 |
Nr1d1 | nuclear receptor subfamily 1 group D member 1 |
Nr1d2 | nuclear receptor subfamily 1 group D member 2 |
Ki-67 | marker of proliferation Ki-67 |
Ccna2 | cyclin A2 |
Cdkn1c | cyclin-dependent kinase inhibitor 1C |
StAR | steroidogenic acute regulatory protein |
Cyp11a1 | cytochrome P450 family 11 subfamily A member 1 (cholesterol 20–22 desmolase) |
Cyp11b1 | cytochrome P450 family 11 subfamily B member 1 |
Cyp11b2 | cytochrome P450 family 11 subfamily B member 2 (aldosterone synthase) |
Lipe | hormone-sensitive lipase |
Uts2 | urotensin 2 |
Y1 | mouse adrenocortical cell line |
Clock | Clock circadian regulator |
Bmal1 | brain and muscle ARNT-like 1 |
Cry1 | cryptochrome circadian clock 1 |
Cry2 | cryptochrome circadian clock 2 |
Per1 | period circadian clock 1 |
Per2 | period circadian clock 2 |
Per3 | period circadian clock 3 |
C57BL/6J | inbred strain of laboratory mouse |
POMC | pro-opiomelanocortin |
MSH | melanocyte-stimulating hormone (melanotropin) |
CEL | Affymetrix data file format |
RMA | robust multi-array average |
FC | fold change |
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24 h/c | 72 h/c | ||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Fold Change | Adj. p Value | Gene Symbol | Gene Name | Fold Change | Adj. p Value |
Uts2 | urotensin 2 | 3.52 | 0.00147 | Terc | telomerase RNA component | 5.31 | 0.00134 |
Per2 | period circadian clock 2 | 2.61 | 0.01402 | Spetex-2H | Spetex-2H protein | 3.92 | 0.00154 |
Qrfpr | pyroglutamylated RFamide peptide receptor | 2.35 | 0.03213 | Olr522 | olfactory receptor 522 | 3.79 | 0.09894 |
Ptprn | protein tyrosine phosphatase, receptor type, N | 2.20 | 0.01550 | Spetex-2D | Spetex-2D protein | 3.76 | 0.00134 |
Slfn13 | schlafen family member 13 | 2.14 | 0.09564 | Spetex-2F | Spetex-2F protein | 3.71 | 0.00402 |
Hsd17b7 | hydroxysteroid (17-beta) dehydrogenase 7 | 2.13 | 0.06722 | Spetex-2G | Spetex-2G protein | 2.98 | 0.01111 |
Per3 | period circadian clock 3 | 1.98 | 0.00954 | Slfn13 | schlafen family member 13 | 2.64 | 0.00333 |
Cyp51 | cytochrome P450, family 51 | 1.93 | 0.03407 | Igha | immunoglobulin heavy chain, alpha | 2.11 | 0.01254 |
Ezh2 | enhancer of zeste 2 polycomb repressive complex 2 subunit | 1.85 | 0.03407 | Vom2r73 | vomeronasal 2 receptor, 73 | 2.10 | 0.02229 |
Giotl | gonadotropin inducible ovarian transcription factor 1 | 1.83 | 0.06722 | Vom2r72 | vomeronasal 2 receptor, 72 | 1.93 | 0.04645 |
Cyp4f4 | cytochrome P450, family 4, subfamily f, polypeptide 4 | −1.51 | 0.31696 | Hhex | hematopoietically expressed homeobox | −2.07 | 0.00573 |
Adamts2 | ADAM metallopeptidase with thrombospondin type 1 motif, 2 | −1.51 | 0.03504 | Pletl | placenta expressed transcript 1 | −2.20 | 0.09155 |
Cox8b | cytochrome c oxidase, subunit Vlllb | −1.52 | 0.23584 | Ass1 | argininosuccinate synthase 1 | −2.21 | 0.07197 |
MiR3120 | microRNA miR-3120 | −1.54 | 0.14294 | Osbpl6 | oxysterol binding protein-like 6 | −2.22 | 0.08097 |
Ass1 | argininosuccinate synthase 1 | −1.58 | 0.64063 | Perl | period circadian clock 1 | −2.23 | 0.00748 |
Aox1 | aldehyde oxidase 1 | −1.62 | 0.06324 | Cyp4f4 | cytochrome P450, family 4, subfamily f, polypeptide 4 | −2.32 | 0.00161 |
Sult1a1 | sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1 | −1.84 | 0.01553 | Gadd45g | growth arrest and DNA-damage-inducible, gamma | −2.53 | 0.02125 |
H23r | interleukin 23 receptor | −2.04 | 0.01550 | Nr4a1 | nuclear receptor subfamily 4, group A, member 1 | −2.74 | 0.01979 |
Arntl | aryl hydrocarbon receptor nuclear translocator-like | −2.16 | 0.00954 | Slc9a3 | solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 | −2.88 | 0.00048 |
Npas2 | neuronal PAS domain protein 2 | −2.18 | 0.00147 | Ddit4 | DNA-damage-inducible transcript 4 | −4.04 | 0.00344 |
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Jopek, K.; Tyczewska, M.; Celichowski, P.; Malendowicz, L.K.; Rucinski, M. Transcriptome Profile in Unilateral Adrenalectomy-Induced Compensatory Adrenal Growth in the Rat. Int. J. Mol. Sci. 2018, 19, 1111. https://doi.org/10.3390/ijms19041111
Jopek K, Tyczewska M, Celichowski P, Malendowicz LK, Rucinski M. Transcriptome Profile in Unilateral Adrenalectomy-Induced Compensatory Adrenal Growth in the Rat. International Journal of Molecular Sciences. 2018; 19(4):1111. https://doi.org/10.3390/ijms19041111
Chicago/Turabian StyleJopek, Karol, Marianna Tyczewska, Piotr Celichowski, Ludwik K. Malendowicz, and Marcin Rucinski. 2018. "Transcriptome Profile in Unilateral Adrenalectomy-Induced Compensatory Adrenal Growth in the Rat" International Journal of Molecular Sciences 19, no. 4: 1111. https://doi.org/10.3390/ijms19041111