Antinociceptive Effect of Intrathecal Microencapsulated Human Pheochromocytoma Cell in a Rat Model of Bone Cancer Pain
Abstract
:1. Introduction
2. Results and Discussion
2.1. Bone Destruction Evaluation by Radiograph
2.2. Time-Course of Mechanical Hyperalgesia Induced by Walker 256 Inoculation
2.3. Secretion of Micro-HPC (Microencapsulated Human Pheochromocytoma Cell) in Vivo and in Vitro
Time (Pre- and Post-Microencapsulation of HPC) | Met-Enkephalin (pg/µL) | Norepinephrine (ng/µL) |
---|---|---|
Pre-microencapsulation | 3.14 ± 1.05 | 3.62 ± 0.61 |
Day2 (post-microencapsulation) | 3.23 ± 0.79 | 3.59 ± 0.43 |
Day4 (post-microencapsulation) | 3.09 ± 0.63 | 3.65 ± 0.95 |
Day6 (post-microencapsulation) | 3.19 ± 0.48 | 3.54 ± 0.37 |
Group | Met-Enkephalin (pg/µL) | Norepinephrine (ng/µL) |
---|---|---|
CIP + empty capsule | 6.50 + 0.21 | 2.85 + 0.15 |
CIP + micro-HPC | 8.93 + 0.65 * | 5.54 + 0.73 * |
2.4. Intrathecal Injection of Micro-HPC Attenuated Mechanical Allodynia Induced by Walker 256 Inoculation
2.5. Antagonizing Opioid Signaling Reversed the Analgesic Effect of Micro-HPC on Mechanical Hyperalgesia
2.6. Discussion
3. Experimental Section
3.1. Animals
3.2. Cells
3.3. Microencapsulation
3.4. Bone Cancer Model and Radiology
3.5. Mechanical Hyperalgesia Test
3.6. Thermal Hyperalgesia
3.7. Secretion Study of Cells in Vitro and in Vivo
3.8. Antagonist Study
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Li, X.; Li, G.; Wu, S.; Zhang, B.; Wan, Q.; Yu, D.; Zhou, R.; Ma, C. Antinociceptive Effect of Intrathecal Microencapsulated Human Pheochromocytoma Cell in a Rat Model of Bone Cancer Pain. Int. J. Mol. Sci. 2014, 15, 12135-12148. https://doi.org/10.3390/ijms150712135
Li X, Li G, Wu S, Zhang B, Wan Q, Yu D, Zhou R, Ma C. Antinociceptive Effect of Intrathecal Microencapsulated Human Pheochromocytoma Cell in a Rat Model of Bone Cancer Pain. International Journal of Molecular Sciences. 2014; 15(7):12135-12148. https://doi.org/10.3390/ijms150712135
Chicago/Turabian StyleLi, Xiao, Guoqi Li, Shaoling Wu, Baiyu Zhang, Qing Wan, Ding Yu, Ruijun Zhou, and Chao Ma. 2014. "Antinociceptive Effect of Intrathecal Microencapsulated Human Pheochromocytoma Cell in a Rat Model of Bone Cancer Pain" International Journal of Molecular Sciences 15, no. 7: 12135-12148. https://doi.org/10.3390/ijms150712135