Monocarboxylate Transporter 4 Regulates Glioblastoma Motility and Monocyte Binding Ability
Abstract
:1. Introduction
2. Results
2.1. MCT4 Expression Correlates with Pathological Grades of Human Glioma
2.2. MCT4 Is Involved in the Hypoxia-Enhanced GBM Migration and Monocyte Adhesion
2.3. HIF-1α Is Involved in the Hypoxia-Induced MCT4 Expression in GBM
2.4. Acetylated STAT3 Is Involved in Hypoxia-Induced HIF-1α and MCT4 Expression in GBM
2.5. Activation of FAK and AKT is Involved in Hypoxia-Induced HIF-1α and MCT4 Expression in GBM
2.6. Activation of EGFR Is Involved in Hypoxia-Induced HIF-1α and MCT4 Expression in GBM
2.7. Expression of MCT4 Is Elevated in the GBM Mouse Model
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Animals
4.3. Cell Culture
4.4. Cytosolic and Nuclear Extracts
4.5. Monocyte-Binding Assay
4.6. Western Blotting
4.7. Reverse Transcription and Real-Time PCR
4.8. Luciferase Reporter Assay
4.9. Cell Transfection
4.10. Cell Migration Assay
4.11. Chromatin Immunoprecipitation (ChIP) Assay
4.12. GEO Gene Expression Database
4.13. Intracranial Tumor Implantation
4.14. Immunohistochemistry
4.15. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lai, S.-W.; Lin, H.-J.; Liu, Y.-S.; Yang, L.-Y.; Lu, D.-Y. Monocarboxylate Transporter 4 Regulates Glioblastoma Motility and Monocyte Binding Ability. Cancers 2020, 12, 380. https://doi.org/10.3390/cancers12020380
Lai S-W, Lin H-J, Liu Y-S, Yang L-Y, Lu D-Y. Monocarboxylate Transporter 4 Regulates Glioblastoma Motility and Monocyte Binding Ability. Cancers. 2020; 12(2):380. https://doi.org/10.3390/cancers12020380
Chicago/Turabian StyleLai, Sheng-Wei, Hui-Jung Lin, Yu-Shu Liu, Liang-Yo Yang, and Dah-Yuu Lu. 2020. "Monocarboxylate Transporter 4 Regulates Glioblastoma Motility and Monocyte Binding Ability" Cancers 12, no. 2: 380. https://doi.org/10.3390/cancers12020380