Imaging Collagen Alterations in STICs and High Grade Ovarian Cancers in the Fallopian Tubes by Second Harmonic Generation Microscopy
Abstract
:1. Introduction
2. Results
2.1. SHG Sampling
2.2. Gray Level Co-Occurrence Matrix Analysis
- (i)
- Angular Second Moment (ASM) and Energy measures the number of repeated pairs. The energy (square root of ASM) will be high if the occurrence of repeated pixel pairs is high.
- (ii)
- Inverse Difference Moment (IDM) is related to the smoothness or homogeneity across the image and will be high if the gray levels of the pixel pairs are similar.
- (iii)
- Contrast is a measure of the local contrast of an image and will be low if the gray levels of each pixel pair are similar.
- (iv)
- Entropy measures the randomness of a gray level distribution and will be high if the gray levels are distributed randomly throughout the image.
- (v)
- Correlation measures the linear dependency of gray levels on those of neighboring pixels.
2.3. Two-Dimensional Fast Fourier Transform Analysis
2.4. CT-FIRE Analysis
2.5. Classification by Linear Discriminate Analysis
3. Discussion
4. Methods
4.1. Fallopian Tube Tissues
4.2. SHG Microscopy
4.3. Image Analysis
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Rentchler, E.C.; Gant, K.L.; Drapkin, R.; Patankar, M.; J. Campagnola, P. Imaging Collagen Alterations in STICs and High Grade Ovarian Cancers in the Fallopian Tubes by Second Harmonic Generation Microscopy. Cancers 2019, 11, 1805. https://doi.org/10.3390/cancers11111805
Rentchler EC, Gant KL, Drapkin R, Patankar M, J. Campagnola P. Imaging Collagen Alterations in STICs and High Grade Ovarian Cancers in the Fallopian Tubes by Second Harmonic Generation Microscopy. Cancers. 2019; 11(11):1805. https://doi.org/10.3390/cancers11111805
Chicago/Turabian StyleRentchler, Eric C., Kristal L. Gant, Ronny Drapkin, Manish Patankar, and Paul J. Campagnola. 2019. "Imaging Collagen Alterations in STICs and High Grade Ovarian Cancers in the Fallopian Tubes by Second Harmonic Generation Microscopy" Cancers 11, no. 11: 1805. https://doi.org/10.3390/cancers11111805