Sustainable Synthesis of FITC Chitosan-Capped Gold Nanoparticles for Biomedical Applications
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of Gold Nanoparticles by Chitosan Using a Microwave Oven
2.2. Functionalization of AuNPs@CS by FITC
2.3. Characterization of AuNPs@CS-FITC
2.4. Cell Culture
2.5. Metal Content Measurement by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES)
2.6. MTT Assay
2.7. Confocal Microscopy Analysis and Fluorescence Quantification
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Size (nm) in Water | ζ-Potential (mV ±) in Water | |
AuNPs@CS | 15 ± 2 | +32 ± 2 |
Size (nm) in DMEM | ζ-Potential (mV ±) in DMEM | |
AuNPs@CS | 23 ± 4 | −30 ± 3 |
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De Matteis, V.; Rizzello, L.; Cascione, M.; Pellegrino, P.; Singh, J.; Manno, D.; Rinaldi, R. Sustainable Synthesis of FITC Chitosan-Capped Gold Nanoparticles for Biomedical Applications. Clean Technol. 2022, 4, 942-953. https://doi.org/10.3390/cleantechnol4040058
De Matteis V, Rizzello L, Cascione M, Pellegrino P, Singh J, Manno D, Rinaldi R. Sustainable Synthesis of FITC Chitosan-Capped Gold Nanoparticles for Biomedical Applications. Clean Technologies. 2022; 4(4):942-953. https://doi.org/10.3390/cleantechnol4040058
Chicago/Turabian StyleDe Matteis, Valeria, Loris Rizzello, Mariafrancesca Cascione, Paolo Pellegrino, Jagpreet Singh, Daniela Manno, and Rosaria Rinaldi. 2022. "Sustainable Synthesis of FITC Chitosan-Capped Gold Nanoparticles for Biomedical Applications" Clean Technologies 4, no. 4: 942-953. https://doi.org/10.3390/cleantechnol4040058