Inflammatory Cytokines Facilitate the Sensitivity of P2X7 Receptors Toward Extracellular ATP at Neural Progenitor Cells of the Rodent Hippocampal Subgranular Zone
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Hippocampal Brain Slices and the Corresponding Organotypic Slice Cultures
2.2. Whole-Cell Patch-Clamp Recordings
2.3. Drug Application Protocols
2.4. Multiple Immunofluorescence Labeling Under Confocal Microscopic Observation
2.5. Materials
2.6. Statistics
3. Results
3.1. Sensitivity Increase of P2X7Rs at SGZ NPCs in Organotypic Hippocampal Slices Caused by Pre-Incubation with Lipopolysaccharide and Cytokines
3.2. Sensitivity Increase of P2X7Rs at SGZ NPCs in Organotypic Hippocampal Slices Caused Pre-Incubation with Growth Factors, but not by Pre-Incubation with Reactive Oxygen Species or Metabolic Inhibitors
3.3. P2X7R Immunoreactivity in Organotypic Hippocampal Slice Cultures
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Liu, J.; Khan, M.T.; Tang, Y.; Franke, H.; Illes, P. Inflammatory Cytokines Facilitate the Sensitivity of P2X7 Receptors Toward Extracellular ATP at Neural Progenitor Cells of the Rodent Hippocampal Subgranular Zone. Neuroglia 2018, 1, 258-270. https://doi.org/10.3390/neuroglia1010017
Liu J, Khan MT, Tang Y, Franke H, Illes P. Inflammatory Cytokines Facilitate the Sensitivity of P2X7 Receptors Toward Extracellular ATP at Neural Progenitor Cells of the Rodent Hippocampal Subgranular Zone. Neuroglia. 2018; 1(1):258-270. https://doi.org/10.3390/neuroglia1010017
Chicago/Turabian StyleLiu, Juan, Muhammad Tahir Khan, Yong Tang, Heike Franke, and Peter Illes. 2018. "Inflammatory Cytokines Facilitate the Sensitivity of P2X7 Receptors Toward Extracellular ATP at Neural Progenitor Cells of the Rodent Hippocampal Subgranular Zone" Neuroglia 1, no. 1: 258-270. https://doi.org/10.3390/neuroglia1010017