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plasma 2018, 1(1), 2; doi:10.3390/plasma1010002

Endothelialization of Polyethylene Terephthalate Treated in SO2 Plasma Determined by the Degree of Material Cytotoxicity

Department of surface engineering and optoelectronics, Jozef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia
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Received: 18 October 2017 / Revised: 4 December 2017 / Accepted: 8 December 2017 / Published: 9 December 2017
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Abstract

Improving the biocompatibility of polyethylene terephthalate (PET) vascular grafts is an important task for avoiding thrombus formation. Therefore, SO2 plasma at various treatment periods were used to modify PET surface properties by forming sulfate functional groups. These groups were shown to act antithrombogenically, ensuring good hemocompatibility of the materials, although the biocompatibility of such materials still remains a mystery. For this reason, the adhesion and viability of HUVEC cells on SO2 plasma-modified PET surfaces were studied, and the possible toxicity of the tested material was determined using two different assays, MTT (metabolic activity assay) and SRB (in-vitro toxicology assay). Changes in chemical composition, morphology and wettability were determined as well. Improved endothelialization was observed for all plasma-treated samples, with the most optimal being the sample treated for 80 s, which can be explained by it having the best combination of surface functionalization, roughness and morphology. Furthermore, toxicity was observed to some extent on the sample treated for 160 s, indicating the lowest cell density among the plasma-treated samples. X-ray photoelectron spectroscopy showed increased oxygen and sulfur content on the surface, which was independent on treatment time. Surface roughness of the plasma-treated samples increased, reaching its maximum after 80 s of treatment, and decreased thereafter. View Full-Text
Keywords: polymer; polyethylene terephthalate (PET); SO2 plasma treatment; surface modification; X-ray photoelectron spectroscopy (XPS); atomic force microscopy (AFM); human umbilical vein endothelial cell (HUVEC) proliferation; MTT and SRB assay; cytotoxicity polymer; polyethylene terephthalate (PET); SO2 plasma treatment; surface modification; X-ray photoelectron spectroscopy (XPS); atomic force microscopy (AFM); human umbilical vein endothelial cell (HUVEC) proliferation; MTT and SRB assay; cytotoxicity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Vesel, A.; Recek, N.; Motaln, H.; Mozetic, M. Endothelialization of Polyethylene Terephthalate Treated in SO2 Plasma Determined by the Degree of Material Cytotoxicity. plasma 2018, 1, 2.

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