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Proceedings 2017, 1(4), 515; doi:10.3390/proceedings1040515

Real-Time Monitoring of Stem Cells by Diamond-Based Impedance Sensors

1
Institute of Physic, Czech Academy of Sciences v.v.i., Cukrovarnická 10, 162 00 Prague 6, Czech Republic
2
Faculty of Electrical Engineering, Czech Technical University in Prague, Technická 2, 166 27 Prague, Czech Republic
3
Institute of Physiology, Czech Academy of Sciences v.v.i., Videnska 1083, 14220 Prague 4, Czech Republic
4
Faculty of Biomedical Engineering, Czech Technical University in Prague, nám. Sítná 3105, 272 01 Kladno, Czech Republic
5
Faculty of Civil Engineering, Czech Technical University in Prague, 166 29 Prague, Czech Republic
Presented at the Eurosensors 2017 Conference, Paris, France, 3–6 September 2017.
*
Author to whom correspondence should be addressed.
Published: 23 August 2017
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Abstract

Cell-based impedance spectroscopy is a promising label-free method for electrical monitoring of cell activity. Here we present a diamond-based impedance sensor with built-in gold interdigitated electrodes (IDEs) as a promising platform for simultaneous electrical and optical monitoring of adipose-derived stem cells (ASC). The impedance spectra were collected in a wide frequency range (from 100 Hz to 50 kHz) for 27 h of cultivation in chambers designed for static cultivation. Absolute impedance spectra were analyzed in terms of measurement frequencies and cell properties monitored by high-resolution digital camera.
Keywords: diamond film; biosensor; stem cells; impedance spectroscopy diamond film; biosensor; stem cells; impedance spectroscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Procházka, V.; Matějka, R.; Ižák, T.; Szabó, O.; Štěpanovská, J.; Bačáková, L.; Kromka, A. Real-Time Monitoring of Stem Cells by Diamond-Based Impedance Sensors. Proceedings 2017, 1, 515.

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